X-ray luminescence imaging for small animals

Lun, Michael C.; Cong, Wenxiang; Arifuzzaman, Md; Ranasinghe, Meenakshi; Bhattacharya, Sriparna; Anker, Jeffery; Wang, Ge; Li, Changqing

doi:10.1117/12.2544601

Cited by 5 publications

(6 citation statements)

References 24 publications

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“… 28 X-ray luminescence nanophosphors with low emission intensity are used in X-ray luminescence computer tomography (XLCT) and focused X-ray luminescence tomography (FXLT). 11 …”

Section: Resultsmentioning

confidence: 99%

X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb

et al. 2021

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“… 28 X-ray luminescence nanophosphors with low emission intensity are used in X-ray luminescence computer tomography (XLCT) and focused X-ray luminescence tomography (FXLT). 11 …”

Section: Resultsmentioning

confidence: 99%

X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb

et al. 2021

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“…We anticipate that we are still able to demonstrate even higher-resolution imaging (less than 150 µm EtE) with this system, however, smaller targets are not possible to fabricate with our current resources. With our future small-animal dedicated focused x-ray luminescence tomography (FXLT) imaging system described in [15][16], we will have even higher spatialresolution capabilities for XLCT imaging since the x-ray beam size is approximately onehalf the size of the beam used in this study.…”

Section: Discussionmentioning

confidence: 99%

High-resolution x-ray luminescence computed tomography

Lun

2020

Medical Imaging 2020: Biomedical Applications in Molecular, Structural, and Functional Imaging

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High-resolution imaging modalities play a critical role for advancing biomedical sciences. Recently, x-ray luminescence computed tomography (XLCT) imaging was introduced as a hybrid molecular imaging modality that combines the high-spatial resolution of x-ray imaging and molecular sensitivity of optical imaging. The narrow x-ray beam based XLCT imaging has been demonstrated to achieve high spatial resolution, even at depth, with great molecular sensitivity. Using a focused x-ray beam as the excitation source, orders of magnitude of increased sensitivity has been verified compared with previous methods with a collimated x-ray beam. In this work, we demonstrate the high-spatial resolution capabilities of our focused x-ray beam based XLCT imaging system by scanning two sets of targets, differing in the target size, embedded inside of two tissue-mimicking cylindrical phantoms. Gd 2 O 2 S:Eu 3+ targets of 200 µm and 150 µm diameters were created and embedded with the same edge-to-edge distances as their diameters respectively. We scanned and reconstructed a single transverse section and successfully demonstrated that a focused x-ray beam with an average dual-cone size of 125 µm could separate the targets in both phantoms with good shape and location accuracy. We have also improved the current XLCT imaging system to make it feasible for fast three-dimensional XLCT scanning.

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“…The authors acknowledge that a preliminary version of this paper was previously published as a SPIE proceedings paper (Ref. 30 ).…”

Section: Acknowledgmentsmentioning

confidence: 99%

Focused x-ray luminescence imaging system for small animals based on a rotary gantry

et al. 2021

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. Significance: The ability to detect and localize specific molecules through tissue is important for elucidating the molecular basis of disease and treatment. Unfortunately, most current molecular imaging tools in tissue either lack high spatial resolution (e.g., diffuse optical fluorescence tomography or positron emission tomography) or lack molecular sensitivity (e.g., micro-computed tomography, ). X-ray luminescence imaging emerged about 10 years ago to address this issue by combining the molecular sensitivity of optical probes with the high spatial resolution of x-ray imaging through tissue. In particular, x-ray luminescence computed tomography (XLCT) has been demonstrated as a powerful technique for the high-resolution imaging of deeply embedded contrast agents in three dimensions (3D) for small-animal imaging. Aim: To facilitate the translation of XLCT for small-animal imaging, we have designed and built a small-animal dedicated focused x-ray luminescence tomography (FXLT) scanner with a scanner, synthesized bright and biocompatible nanophosphors as contrast agents, and have developed a deep-learning-based reconstruction algorithm. Approach: The proposed FXLT imaging system was designed using computer-aided design software and built according to specifications. nanophosphors doped with europium or terbium were synthesized with a silica shell for increased biocompatibility and functionalized with biotin. A deep-learning-based XLCT image reconstruction was also developed based on the residual neural network as a data synthesis method of projection views from few-view data to enhance the reconstructed image quality. Results: We have built the FXLT scanner for small-animal imaging based on a rotational gantry. With all major imaging components mounted, the motor controlling the gantry can be used to rotate the system with a high accuracy. The synthesized nanophosphors displayed distinct x-ray luminescence emission, which enables multi-color imaging, and has successfully been bound to streptavidin-coated substrates. Lastly, numerical simulations using the proposed deep-learning-based reconstruction algorithm has demonstrated a clear enhancement in the reconstructed image quality. Conclusions: The designed FXLT scanner, synthesized nanophosphors, and deep-learning-based reconstruction algorithm show great potential for the high-resolution molecular imaging of small animals.

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X-ray luminescence imaging for small animals

Cited by 5 publications

References 24 publications

X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb

X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb

High-resolution x-ray luminescence computed tomography

Focused x-ray luminescence imaging system for small animals based on a rotary gantry

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X-ray luminescence imaging for small animals

Cited by 5 publications

References 24 publications

X-ray excited luminescence spectroscopy and imaging with NaGdF4:Eu and Tb

X-ray excited luminescence spectroscopy and imaging with NaGdF4:Eu and Tb

High-resolution x-ray luminescence computed tomography

Focused x-ray luminescence imaging system for small animals based on a rotary gantry

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X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb

X-ray excited luminescence spectroscopy and imaging with NaGdF₄:Eu and Tb