Activator protein‐1 mediated <scp><i>CYP6ER1</i></scp> overexpression in the clothianidin resistance of <scp><i>Nilaparvata lugens</i></scp> (Stål)

Jin, Ruoheng; Wang, Yue; He, Biyan; Zhang, Yunhua; Cai, Tingwei; Wan, Hu; Jin, Byung Rae; Li, Jianhong

doi:10.1002/ps.6482

Cited by 16 publications

(18 citation statements)

References 40 publications

(45 reference statements)

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“…For instance, transcription factors CncC/Maf, CREB, HNF4 and AhR/ARNT has been respectively proved to participate in the regulation of P450 genes mediating imidacloprid susceptibility in insects (Cheng et al, 2021;Kalsi and Palli, 2017;Wang et al, 2021;Yang et al, 2020). AP-1 protein was showed to mediate clothianidin resistance by regulating CYP6ER1 in N. lugens (Jin et al, 2021). In the present study, it was found that FoxO mediated nitenpyram resistance in N. lugens, which might be through regulating CYP4CE1 expression by FoxO.…”

Section: Discussionsupporting

confidence: 50%

“…In N. lugens, AKH-mediated ROS burst induced CYP6ER1 expression by activating CncC and MafK after imidacloprid exposure (Tang et al, 2020). Additionally, activator protein 1 (AP-1) was found to be overexpressed in a clothianidin resistant strain and eld resistant populations of N. lugens, regulating the expression of CYP6ER1 to mediate clothianidin resistance (Jin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Two point mutations in CYP4CE1 promoter contributed to the differential regulation of CYP4CE1 expression by FoxO between susceptible and nitenpyram resistant Nilaparvata lugens

Zhang

Lin

Yang

et al. 2023

Preprint

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Four P450s were reported to be important for imidacloprid resistance in Nilaparvata lugens, a major insect pest on rice, which was confirmed in this study in an imidacloprid resistant strain (ImiR). Here we found that only two (CYP4CE1 and CYP6ER1) from these four P450 genes were over-expressed in a nitenpyram resistant strain (NitR) when compared to a susceptible strain SUS. CYP4CE1 RNAi reduced nitenpyram and imidacloprid resistance in NitR and ImiR strains, with a more reduction in nitenpyram resistance. The transcription factor FoxO mediated nitenpyram resistance in NitR and ImiR strains, but it was not differentially expressed among strains. The potential reason for the differential regulation of FoxO on CYP4CE1 expression was mainly from sequence differences in CYP4CE1promoter between susceptible and resistant insects. In six FoxO response elements predicted in CYP4CE1 promoter, the single-nucleotide polymorphisms were frequently detected in over 50% of NitR and ImiR individuals. The luciferase reporter assays showed that two mutations, -650T/G and -2205T/A in two response elements at the position of -648 bp and -2200 bp, mainly contributed to the enhanced regulation on CYP4CE1 expression by FoxO in resistant insects. The frequency was over 69% for both -650T/G and -2205T/A detected in NitR and ImiR individuals, but less than 20% in SUS insects. In conclusion,CYP4CE1 over-expression importantly contributed to nitenpyram resistance in N. lugens, and two mutations in CYP4CE1 promoter of resistant insects led to an enhanced regulation on CYP4CE1 expression by FoxO.

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Section: Discussionsupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Two point mutations in CYP4CE1 promoter contributed to the differential regulation of CYP4CE1 expression by FoxO between susceptible and nitenpyram resistant Nilaparvata lugens

Zhang

Lin

Yang

et al. 2023

Preprint

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“…It is also important to note that, while the data presented in the current work provide valuable information on deregulated transcripts in response to insecticides in L. decemlineata not previously exposed to various compounds, subsequent efforts are warranted to better delineate the underlying roles, if any, of these targets in the process of insecticide resistance. Characterizing the expression status of these transcripts in L. decemlineata populations with confirmed resistance towards a given compound of interest such as clothianidin, similar to work conducted on the potential role of CYP6er1 in clothianidin-resistant populations of N. lugens [ 39 ], would be an interesting starting point to better position these targets within the context of insecticide resistance in this potato pest. It is finally important to point out that leveraging, in the mid- to long-term, a high-throughput sequencing analysis approach to decipher a plethora of deregulated transcripts, besides the select group assessed in the current work, would yield valuable information and should be considered.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Insecticide Response-Associated Transcripts in the Colorado Potato Beetle: Relevance of Selected Cytochrome P450s and Clothianidin

