Wound healing evaluation of self-nanoemulsifying drug delivery system containing Piper cubeba essential oil

Abstract

The objective of this work was to characterize and evaluate wound healing potential of Piper cubeba oil (PO) via selfnanoemulsifying drug delivery system (SNEDDS) in comparison with standard gentamycin. PO loaded SNEDDS was prepared by low energy emulsification technique and characterized for thermodynamic stability, self-emulsification power and various physico-chemical parameters. An optimal formula of PO SNEDDS was subjected to wound healing evaluation, collagen determination and histomorphological examination in female Wistar rats as compared with pure PO and standard antibiotic/gentamycin. An optimal formula of PO SNEDDS showed significant wound healing effects in Wistar female rats in comparison with pure PO. However, wound healing effects of optimized SNEDDS were comparable with standard gentamycin. An optimized formulation also indicated significant enhancement in collagen content (0.82 mg/g) in comparison with pure PO (0.53 mg/g) and negative control (0.33 mg/g). While, the collagen content of SNEDDS (0.82 mg/g) treated rats were comparable with standard gentamycin treated animals (0.98 mg/g). Histopathological examinations of optimized SNEDDS treated animals showed no signs of inflammatory cells which indicated that prepared SNEDDS was safe and nontoxic to rats. The results obtained in this work showed the potential application of SNEDDS in enhancement of the wound healing activity of PO upon oral administration.

Preparation of a novel curcumin nanoemulsion by ultrasonication and its comparative effects in wound healing and the treatment of inflammation

“…Excision of the wound was performed, with an approximate predetermined area of 500 mm 2 maximum thickness on the anterior-dorsal side of each female rat. 33,38 All the female rats topically received the test formulations once daily for their respective group, for 24 days. The percentage of wound contractions and their healing time were the best parameters to analyze the wound healing.…”

“…The percentage area of wound contractions were calculated using a graph sheet. 33,38 Total wound healing area and closing area were determined.…”

Polymeric Carriers Designed for Encapsulation of Essential Oils with Biological Activity

“…Self-nanoemulsifying drug delivery system (SNEDDS) formulations in water, based on Piper cubeba EO and synthetic and nontoxic components, namely Sefsol-218 (a propylene glycol caprylate), Triton-X100 (a nonionic surfactant that has a hydrophilic polyethylene oxide chain and an aromatic hydrocarbon lipophilic or hydrophobic group), Transcutol-HP (a hydrophilic co-surfactant), were prepared [ 159 , 160 ]. The prepared systems were investigated for wound healing potential in rats in comparison with pure Piper cubeba oil and standard gentamycin.…”

Antifungal activity of Piper cubeba L. extract on spoilage fungi isolated from tomato, grapes and lemon

“…The pepper of the perennial shrub was originated from Indonesia and mostly cultivated in Java and Sumatra, and some part of India (Nahak and Sahu, 2011). Besides used as flavoring ingredients in food, the pepper have been widely reported in traditional medicines practices mainly to cure abdominal pain, asthma, intestinal disease, dysentery, bronchitis, cough, tuberculosis and rheumatism (Bos et al, 2007;Shakeel et al, 2019). The pepper is rich with the valuable phytochemical compounds such as alkaloids (piperine and cubebin), amides, flavanones, flavones, lignans, neolignans, terpenes, sesquiterpenes and propenylphenols (Parmar et al, 1997).…”