Xanthohumol inhibits cell cycle progression and proliferation of larynx cancer cells in vitro

Xanthohumol inhibits the extracellular signal regulated kinase (ERK) signalling pathway and suppresses cell growth of lung adenocarcinoma cells

“…The results of our present studies revealed that the XN treatment caused concentration-dependent suppression of ERK1/2 phosphorylation in A549 cells. This is in agreement with our previous investigation on larynx cancer cells (Sławi nska-Brych et al, 2015) but inconsistent with the published data on glioma (Festa et al, 2011) and leukemic cells , which demonstrated strong induction of ERK activation by this compound. These discrepancies could be determined by the specific genotype/phenotype of the studied cells and require further studies.…”

“…the most abundant prenylflavonoid in the Humulus lupulus L. hop cones, seems to be very attractive. In experimental studies, it has been demonstrated that XN has a wide array of pharmacological and chemopreventive properties (Albini et al, 2006), including inhibition of cell proliferation and induction of programmed cell death (apoptosis and/or autophagy) in different types of human malignancies including gliomas, leukemia, breast, ovarian, colon, and prostate carcinomas (Benelli et al, 2012;Festa et al, 2011;Miranda et al, 1999;Drenzek et al, 2011;Delmulle et al, 2008), as well as in laryngeal carcinoma, as demonstrated in our recent study (Sławi nska-Brych et al, 2015). The cellular effects of XN are mainly related to inhibition of DNA synthesis, inhibition of topoisomerase I, a decrease in cyclooxygenase and aromatase activity, blockade of cell cycle progression or induction of terminal differentiation resulting in attenuation of the metastatic process, and inhibition of cell motility and tumor invasion (Albini et al, 2006;Vanhoecke et al, 2005;Benelli et al, 2012;Araújo et al, 2011).…”

“…The anticancer mode of action of XN was attributed to the cell cycle arrest and initiation of both caspase-dependent and caspase-independent apoptosis in different types of cancer cells (Miranda et al, 1999;Colgate et al, 2007;Delmulle et al, 2008;Festa et al, 2011). In our recently published study on the RK33 larynx cancer cell line (Sławi nska-Brych et al, 2015), blockade of cell cycle progression (at G1 phase) was achieved by XN-modulated expression of cell cycle-related proteins (such as cyclin D1, p21, p53) and molecules involved in apoptotic cell death (caspases À3,8,9,. The same mode of XN action was found in the present study, when lung cancer cells were subjected to the XN treatment-elevated p21 and p53 mRNA levels and down-regulation of cyclin D1 mRNA expression.…”

Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549

“…The PI fluorescence intensity of individual nuclei was determined and at least 10,000 events were measured within an acquisition rate >60 events/sec. Cell cycle analyses were performed with the use of software CellQuest Pro Version 6.0. for Macintosh operating system [25].…”

Insecticidal effects of extracts of Humulus lupulus (hops) L. cones and its principal component, xanthohumol

“…1). The xanthohumol has inhibition activity against some *Author for correspondence Phone: +90 (472) 2159863 Fax: +90 (472) 2151182 E-mail: aydintuba25@gmail.com carcinogenesis (Zanoli & Zavatti, 2008;Sławinska-Brych et al, 2015), anti-infective activity against Gram-positive bacteria, viruses, fungi and malarial protozoa (Gerhäuser, 2005;Zanoli & Zavatti, 2008). It has also antioxidant, antiviral and antitumoral activities, including liver, breast, colon, ovarian and prostate (Venè et al, 2012;Yoshimaru et al, 2014;Zhao et al, 2016).…”