Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts

Abstract

Background/Aim:Wounds are the outcome of injuries to the skin that interrupt the soft tissue. Healing of a wound is a complex and long-drawn-out process of tissue repair and remodeling in response to injury. A large number of plants are used by folklore traditions for the treatment of cuts, wounds and burns. Moringa oleifera (MO) is an herb used as a traditional folk medicine for the treatment of various skin wounds and associated diseases. The underlying mechanisms of wound healing activity of ethyl acetate fraction of MO leaves extract are completely unknown.Materials and Methods:In the current study, ethyl acetate fraction of MO leaves was investigated for its efficacy on cell viability, proliferation and migration (wound closure rate) in human normal dermal fibroblast cells.Results:Results revealed that lower concentration (12.5 µg/ml, 25 µg/ml, and 50 µg/ml) of ethyl acetate fraction of MO leaves showed remarkable proliferative and migratory effect on normal human dermal fibroblasts.Conclusion:This study suggested that ethyl acetate fraction of MO leaves might be a potential therapeutic agent for skin wound healing by promoting fibroblast proliferation and migration through increasing the wound closure rate corroborating its traditional use.

In Vitro Wound Healing Potential of Flavonoid C-Glycosides from Oil Palm (Elaeis guineensis Jacq.) Leaves on 3T3 Fibroblast Cells

“…However, the cells were more viable at low concentrations than higher concentrations as the number of cells decreased as the concentration increased. The results are in agreement with previous reports that mentioned the Spirulina platensis crude extracts were also non-toxic at low concentrations [19], as well as in Moringa oleifera crude extracts and fractions [7,23].…”

“…In this study, the selection of water-soluble tetrazolium salts, WST-8 assay for the cell proliferation study in comparison with MTT assay is mainly due to the high sensitivity of WST-8 assay to express the numbers of living cells resulted from the amount of the yellow-colour formazan dye, generated by the activities of dehydrogenases in cells since the dehydrogenase activities in cells are directly proportional to the number of living cells. Several studies are in agreement with the high sensitivity shown by WST-8 assay in cell proliferation activity [7,26,27].…”

“…The cell proliferation assay was performed as described previously by Gothai et al [7], with slight modifications. 3T3 cells were seeded on 96 well plates at a density of 1 × 10 5 (in 100 µL medium) per well and incubated at 37 • C for 24 h. The medium was replaced with 100 µl sample solutions with different concentrations (1.56, 3.13, 6.25 and 12.5 µg/mL) for 48 h. 10 µL of cell counting kit-8 (CCK-8) was then added into each of the wells and incubated for another 4 h according to the manufacturer's instructions (Dojindo Lab, Kumamoto, Japan).…”

“…The migration and proliferation of cells was examined using the scratch assay method Gothai et al [7], and Nur et al [19], with slight modifications. 3T3 cells were seeded in 24-well plates at a density of 3 × 10 5 cells/well and allowed to grow for 24 h at 37 • C and 5 % CO 2 .…”

HPLC methods for quality control of Moringa oleifera extract using isothiocyanates and astragalin as bioactive markers

“…(Moringaceae family) is a plant commonly found in several tropical and subtropical countries. Traditionally, M. oleifera has been used for its medicinal benefits, including antiinflammation [1][2][3] , anti-oxidation [4][5][6] , anti-bacterial, and anti-fungal [7][8][9] properties, wound healing [10][11][12] , and skin revitalization 13 . Currently, many cosmetics and natural health products use M. oleifera extracts as an active ingredient.…”