Simple and Specific Grafting of Antibacterial Peptides on Silicone Catheters

Pinese, Coline; Jebors, Said; Echalier, Cécile; Licznar-Fajardo, Patricia; Garric, Xavier; Humblot, Vincent; Calers, Christophe; Martinez, Jean; Mehdi, Ahmad; Subra, Gilles

doi:10.1002/adhm.201600757

Cited by 45 publications

(27 citation statements)

References 27 publications

(26 reference statements)

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“…This value of ~1 active molecule per nm² is within the range usually observed in several systems. For instance, a small arginine branched tripeptide grafted on silicone average 0.2 peptide/nm² [42] while and α-helical peptide on gold rises up to 1 molecule/nm² [37].…”

Section: Resultsmentioning

confidence: 99%

Engineering of Antimicrobial Surfaces by Using Temporin Analogs to Tune the Biocidal/antiadhesive Effect

et al. 2019

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Proliferation of resistant bacteria on biomaterials is a major problem leading to nosocomial infections. Due to their broad-spectrum activity and their ability to disrupt bacterial membranes through a rapid membranolytic mechanism, antimicrobial peptides (AMPs) are less susceptible to the development of bacterial resistance and therefore represent good candidates for surface coating strategies to prevent biofilm formation. In this study, we report on the covalent immobilization of temporin-SHa, a small hydrophobic and low cationic antimicrobial peptide exhibiting broad-spectrum activity, and (SHa) analogs on modified gold surfaces. Several analogs derived from SHa with either a carboxamidated ([K3]SHa, d-[K3]SHa) or a carboxylated C-terminus ([K3]SHa-COOH) were used to achieve peptide grafting on gold surfaces modified by a thiolated self-assembled monolayer (SAM). Surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy (PM-RAIRS) and X-ray photoemission spectroscopy (XPS). The antibacterial properties of the temporin-functionalized surfaces were tested against the Gram-positive Listeria ivanovii. Direct visualization of the peptide effects on the bacterial membrane was investigated by scanning electron microscopy equipped with a field emission gun (SEM-FEG). All active temporin analogs were successfully grafted and display significant antibacterial activity (from 80 to 90% killing efficiency) in addition to a 2-fold decrease of bacterial adhesion when all d-SHa analogs were used.

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Section: Resultsmentioning

confidence: 99%

Engineering of Antimicrobial Surfaces by Using Temporin Analogs to Tune the Biocidal/antiadhesive Effect

et al. 2019

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“…Recently, Pinese et al introduced a site‐specific silylated AMP, which can directly modify silicone catheter and greatly simplify the conventional catheter modification operations (Fig. ) …”

Section: Antimicrobial Strategies For Urinary Cathetersmentioning

confidence: 99%

“…8). 78 Overall, great progresses have been achieved in the development of AMP-impregnated catheters. The implant coating of AMPs is still challenged by a series of factors, including loss of antimicrobial activities, nonspecific binding, altered peptide orientations, inadequate surface AMP density, nonoptimal coating, and high pH sensitivity.…”

Section: Bactericidal Coatingsmentioning

confidence: 99%

Antimicrobial strategies for urinary catheters

Zhu

Wang

et al. 2018

J Biomedical Materials Res

104

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Over 75% of hospital‐acquired or nosocomial urinary tract infections are initiated by urinary catheters, which are used during the treatment of 16% of hospitalized patients. Taking the United States as an example, the costs of catheter‐associated urinary tract infections (CAUTI) are in excess of $451 million dollars/year. The biofilm formation by pathogenic microbes that protects pathogens from host immune defense and antimicrobial agents is the leading cause for CAUTI. Thus, tremendous efforts have been devoted to antimicrobial coating for urinary catheters in the past few decades, and it has been demonstrated to be one of the most direct and efficient strategies to reduce infections. In this article, we briefly summarize the current methods for preparation of antimicrobial coatings based on different stages in the biofilm formation, highlight recent progress in the urinary catheter coating material design and selection, discuss approaches to improving their long‐term antimicrobial efficacy, biocompatibility, multidrug resistance and recurrent infections, and finally outline future requirements and prospects in antimicrobial coating material design. The scope of the works surveyed is confined to antimicrobial urinary catheters. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 445–467, 2019.

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“…Interestingly, any type of peptides but also biopolymers and dyes can be silylated and thus can participate to the covalent formation of the material. We already used this sol-gel process to prepare peptide-functionalized fluorescent silica nanoparticles [10,11], grafting of wound-healing or antibacterial peptides on silicone catheter, [12] dressings [13] and titanium [14] and comb-like and silicone linear polymers were prepared. [15,16] At pH 7, the sol gel process needs to be catalyzed with a nucleophilic compound to faster the gelation.…”

Section: Figure 1: Hybrid Hydrogel Compositionmentioning

confidence: 99%