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Pinheiro, Sandra Maria Botelho; Chaves, M. E. C.

doi:10.1023/a:1008963202258

Cited by 8 publications

(1 citation statement)

References 8 publications

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“…In our laboratories, several supports have been proposed for biomolecules immobilization: Dacron [42,43]; ferromagnetic Dacron [44,45]; polyvinyl alcohol-glutaraldehyde network [46][47][48]; polyaniline-Dacron composite [49]; plasticized filter paper with polyvinyl alcohol-glutaraldehyde [50].…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

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This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane-polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.

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Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

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Investigation on the chemiluminescence reaction of the phenylhydrazine‐luminol–peroxide system

Chandel¹,

Khan²,

Kher³

et al. 2012

Luminescence

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We studied the chemiluminescence (CL) oxidation of phenyl hydrazine-luminol with various organic and inorganic peroxides. Maximum CL intensity for this system was obtained for t-butylhydroperoxide. The enhancement in CL depended strongly on pH and was greatest at pH 12.5. The solvent drastically enhanced the CL intensity. DMSO was found to increase the CL intensity many-fold as compared to acetonitrile and water. The effect of temperature on CL intensity has also been studied. The CL spectra revealed a broad peak at 425 nm, which suggests excited 3-aminophthalate ion as the luminophor. A mechanism to explain the reactions is suggested.

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A new strategy for photoelectrochemical DNA biosensor using chemiluminescence reaction as light source

Zhang

Zhao

Zhou

et al. 2011

Biosensors and Bioelectronics

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Untitled

Cited by 8 publications

References 8 publications

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Investigation on the chemiluminescence reaction of the phenylhydrazine‐luminol–peroxide system

A new strategy for photoelectrochemical DNA biosensor using chemiluminescence reaction as light source

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