Wound healing property of milk in full thickness wound model of rabbit

Hemmati, Ali Asghar; Larki‐Harchegani, Amir; Shabib, Somayeh; Jalali, Amir; Rezaei, Anahita; Housmand, Gholamreza

doi:10.1016/j.ijsu.2018.04.030

Cited by 28 publications

(26 citation statements)

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“…Additionally, the allergy-protective effect of dietary acidic oligosaccharides is mediated by TGF-β in mice with cow milk allergy (Kerperien et al, 2018). Cow milk had significant beneficial effects on skin wound healing in a preclinical model (Hemmati et al, 2018). Even though oral mucosal healing was considered the ideal system of wound resolution (Iglesias-Bartolome et al, 2018), oral mucositis is a frequent and severe complication of chemotherapy in children (Cheng et al, 2008) and adults with cancer (Lalla et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula

Panahipour

Tabatabaei

Gruber

2020

Journal of Dairy Science

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Hypoallergenic formulas are recommended for infants who are not breastfed and cannot tolerate cow milk formulas due to allergy. These formulas are hydrolyzed to break down larger protein chains into shorter, easy-to-digest, and potentially less allergenic proteins. Hydrolysis, however, possibly occurs at the expense of the transforming growth factor beta (TGF-β) and anti-inflammatory activity that is inherent in regular formula. Our objective was to determine the TGF-β and the anti-inflammatory activity of commercially available hypoallergenic and regular formulas. Human gingival fibroblasts were incubated with reconstituted formulas followed by detection of TGF-β target genes and activation of Smad2/3 signaling. Gingival fibroblasts and the oral squamous cell carcinoma cell line HSC-2 were also exposed to formulas before adding interleukin (IL)1β and tumor necrosis factor (TNF) α to provoke expression of pro-inflammatory cytokines. For murine bone marrow-derived macrophages, pro-inflammatory cytokine expression was stimulated with saliva. Changes in p65 nuclear translocation and phosphorylation of smad3 and p38 were analyzed by immunostaining. Our study demonstrated that regular formula, but not hypoallergenic formula, enhanced the expression of TGF-β target genes IL11, PRG4, and NOX4 in gingival fibroblasts. Hypoallergenic formulas also failed to initiate nuclear translocation of Smad2/3 and phosphorylation of Smad3. Moreover, regular formulas were more potent than hypoallergenic formulas in reducing the expression of pro-inflammatory cytokines in gingival fibroblasts, HSC-2 epithelial cells, and murine bone marrow macrophages. Hypoallergenic and regular formulas had a similar capacity to reduce p65 nuclear translocation and phosphorylation of p38 in fibroblasts. These findings suggest that hypoallergenic formulas lack in vitro TGF-β activity and have a lower anti-inflammatory activity compared with regular formulas.

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Section: Introductionmentioning

confidence: 99%

Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula

Panahipour

Tabatabaei

Gruber

2020

Journal of Dairy Science

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“…Farklı memeli türlerinden elde edilen sütlerin in vitro ortamda çeşitli kanser hatları üzerinde denendiği çalışmalar literatürde mevcuttur [19,[23][24][25][26]. Bazı çalışmalarda memeli sütleri veya içerikleri antikanser aktivite gösterirken [19,23,24,27], bazı çalışmalarda ise bizim sonuçlarımıza benzer şekilde hücre proliferasyonu gözlenmiştir [25]. Kısrak, eşek, inek, deve sütlerinin ve bu sütlerden izole edilen kazein ve kesik süt suyu proteinlerinin insan meme kanseri (MCF-7) hücre hattı üzerinde sitotoksik etki gösterdikleri sonucuna ulaşılmıştır [19].…”

Section: çIzik Testi Bulgularıunclassified

“…Yapılan bir çalışmada, düşük yağ içerikli inek süt proteinlerinin ve kesik süt suyu proteinlerinin tavşanlarda yara kapanma hızını arttırdığı sonucuna ulaşılmıştır. İnek sütünün ve bileşenlerinin hücre onarım sürecini hızlandırdığı ve yara iyileştirme üzerinde olumlu etkisi olduğu görülmüştür [27]. Başka bir çalışmada, deve sütünden elde edilen kesik süt suyu proteininin diyabetik farelerde yaranın daha çabuk kapanmasına sebep olduğu belirtilmiştir [34].…”

Section: çIzik Testi Bulgularıunclassified

Eşek sütü ve bazı bileşenlerinin in vitro ortamda antikanser/yara iyileştirme aktivitesinin incelenmesi

Eroğlu

Öksüz

2020

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Bu çalışmada, eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda antikanser ve yara iyileştirme potansiyelinin araştırılması amaçlanmıştır. Öncelikle eşek sütünün pH değeri değiştirilerek kazein ve kesik süt suyu proteinleri elde edilmiştir. Eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda insan prostat kanseri hücre hattı (PC-3) üzerindeki antikanser aktivitesi MTT hücre proliferasyon testi kullanılarak incelenmiş olup antikanser aktivite bulunamamıştır. MTT denemelerinde özellikle kazein proteininin %140 hücre proliferasyonuna sebep olduğu gözlenmiştir. Bu doğrultuda, insan fibroblast hücre hattı (BJ) üzerinde çizik testi uygulanarak bu proteinlerin hücre migrasyonu ve yara iyileştirici etkileri incelenmiştir.Eşek sütü, kazein ve kesik süt suyu proteini (3200 µg/ml) kontrol grubuna kıyasla hücre migrasyonunda ve in vitro yara kapanma hızında anlamlı artışa sebep olmuştur. Bu çalışma ile eşek sütünün ve içerisindeki diğer proteinlerin in vitro ortamda antikanser aktivite göstermeyip hücre proliferasyonuna sebep olduğu ve yara iyileştirme amaçlı kullanılabileceği gösterilmiştir.

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“…The contraction of myofibroblasts approximates the wound edges, reducing the wound area. Many kinds of cytokines and growth factors play an essential role in skin wound repair [5].…”

Section: Introductionmentioning

confidence: 99%

The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

et al. 2021

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Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.

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Wound healing property of milk in full thickness wound model of rabbit

Abstract: It can, therefore, be concluded that low-fat cow's milk has significant beneficial effects on skin wound healing. Therefore, it may be used as a healing agent in different types of the wound in humans after certain clinical trials.

Cited by 28 publications

References 28 publications

Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula

Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula

Eşek sütü ve bazı bileşenlerinin in vitro ortamda antikanser/yara iyileştirme aktivitesinin incelenmesi

The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

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