X-ray fluorescence spectroscopy (XRF) applied to plant science: challenges towards <i>in vivo</i> analysis of plants

Montanha, Gabriel Sgarbiero; Santos, Eduardo; Marques, João Paulo Rodrigues; Almeida, Eduardo de; Reis, André Rodrigues dos; Carvalho, Hudson Wallace Pereira de

doi:10.1039/c9mt00237e

Cited by 34 publications

(24 citation statements)

References 55 publications

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“…2B-E). These findings are quite similar to those results reported in our previous studies (Jones et al 2019;Montanha et al 2020c). Furthermore, the 25 µm-thick Ti primary filter enabled us to record a remarkable increase of Mn signals due to the reduction of the background radiation reaching the X-ray detector.…”

Section: X-ray Induced Anatomical Histochemical and Ultrastructural C...supporting

confidence: 91%

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Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

Montanha

Marques

Santos

et al. 2022

Preprint

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X-ray fluorescence spectroscopy (XRF) is a powerful technique for the in vivo assessment of plant tissues. However, the potential X-ray exposure damages might affect the structure and elemental composition of living plant tissues leading to artefacts on the recorded data. Herein, we exposed soybean (Glycine max (L.) Merrill) leaves to several X-ray doses through a polychromatic benchtop microprobe X-ray fluorescence spectrometer, modulating the photon flux by adjusting either the beam size, focus, or exposure time. The structure and physiological responses of the irradiated plant tissues were investigated through light and transmission electron microscopy (TEM). Histochemical tests were also conducted. Depending on the dose, the X-ray exposure induced decreased K and X-ray scattering intensities, and increased Ca, P, and Mn signals on soybean leaves. Anatomical analysis indicated necrosis of the epidermal and mesophyll cells on the irradiated spots, where TEM images revealed the collapse of cytoplasm and cell-wall breaking. Furthermore, the histochemical analysis detected the production of reactive oxygen species, as well as inhibition of chlorophyll autofluorescence in these areas. Under certain X-ray exposure conditions, e.g., high photon flux and exposure time, XRF measurements may affect the soybean leaves structures and ultrastructure, elemental composition, cellular ultrastructure, and induce programmed cell death. These results shed light on the characterization of the radiation damage, and thus, help to assess the X-ray radiation limits and strategies for in vivo for XRF analysis.

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Section: X-ray Induced Anatomical Histochemical and Ultrastructural C...supporting

confidence: 91%

“…These findings are quite similar to those results reported in our previous studies (Jones et al . 2019; Montanha et al . 2020c).…”

Section: Discussionmentioning

confidence: 99%

Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

Montanha

Marques

Santos

et al. 2022

Preprint

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“…Microprobe X-ray fluorescence spectroscopy (μ-XRF) was employed to assess the elemental distribution among tomato fruits with or without BER symptoms. Due to its multielemental and nondestructive features, 30 μ-XRF afforded a simultaneous recording of Ca, P, K, Fe, and Zn intensities in the probed tissues.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Because XRF is a multielemental analytical technique, 30 the elemental distributions of P, K, Fe, and Zn were also recorded. However, no changes were observed in the distal part of tomato fruits that had been subjected to treatments (Figure S6).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Nickel Influences Urease Activity and Calcium Distribution in Tomato Fruits

Macedo

Montanha

Carvalho

et al. 2021

ACS Agric. Sci. Technol.

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In this study, we aimed to shed light on the effects of the Ni–Ca interaction on the activity of the urease enzyme and the incidence of blossom end rot (BER) in tomato (Solanum lycopersicum) fruits. Therefore, Micro-Tom tomato plants were grown under different levels of Ni (0.0 and 1.0 mg dm–3) and Ca2+ (0.35 and 0.70 g dm–3), both alone and in combination. Increased yields and urease activity, as well as inhibition of the incidence of BER, were observed for tomato fruits grown with Ni, even under low Ca availability (0.35 g dm–3). In addition, a microprobe X-ray fluorescence spectroscopy characterization of the distal part of the fruits revealed a sharp decrease in Ca content in the fruits that present BER symptoms.

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“…However, there is no standard method for this and more investigations are still needed. Some previous works have successfully employed the pXRF to assess the elemental composition of vegetation [37][38][39][40][41][42][43]. The concentrations of Cu, Zn, Pb, K, and Fe from different plant species growing in polluted mines were obtained via pXRF and were well correlated to laboratory-based methods [44].…”

Section: Introductionmentioning

confidence: 99%

Foliar Elemental Analysis of Brazilian Crops via Portable X-ray Fluorescence Spectrometry

Borges

Weindorf

Carvalho

et al. 2020

Sensors

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Foliar analysis is very important for the nutritional management of crops and as a supplemental parameter for soil fertilizer recommendation. The elemental composition of plants is traditionally obtained by laboratory-based methods after acid digestion of ground and sieved leaf samples. This analysis is time-consuming and generates toxic waste. By comparison, portable X-ray fluorescence (pXRF) spectrometry is a promising technology for rapid characterization of plants, eliminating such constraints. This worked aimed to assess the pXRF performance for elemental quantification of leaf samples from important Brazilian crops. For that, 614 samples from 28 plant species were collected across different regions of Brazil. Ground and sieved samples were analyzed after acid digestion (AD), followed by quantification via inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine the concentration of macronutrients (P, K, Ca, Mg, and S) and micronutrients (Fe, Zn, Mn, and Cu). The same plant nutrients were directly analyzed on ground leaf samples via pXRF. Four certified reference materials (CRMs) for plants were used for quality assurance control. Except for Mg, a very strong correlation was observed between pXRF and AD for all plant-nutrients and crops. The relationship between methods was nutrient- and crop-dependent. In particular, eucalyptus displayed optimal correlations for all elements, except for Mg. Opposite to eucalyptus, sugarcane showed the worst correlations for all the evaluated elements, except for S, which had a very strong correlation coefficient. Results demonstrate that for many crops, pXRF can reasonably quantify the concentration of macro- and micronutrients on ground and sieved leaf samples. Undoubtedly, this will contribute to enhance crop management strategies concomitant with increasing food quality and food security.

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X-ray fluorescence spectroscopy (XRF) applied to plant science: challenges towards in vivo analysis of plants

Abstract: X-ray fluorescence spectroscopy presents a unique capability to probe the elemental content of plants in vivo, besides combination with other non-destructive tools to assess physiological processes.

Cited by 34 publications

References 55 publications

Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

Nickel Influences Urease Activity and Calcium Distribution in Tomato Fruits

Foliar Elemental Analysis of Brazilian Crops via Portable X-ray Fluorescence Spectrometry

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