X-ray photoelectron and infrared spectroscopy of glycine adsorbed upon copper

Uvdal, Kajsa; Bodö, P.; Ihs, A.; Liedberg, Bo; Salaneck, W. R.

doi:10.1016/0021-9797(90)90336-m

Cited by 47 publications

(47 citation statements)

References 31 publications

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“…The second one, at 286.2 ± 0.1 eV, is assigned to carbon in C-N and C-S bonds, the third one at 287.7 ± 0.1 eV to carbon of amide functions and the highest BE peak, at 289.1 ± 0.1 eV to carbon of COOH function of the acidic or carboxylate groups for the basic or zwiterrionic form of GSH. [5,15,16] The N 1s peak presents two contributions for both the acidic and zwiterrionic forms, at 399.7 ± 0.1 and 401.3 ± 0.1 eV respectively, attributed to nitrogen of amide groups and NH 3 + [2,17] ; note that the intensity ratio NH 3 + /O C-NH = 1/2 is in agreement with the molecular formula of GSH. For the anionic form, there is only one contribution at 399.7 ± 0.1 eV assigned to nitrogen in NH 2 and in the amide functions (O C-NH).…”

Section: Xps Analysissupporting

confidence: 65%

Glutathione adsorption from UHV to the liquid phase at various pH on gold and subsequent modification of protein interaction

Vallée

Humblot

Méthivier

et al. 2008

Surface & Interface Analysis

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This study is part of a project aiming at a better understanding of polypeptide and protein interactions with a metal surface. A tripeptide (the glutathione, or GSH) was deposited, under ultra high vacuum, on Au (111). From reflection-absorption infrared spectra (RAIRS), GSH was adsorbed in its zwiterrionic form which corresponds to the solid state one. GSH was also adsorbed on Au (111) surfaces from solutions at different pH values. These GSH so-formed monolayers were then tested to anchor a well-known protein, the bovine serum albumin (BSA); an effect of the structure and charge of the GSH layers on the protein adsorptions was made clear.The combination of XPS and polarisation-modulation (PM)-RAIRS results showed that the adsorbed GSH is in an identical form as that in solution (ionic state and conformation). When BSA is adsorbed from PBS solutions on the various layers of GSH the anionic or cationic nature of the GSH layer does not influence the adsorption of protein. On the other hand, adsorption of BSA in pure water is strongly favoured on cationic layers.

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Section: Xps Analysissupporting

confidence: 65%

Glutathione adsorption from UHV to the liquid phase at various pH on gold and subsequent modification of protein interaction

Vallée

Humblot

Méthivier

et al. 2008

Surface & Interface Analysis

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“…To confirm the obtained absorption data and perform band assignments, the glycine spectra were compared with spectra of glycine in KBr pellets as measured by Ihs et al (1990), Uvdal et al (1990) and Rosado et al (1998). The D-alanine spectra were compared with spectra of L-alanine in KBr pellets determined by Fischer (1999, 2000) and Rozenberg et al (2003).…”

Section: Resultsmentioning

confidence: 99%

Amino acid photostability on the Martian surface

Kate

Garry

Peeters

et al. 2005

Meteoritics &Amp; Planetary Science

128

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Abstract-In the framework of international planetary exploration programs, several space missions are planned to search for organics and bio-signatures on Mars. Previous attempts have not detected any organic compounds in the Martian regolith. It is therefore critical to investigate the processes that may affect organic molecules on and below the planet's surface. Laboratory simulations can provide useful data about the reaction pathways of organic material at Mars' surface. We have studied the stability of amino acid thin films against ultraviolet (UV) irradiation and use those data to predict the survival time of these compounds on and in the Martian regolith. We show that thin films of glycine and D-alanine are expected to have half-lives of 22 ± 5 hr and of 3 ± 1 hr, respectively, when irradiated with Mars-like UV flux levels. Modelling shows that the half-lives of the amino acids are extended to the order of 10 7 years when embedded in regolith. These data suggest that subsurface sampling must be a key component of future missions to Mars dedicated to organic detection.

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“…Uvdal et al (1990) reported that in a thin layer of Gly on a Cu substrate, the NH3+-stretching vibration of zwitterionic Gly shifted to higher frequency, from 3169 to 3180 cm-L Jang and Condrate (1972) also observed that the NH3+-stretching band occurred at 3220 cm i in the Hmontmorillonite-Gly intercalate and concluded that the intercalated Gly molecules involved less intermolecu- A similar mechanism is assumed for the KH-amino acid intercalates. Amino acid in the crystalline (zwitterionic) state forms a strong NH3 + -.. CO0-intermolecular hydrogen bond between adjacent molecules.…”

Section: Discussionmentioning

confidence: 97%

“…In Figure 5A, trace a, the broad and strong absorption band between 3300-2300 cm -1 occurs in most amino acids in the crystalline (zwitterionic) state and is characteristic of the intermolecular hydrogen-bonding, COO-... NH3 § (Uvdal et al, 1990). In the Gly intercalate, several of these strong bands were shifted to 3300-2800 cm -~ ( Figure 5A, trace e).…”

Section: Intercalation Of Amino Acidsmentioning

confidence: 95%

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Preparation of Kaolinite-Amino Acid Intercalates Derived from Hydrated Kaolinite

Sato

1999

Clays and clay miner.

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Abstract---Intercalation of amino acids into 10.0-A hydrated kaolinite was studied by powder X-ray diffraction (XRD), differential thermal analysis-thermal gravimetry (DTA-TG), and infrared (IR) spectroscopy. Intercalation was found to be dependent on the chain-length, pH, and the concentration of the amino acid zwitterion. Near the isoelectric point, fully intercalated phases were obtained in solutions of concentration >0.5-1 M for glycine (Gly), 2-3 M for 13-alanine ({3-Ala), and 12 M for both ",/-aminobutyric acid ('y-Aba) and g-aminovaleric acid (g-Ava). e-aminocaproic acid (e-Aca) with a long chain (C = 6) was only partially intercalated. Intercalated amino acid formed a mono-molecular arrangement with the alkyl chain tilting toward the layer at an angle related to H20 content. The compositions of the intercalates of the Gly and [3-Ala are AlzSi2Os(OH)4-(Gly)0.67.0.24H20 and AIzSi2Os(OH)4.([~-Ala)0.63.0.25H20, respectively, based on TG data. From IR data, Gly and [3-Ala molecules are found intercalated as zwitterions and these molecules form hydrogen bonds with both the A1-OH and Si-O surfaces of kaolinite. Washing the intercalate with water produced a hydrated kaolinite, which may form a second amino-acid intercalate of high order. Thus, hydrated kaolinite intercalates or deintercalates amino acids depending on concentration and conditions.

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X-ray photoelectron and infrared spectroscopy of glycine adsorbed upon copper

Cited by 47 publications

References 31 publications

Glutathione adsorption from UHV to the liquid phase at various pH on gold and subsequent modification of protein interaction

Glutathione adsorption from UHV to the liquid phase at various pH on gold and subsequent modification of protein interaction

Amino acid photostability on the Martian surface

Preparation of Kaolinite-Amino Acid Intercalates Derived from Hydrated Kaolinite

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