Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

Abstract

The effects of different extracts obtained from Jasione montana L. (JM1–JM6) and their main metabolites on biological processes during wound healing were evaluated. The effect on wound closure in the scratch test was established, and collagen type I synthesis and anti-inflammatory effects were assessed by flow cytometry in a human dermal fibroblast model (PCS-201-012). Additionally, the antioxidant activity (DPPH and FRAP) and degree of inhibition of elastase participating in the proliferation processes of skin fibroblasts were determined in an in vitro model. The extracts and fractions were analyzed using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) to quantitatively characterize their main polyphenolic compounds. The high antioxidant activity of the JM4–JM5 fractions correlated with the content of luteolin and its derivative 7-O-glucoside. Luteolin also showed the highest anti-elastase activity with an IC50 value of 39.93 ± 1.06 μg/mL, and its substantial content in the JM4 fraction presumably determines its activity (359.03 ± 1.65 μg/mL). At lower concentrations (<50 μg/mL) of all extracts, cell proliferation and migration were significantly stimulated after 24 h of treatment. The stimulation of cell migration was comparable with that of allantoin, which was used as a positive control. However, most of the tested extracts showed limited capacity to affect collagen type I biosynthesis. Moreover, the tested samples exhibited a complex effect on cytokine secretion, and the strongest anti-inflammatory activity through the moderation of IL-1β, IL-6 and IL-8 was observed for JM4 and luteolin. Based on the obtained results of the quantitative analysis, the anti-inflammatory activity of JM4 may be due to the high content of luteolin. In summary, extracts from J. montana, which is flavonoid-rich, promote the viability and accelerate the migration of fibroblasts as well as moderate oxidant and inflammatory processes and elastase activity. Hence, they may be potentially useful for topical therapeutic applications to stimulate the wound healing process.

Glycerol and Natural Deep Eutectic Solvents Extraction for Preparation of Luteolin-Rich Jasione montana Extracts with Cosmeceutical Activity

“…The eutectic-based extract (PG-50-TP) demonstrated a statistically significant superior inhibitory effect with an IC 50 value of 4.14 ± 0.02 µL of extract/mL. This confirms the results of a previous study which found that luteolin-rich J. montana extracts are excellent inhibitors of elastase [ 18 ].…”

“…High antiradical activity of OPT-TP is not surprising because phenolics, due to their chemical structures, may donate hydrogen to free radicals and thus strongly contribute to the antioxidant activity of the prepared extracts [ 41 ]. A previous study has shown that luteolin-rich J. montana extracts are potent radical scavengers [ 18 ]; however, it is difficult to compare the results of that study to those obtained in this work due to different measurement units. In the same work, performed on a smaller number of extracts, it was reported that the RSA correlates with luteolin content [ 18 ]; however, the results obtained in this study indicate that other phenolics present in the extracts also play an important part in the RSA of the extracts.…”

“…For example, it was found that J. montana extracts may promote the viability and accelerate the migration of fibroblasts. Furthermore, luteolin-rich J. montana extracts were found to be able to modulate oxidant and inflammatory processes, as well as impede elastase activity [ 18 ]. Anti-melanoma activity of luteolin-rich extracts of J. montana was also confirmed [ 16 ].…”

Formulation and Characterization of Amomum Compactum Sol. Ex Maton Nano Emulsion and Its Antibacterial and Wound Healing Activity

“…These chemicals increase fibroblast viability, migration, and collagen formation, speeding wound healing. These chemicals' antioxidant characteristics limit ROS and oxidative stress, which aid wound healing (Barku, 2019;Juszczak et al, 2022). The study found that ethanol cardamom extracts better suppress harmful microorganisms like E. coli and S. aureus than methanol extracts.…”

Efficacy of methanolic extracts of some medicinal plants on wound healing in diabetic rats

“…Migration assay At a density of 35,000 cells per well, fibroblasts were seeded and incubated until confluent. Using a 200 μl pipette tip as described byJuszczak et al[39], a scratch was made in each well. Before adding the medium conditioned with different concentrations of each plant extract (10 and 20 μg/ml), the media was withdrawn and the cells were rinsed * Significant at P < 0.05 over the negative control.…”