X‐chromosome inactivation analysis of isolated Reed‐Sternberg cells in nodular sclerosing Hodgkin's disease

Abstract

Summary. In spite of several studies of immunoglobulin (Ig) gene rearrangements in whole Hodgkin's disease (HD) tissues and in isolated single Reed-Sternberg (RS) cells, the issue of clonality of the RS cell in HD remains incompletely resolved. Analysis of X-chromosome inactivation patterns (XCIPs) can be used to determine whether cell populations are clonal in origin. By PCR ampli®cation of the human androgen receptor (HUMARA) loci using nested primers, we have studied XCIPs in six cases of HD of the nodular sclerosing (NS) subtype in which individual RS cells were isolated by micromanipulation from formalin-®xed, paraf®n-embedded tissue sections immunostained with CD30 or CD15. In order to assess whether a clonal population of RS cells might be present in NSHD tissues, we compared the XCIPs obtained from whole NSHD tissues with those obtained from single RS cells harvested from the same tissues. Whole tissues from all six cases of NSHD showed balanced HUMARA allelic patterns, whereas an average of 83% (range 77±91%) of single RS cells from each of the six tissues expressed the same high-or low-molecular weight allele, suggesting that a clonal population of RS cells was likely to be present in each case. These data are consistent with the presence of a clonal population of RS cells in NSHD tissues.

Identification of prognostic factors in classic Hodgkin lymphoma by integrating whole slide imaging and next generation sequencing

“…23 In cHL, however, X-chromosome inactivation analysis has been performed just to evidence clonality of HRSC. 56 Of note, the androgen receptor can be activated through androgen independent pathways 57 such as the PIK3CA pathway. We identified a loss of genetic material in the PIK3CA gene in a patient with an amplification in the AR gene.…”

Epstein-Barr-Virus-Infected CD15 (Lewis X)-Positive Hodgkin-Lymphoma-Like B Cells in Patients with Rheumatoid Arthritis

“…This study demonstrated that TKS-1, an EBV-positive LCL cell line that was spontaneously established from a patient with RA, expresses the HL markers CD15 and CD30 [26], and that PMA altered their expression to levels similar to those of H-RS cells. Hsu and Hsu examined the effect of PMA on the surface phenotype of H-RS cells obtained from the spleens of patients with HL and showed that CD15 expression persisted for at least three days, whereas that of CD30 gradually declined [24].…”