Xenobiotic Response Elements (XREs) from Human CYP1A1 Gene Enhance the hTERT Promoter Activity

Шепелев, М. В.; Калиниченко, С. В.; Саакян, Е.К.; Коробко, И. В.

doi:10.1134/s1607672919020200

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…hTERT can significantly inhibit the growth of carcinoma cells, which exist in multiple organisms. hTERT expression is inhibited in normal tissues, but not in cancer cells ( 15 , 19 ).…”

Section: Discussionmentioning

confidence: 99%

“…When the expression of all samples all went through GAPDH level correction, according to the 2 -ΔΔCt formula, the relative expression level of samples was calculated. hTERT primer sequence in upstream was 5'-GGCCTTCACCACCAGCGTGC-3', and in downstream was 5'-AGGACCCCTGCCCAACGGGC-3', with amplification fragment 427 bp; within the referring to the sequence of GAPDH primers, upstream was 5'-AGGTAGGACGAGGTCGGAGTC-3', and downstream was 5'-GAATTAGGTACTAGGATTTC-3' ( 14 , 15 ).…”

Section: Methodsmentioning

confidence: 99%

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Downregulation of hTERT contributes to ovarian cancer apoptosis and inhibits proliferation of ovarian cancer cells

Wang¹,

Liang²,

Yang³

2020

Transl Cancer Res TCR

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Background: Our study aims to study the effects of the exogenous human telomerase reverse transcriptase (hTERT) interfering gene on the ovarian cancer cell line SKOV3 through proliferation and apoptosis.Methods: Lipofectamine TM2000 was used to transfer the hTERT interfering gene into the SKOV3 cells.After a predetermined amount of time after transfection with the hTERT interfering genes, the expression of the tumor-related genes was detected using real-time quantitative polymerase chain reaction (RT-qPCR), and relative protein level was detected using western blot analysis. Cell morphology was acquired by microscopy. Cell viability was detected by middle-time-spray (MTS), and cell cycle and apoptosis were detected by flow cytometry.Results: Forty-eight hours after transfection, the expression of tumor-related proteins in the experimental group was increased compared with the control group, and the difference was statistically significant (P<0.05).The cell morphology showed a significant difference between the control group and the hTERT shRNA group. Furthermore, 48 and 72 h after transfection with the hTERT interfering gene, the cell viability inhibition rates of the hTERT shRNA group were 0.77±0.02 and 0.88±0.01 respectively. Compared with the control group, the cell viability inhibition rates were 11.97±2.37 (%) and 18.72±1.01 (%), respectively, with statistically significant differences (P=0.009, P=0.004). Flow cytometry detected the apoptosis peak of SKOV3 cells in the experimental group 48 h after transfection with the hTERT interfering gene. Propidium iodide (PI) and Annexin V-FITC revealed that the apoptotic cells accounted for 18.13% of the total number, which was significantly higher than the 3.85% demonstrated by the control group. Conclusions:The amount and activity of SKOV3 cells in ovarian cancer was decreased after the exogenous introduction of the hTERT interfering gene.

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“…hTERT can significantly inhibit the growth of carcinoma cells, which exist in multiple organisms. hTERT expression is inhibited in normal tissues, but not in cancer cells ( 15 , 19 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Downregulation of hTERT contributes to ovarian cancer apoptosis and inhibits proliferation of ovarian cancer cells

Wang¹,

Liang²,

Yang³

2020

Transl Cancer Res TCR

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“…The suicide gene system driven by hTERTp is highly specific to thyroid cancer cells, 90 although the driving efficiency in cancer cells is relatively low. 91 Chang et al 92 which are labeled with 131 I by using chloramine T. Under a weak radiation of 131 I, E9 significantly upregulating suicide genes by enhancing the activity of hTERTp. Besides, 131 I also has a certain killing effect after entering the host cells.…”

Section: Targeting Nuclear Acidsmentioning

confidence: 99%

Current Application of Nanoparticle Drug Delivery Systems to the Treatment of Anaplastic Thyroid Carcinomas

Wang,

Zhang

2023

IJN

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Anaplastic thyroid carcinomas (ATCs) are a rare subtype of thyroid cancers with a low incidence but extremely high invasiveness and fatality. The treatment of ATCs is very challenging, and currently, a comprehensive individualized therapeutic strategy involving surgery, radiotherapy (RT), chemotherapy, BRAF/MEK inhibitors (BRAFi/MEKi) and immunotherapy is preferred. For ATC patients in stage IVA/IVB, a surgery-based comprehensive strategy may provide survival benefits. Unfortunately, ATC patients in IVC stage barely get benefits from the current treatment. Recently, nanoparticle delivery of siRNAs, targeted drugs, cytotoxic drugs, photosensitizers and other agents is considered as a promising anti-cancer treatment. Nanoparticle drug delivery systems have been mainly explored in the treatment of differentiated thyroid cancer (DTC). With the rapid development of drug delivery techniques and nanomaterials, using hybrid nanoparticles as the drug carrier to deliver siRNAs, targeted drugs, immune drugs, chemotherapy drugs and phototherapy drugs to ATC patients have become a hot research field. This review aims to describe latest findings of nanoparticle drug delivery systems in the treatment of ATCs, thus providing references for the further analyses.

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“…Evidence indicated that the suicide gene system driven by hTERTp shows specificity toward thyroid cancer cells [ 12 ]. However, hTERTp exhibits a weak driving efficiency in cancer cells [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Chang

Ling

et al. 2021

Bioengineered

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Differentiated thyroid cancer (DTC), such as papillary thyroid cancer, has a good prognosis after routine treatment. However, in the course of treatment, 5% to 20% of cases may dedifferentiate and can be transformed into dedifferentiated DTC (deDTC) or anaplastic thyroid cancer, leading to treatment failure. To date, several drugs have been used effectively for dedifferentiated thyroid cancer, whereas gene therapy may be a potential method. Literature reported that double suicide genes driven by human telomerase reverse transcriptase promoter (hTERTp) can specifically express in cancer cells and kill them. However, the weak activity of hTERTp limits its further research. To overcome this weakness, we constructed a novel chitosan nanocarrier containing double suicide genes driven by a 'gene switch' (a cascade of radiation enhancer E9 and a hTERTp). The vector was labeled with iodine-131 ( 131 I). On one hand, E9 can significantly enhance the activity of hTERTp under the weak radiation of 131 I, thereby increasing the expression of double suicide genes in deDTC cells. On the other hand, 131 I also plays a certain killing role when it enters host cells. The proposed nanocarrier has good specificity for deDTC cells and thus deserves further study.

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Xenobiotic Response Elements (XREs) from Human CYP1A1 Gene Enhance the hTERT Promoter Activity

Cited by 4 publications

References 9 publications

Downregulation of hTERT contributes to ovarian cancer apoptosis and inhibits proliferation of ovarian cancer cells

Downregulation of hTERT contributes to ovarian cancer apoptosis and inhibits proliferation of ovarian cancer cells

Current Application of Nanoparticle Drug Delivery Systems to the Treatment of Anaplastic Thyroid Carcinomas

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

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