X-ray radiation of poly-L-arginine hydrochloride and multilayered DNA-coatings

Stypczyńska, Agnieszka; Nixon, Tony; Mason, Nigel J.

doi:10.1140/epjd/e2014-40845-8

Cited by 16 publications

(17 citation statements)

References 31 publications

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“…The X‐Ray Photoelectron Spectoscopy (XPS) high‐resolution spectra recorded on grafted catheters incubated for 24 h were used to assess precise attributions of atom environments. The C1s region was fitted with four components while the N1s region was decomposed into two contributions ( Figure ), in agreement with previous studies of arginine‐containing peptide (details of component assignments are given in Supporting Information) . These observations prompted us to choose the N1s element as a marker to quantify the amount of molecules grafted on the silicone surface (see Supporting Information for detailed equations).…”

Section: Antibacterial Silicone Cathetersupporting

confidence: 75%

Simple and Specific Grafting of Antibacterial Peptides on Silicone Catheters

Pinese

Jebors

Echalier

et al. 2016

Adv Healthcare Materials

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To fight against nosocomial infection initiated by colonization of medical devices, a strategy enabling the direct and fast functionalization of silicone surfaces is proposed. This strategy proceeds in a site-specific way using original hybrid silylated antibacterial peptides. This safe and up-scalable method guarantees a covalent and robust immobilization with the correct orientation of the bioactive moiety. Importantly it also avoids multi-step chemical modifications of the surface or multi-layer polymer coatings. As proof of concept, antibacterial silicone catheter has been prepared whose immediate and long term efficiency is superior by comparison to similar silver-embedded materials.

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Section: Antibacterial Silicone Cathetersupporting

confidence: 75%

Simple and Specific Grafting of Antibacterial Peptides on Silicone Catheters

Pinese

Jebors

Echalier

et al. 2016

Adv Healthcare Materials

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“…Although to the best of our knowledge, XPS studies of X-ray or ion induced damage of temperas have not been published, X-ray induced damage of amino acids and DNA has attracted some attention due to the biological importance of these compounds [46][47][48][49]. The downward trend in O 1s and N 1s intensities with respect to the one of C 1s in the present work has also been observed in X-ray irradiation of DNA [46,47] and amino acids through XPS analysis [48,49]. In the case of DNA, dehydrogenation, oxygen loss and radical formation was deduced from XPS data.…”

Section: Azurite Egg Temperamentioning

confidence: 99%

X-ray and ion irradiation effects on azurite, malachite and alizarin pictorial models

Carrasco

Oujja

Sanz

et al. 2018

Microchemical Journal

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The analysis by intense radiation sources of painting materials used in artworks gives valuable information about their composition and state of conservation, but it can also induce undesired modifications on the studied substrates. Here we use X-ray photoelectron spectroscopy (XPS) to follow the surface chemical changes that prolonged irradiation with soft X-rays (with maximum doses ranging from ~ 1.2x10 17 to ~ 3.4x10 17 photons cm -2 ) produce on azurite, malachite and alizarin pigments and on two egg-yolk temperas containing azurite or alizarin. The effects following exposure to Argon ion irradiation have been also studied for malachite and azurite tempera. XPS spectra of the samples show that both types of radiation induce similar superficial alterations, although, at the selected experimental conditions, the modification rate is slower for exposure to soft X-rays. The highest radiation stability is shown for alizarin and, on the contrary, azurite and malachite experience a progressive chemical reduction of Cu 2+ to Cu + . The reduction of copper takes also place in the azurite tempera, while the proteins and lipids of the egg-yolk binder of azurite and alizarin temperas are subjected to degradation by irradiation with photons and ions. By employing XPS, the cleavage of C-N, C-O(H) and C=O bonds has been identified in the egg-yolk temperas.

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“…The XPS wide-range scan spectra of neat Fe 3 O 4 , DE, and Arg-mag-DE NPs are depicted in Figure . The XPS total survey measurements recorded a binding energy in the 0–1000 eV range and clearly showed dominating peaks at 76.58 (Al 2p), 105.03 (Si 2p), 284.08 (C 1s), 535.12 (O 1s), and 714.88 (Fe 2p) eV for DE (Figure A, line i) , and 103.68 (Si 2p), 285.03 (C 1s), 401.08 (N 1s), 532.08 (O 1s), and 711.18 (Fe 2p) eV for Arg-mag-DE (Figure A, line ii). , However, according to the wide-range scan, the presence of N 1s and the disappearance of Al 2p Arg-mag-DE NPs clearly show that Fe 3 O 4 coated the surface of DE and that l -arginine was attached to the surface of Fe 3 O 4 . Figure B–G (line ii) represents the deconvoluted high-resolution XPS spectra for Si 2p, C 1s, N 1s, O 1s, and Fe 2p of DE and mag-DE NPs, respectively.…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis-Based Magnetic Diatoms for Biological Applications

Dao

Ankireddy

Zhao

et al. 2021

ACS Sustainable Chem. Eng.

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Here, we report the green synthesis of silica-based materials for the treatment of biological samples such as bacteria enrichment and exosome isolation. The synthesized materials were nanosized, recyclable, and magnetically separable using an eco-friendly hydrothermal synthesis approach. Fe 3 O 4 formation on the surface of biosilica porous diatomite via precipitation was used to create a magnetic diatom (mag-DE) through a bottom-up approach, which was low cost, robust, nontoxic, and eco-friendly. Following the addition of L-arginine-containing amino groups, the mag-DE showed broad absorbance in the visible light spectrum (UV−vis), and the amine functional groups of 3-aminopropyl triethoxysilane on the DE via the amidine bond were confirmed using Fourier transform infrared analysis. Further studies were conducted by conjugating the mag-DE with concanavalin A (ConA), a known micro-antimicrobial agent for bacterial enrichment, as well as with a specific antibody for exosome isolation. The large-volume samples obtained were easy to use, and the materials could enrich a minimum of 50 colony-forming units (CFUs) of Salmonella and also showed a promising ability to capture another bacterial species. In addition, the modified synthetic mag-DE could be used for exosome isolation using affinity purification. Functionalized mag-DE could be magnetically isolated in a rapid and more efficient manner than using the conventional method, which can be altered for exosome isolation in research and clinical applications. This study presents the unique physical and chemical properties of green synthesized mag-DE, whose surface can be easily modified. Thus, it creates a novel potential strategy for several desirable applications for various types of biological samples.

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X-ray radiation of poly-L-arginine hydrochloride and multilayered DNA-coatings

Cited by 16 publications

References 31 publications

Simple and Specific Grafting of Antibacterial Peptides on Silicone Catheters

Simple and Specific Grafting of Antibacterial Peptides on Silicone Catheters

X-ray and ion irradiation effects on azurite, malachite and alizarin pictorial models

Green Synthesis-Based Magnetic Diatoms for Biological Applications

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