Bouafoura

Bastarache

Ouédraogo

et al. 2022

Insects

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is known for its capacity to cause significant damages to potato crops worldwide. Multiple approaches have been considered to limit its spread including the use of a diverse arsenal of insecticides. Unfortunately, this insect frequently develops resistance towards these compounds. Investigating the molecular bases underlying the response of L. decemlineata against insecticides is of strong interest to ultimately devise novel and targeted approaches aimed at this pest. This work aimed to characterize, via qRT-PCR, the expression status of targets with relevance to insecticide response, including ones coding for cytochrome P450s, glutathione s-transferases, and cuticular proteins, in L. decemlineata exposed to four insecticides; chlorantraniliprole, clothianidin, imidacloprid, and spinosad. Modulation of levels associated with transcripts coding for selected cytochrome P450s was reported in insects treated with three of the four insecticides studied. Clothianidin treatment yielded the most variations in transcript levels, leading to significant changes in transcripts coding for CYP4c1, CYP4g15, CYP6a13, CYP9e2, GST, and GST-1-Like. Injection of dsRNA targeting CYP4c1 and CYP9e2 was associated with a substantial decrease in expression levels and was, in the case of the latter target, linked to a greater susceptibility of L. decemlineata towards this neonicotinoid, supporting a potential role for this target in clothianidin response. Overall, this data further highlights the differential expression of transcripts with potential relevance in insecticide response, as well as generating specific targets that warrant investigation as novel dsRNA-based approaches are developed against this insect pest.

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“…42 For example, clothianidin treatment enhanced the expression of CYP6ER1in N. lugens through the transcription factor activator protein 1 (NlAP-1). 43 In Bemisia tabaci, the upregulation of CYP6CM1 by imidacloprid was through the mitogen-activated protein kinase (MAPK)-directed activator transcription factor CREB (cAMP-response element binding protein). 44 So, we supposed that the effects of flonicamid treatment on salivary protein gene expressions were through some important transcription factors, which need further identification and verification in the future.…”

Section: Discussionmentioning

confidence: 99%

“…For example, clothianidin treatment enhanced the expression of CYP6ER1 in N. lugens through the transcription factor activator protein 1 ( NlAP-1 ) . In Bemisia tabaci, the upregulation of CYP6CM1 by imidacloprid was through the mitogen-activated protein kinase (MAPK)-directed activator transcription factor CREB (cAMP-response element binding protein) .…”

Section: Discussionmentioning

confidence: 99%

Reducing Expression of Salivary Protein Genes by Flonicamid Partially Contributed to Its Feeding Inhibition of the Brown Planthopper on Rice

Gao

Yuan

Lin

et al. 2023

J. Agric. Food Chem.

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Flonicamid inhibits the feeding of piercing−sucking pests as a selective systemic insecticide. The brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most serious pests on rice. During feeding, it uses its stylet to collect sap by penetrating the phloem, and at the same time, it delivers saliva into the rice plant. Insect salivary proteins play important roles in feeding and interacting with plants. Whether flonicamid affects the expression of salivary protein genes and then inhibits the feeding of BPH is not clear. Here, from 20 functionally characterized salivary proteins, we screened five salivary proteins (NlShp, NlAnnix5, Nl16, Nl32, and NlSP7) whose gene expressions were significantly inhibited by flonicamid. We performed experimental analysis on two of them (Nl16 and Nl32). RNA interference of Nl32 significantly reduced the survival rate of BPH. Electrical penetration graph (EPG) experiments showed that both flonicamid treatment and knockdown of Nl16 and Nl32 genes significantly reduced the feeding activity of N. lugens in the phloem and also reduced the honeydew excretion and fecundity. These results suggested that the inhibition of flonicamid on the feeding behavior in N. lugens might be partially attributed to its effect on the expression of salivary protein genes. This study provides a new insight into the mechanism of action of flonicamid on insect pests.

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Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Cited by 16 publications

References 40 publications

Two point mutations in CYP4CE1 promoter contributed to the differential regulation of CYP4CE1 expression by FoxO between susceptible and nitenpyram resistant Nilaparvata lugens

Two point mutations in CYP4CE1 promoter contributed to the differential regulation of CYP4CE1 expression by FoxO between susceptible and nitenpyram resistant Nilaparvata lugens

Characterization of Insecticide Response-Associated Transcripts in the Colorado Potato Beetle: Relevance of Selected Cytochrome P450s and Clothianidin

Reducing Expression of Salivary Protein Genes by Flonicamid Partially Contributed to Its Feeding Inhibition of the Brown Planthopper on Rice

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