Xenobiotic transcription factors CncC and maf regulate expression of CYP321A16 and CYP332A1 that mediate chlorpyrifos resistance in Spodoptera exigua

Hu, Bo; Ren, Miaomiao; Fan, Jianfeng; Huang, Sufang; Wang, Xia; Elzaki, Mohammed Esmail Abdalla; Bass, Chris; Palli, Subba Reddy; Su, Jianya

doi:10.1016/j.jhazmat.2020.122971

Cited by 40 publications

(22 citation statements)

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“…In addition to characterizing the role of CYP321A8 in insecticide resistance, the UAS--CYP321A8 strain was constructed according to the previous methods [52]. The transgene CYP450 was expressed by crossing with the Act5C-GAL4 strain.…”

Section: Bioassays Of Transgenic D Melanogastermentioning

confidence: 99%

“…HPLC analysis of insecticide metabolism For chlorpyrifos, cypermethrin and deltamethrin metabolism studies, the in vitro reactions were performed according to our previous approach [52]. The non-insertion microsomes (empty plasmid) were used as the controls.…”

Section: Expression Of Cyp321a8 In Sf9 Cells and Functional Assaymentioning

confidence: 99%

“…All primers are displayed in S7 Table. Sf9 cells were cultured in 24-well cell plates at 4 x 10 5 cells density. The promoter constructs and expression plasmids were transfected according to our previous approach [52]. 1 μg promoter constructs (DNA5'-R, DNA5'-S, truncations and mutated truncations) and 0.02 μg pRL-CMV were co-transfected to the cells with the help of 2 μL of FUGENE transfection reagent.…”

Section: Plos Geneticsmentioning

confidence: 99%

“…The medium for transfection was replaced with 500 μL complete medium after incubation for 4h. Finally, the cells were collected after 48 h and luciferase activities were measured [52].…”

Section: Plos Geneticsmentioning

confidence: 99%

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Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua

Huang

et al. 2021

PLoS Genet

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The evolution of insect resistance to insecticides is frequently associated with overexpression of one or more cytochrome P450 enzyme genes. Although overexpression of CYP450 genes is a well-known mechanism of insecticide resistance, the underlying regulatory mechanisms are poorly understood. Here we uncovered the mechanisms of overexpression of the P450 gene, CYP321A8 in a major pest insect, Spodoptera exigua that is resistant to multiple insecticides. CYP321A8 confers resistance to organophosphate (chlorpyrifos) and pyrethroid (cypermethrin and deltamethrin) insecticides in this insect. Constitutive upregulation of transcription factors CncC/Maf are partially responsible for upregulated expression of CYP321A8 in the resistant strain. Reporter gene assays and site-directed mutagenesis analyses demonstrated that CncC/Maf enhanced the expression of CYP321A8 by binding to specific sites in the promoter. Additional cis-regulatory elements resulting from a mutation in the CYP321A8 promoter in the resistant strain facilitates the binding of the orphan nuclear receptor, Knirps, and enhances the promoter activity. These results demonstrate that two independent mechanisms; overexpression of transcription factors and mutations in the promoter region resulting in a new cis-regulatory element that facilitates binding of the orphan nuclear receptor are involved in overexpression of CYP321A8 in insecticide-resistant S. exigua.

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Section: Bioassays Of Transgenic D Melanogastermentioning

confidence: 99%

Section: Expression Of Cyp321a8 In Sf9 Cells and Functional Assaymentioning

confidence: 99%

Section: Plos Geneticsmentioning

confidence: 99%

“…The medium for transfection was replaced with 500 μL complete medium after incubation for 4h. Finally, the cells were collected after 48 h and luciferase activities were measured [52].…”

Section: Plos Geneticsmentioning

confidence: 99%

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Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua

Huang

et al. 2021

PLoS Genet

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“…CncC is constitutively overexpressed in a Chinese field population of S. exigua resistant to chlorpyrifos and cypermethrin [94]. Several studies on this resistant strain showed that CncC is involved in controlling the expression of detoxification genes including P450s (CYP321A8, CYP321A16, and CYP332A1) [11,132] and GSTs (GSTo2, GSTe6, GSTd3) [94]. However, none of these studies mention the role of CncC in the expression of ABC transporters.…”

Section: Resistance and Regulation Of Abcs Expressionmentioning

confidence: 99%

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Hilliou

Chertemps

Maı̈bèche

et al. 2021

Insects

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The genus Spodoptera (Lepidoptera: Noctuidae) includes species that are among the most important crop pests in the world. These polyphagous species are able to feed on many plants, including corn, rice and cotton. In addition to their ability to adapt to toxic compounds produced by plants, they have developed resistance to the chemical insecticides used for their control. One of the main mechanisms developed by insects to become resistant involves detoxification enzymes. In this review, we illustrate some examples of the role of major families of detoxification enzymes such as cytochromes P450, carboxyl/cholinesterases, glutathione S-transferases (GST) and transporters such as ATP-binding cassette (ABC) transporters in insecticide resistance. We compare available data for four species, Spodoptera exigua, S. frugiperda, S. littoralis and S. litura. Molecular mechanisms underlying the involvement of these genes in resistance will be described, including the duplication of the CYP9A cluster, over-expression of GST epsilon or point mutations in acetylcholinesterase and ABCC2. This review is not intended to be exhaustive but to highlight the key roles of certain genes.

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Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Jin

Wang

et al. 2021

Pest Management Science

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BACKGROUND Nilaparvata lugens, a destructive rice pest in Asia, has developed resistance to many insecticides, including the neonicotinoid clothianidin. CYP6ER1 plays an important role in N. lugens resistant to clothianidin, but only limited information on the transcriptional regulation of CYP6ER1 overexpression in clothianidin resistance is available. RESULTS In this study, the transcription factor activator protein 1 (AP‐1) was found to be overexpressed in a clothianidin‐resistant strain of N. lugens and several field resistant populations. RNA interference‐mediated silencing of NlAP‐1 significantly decreased CYP6ER1 expression and increased the susceptibility of N. lugens to clothianidin. Additionally, NlAP‐1 was highly expressed in egg and adult stages, and in midguts, and NlAP‐1 was upregulated and induced to a greater extent in the clothianidin‐resistant strain after exposure to clothianidin. Finally, dual‐luciferase reporter assays confirmed the interaction between NlAP‐1 and the two predicted binding sites in the CYP6ER1 promoter. CONCLUSION NlAP‐1 bound the −1388 to −1208‐bp region of the CYP6ER1 promoter, enhancing its activity and then regulate the expression of CYP6ER1. These findings enhance our knowledge of the transcriptional regulation of the P450 genes that mediate insecticide resistance in insect pests. © 2021 Society of Chemical Industry.

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Xenobiotic transcription factors CncC and maf regulate expression of CYP321A16 and CYP332A1 that mediate chlorpyrifos resistance in Spodoptera exigua

Cited by 40 publications

References 50 publications

Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua

Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

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