Wound healing effects of nanoemulsion containing clove essential oil

Abstract

The aim of this study was to investigate the wound healing effects of clove oil (CO) via its encapsulation into nanoemulsion. Optimized nanoemulsion (droplet size of 29.10 nm) was selected for wound healing investigation, collagen determination, and histopathological examination in rats. Optimized nanoemulsion presented significant would healing effects in rats as compared to pure CO. Nanoemulsion also presented significant enhancement in leucine content (0.61 mg/g) as compared to pure CO (0.50 mg/g) and negative control (0.31 mg/g). Histopathology of nanoemulsion treated rats showed no signs of inflammatory cells. These results suggested that nanoemulsion of CO was safe and nontoxic.

Enhanced acyclovir delivery using w/o type microemulsion: preclinical assessment of antiviral activity using murine model of zosteriform cutaneous HSV-1 infection

“…The transmission electron micrograph revealed spherical nature of the microemulsion droplets (Figure 2(A)). The mean diameter of the optimized formulation was found to be 198.2 nm with polydispersity index of 0.180 (Figure 2(B)) [34,35]. The zeta potential of the prepared microemulsion was found to be 0.279 mV.…”

Wound Healing Study of Eucalyptus Essential Oil Containing Nanoemulsion in Rat Model

“…The severity of the inflammation in the healed areas was investigated by counting the inflammatory cell infiltration per field in each group. The presence of epithelization, inflammatory cell infiltra-tion, fibroblast proliferation, neovascularization and collagen deposition on healed area of skin were measured using a modified 0-5 numerical scale stated below 12 : Score 0: Absence Score 1: Occasional presence Score 2: Light scattering Score 3: Abundance Score 4: Confluence of cells Score 5: Fibres…”

“…However, the wound healing activities of most of the essential oils using nanosized drug delivery systems have rarely been studied in literature. The wound healing activity of clove essential oil CEO via nanoemulsion has been reported recently in literature 12 . Alam et al got significant wound healing effects of CEO loaded nanoemulsion as compared with pure CEO 12 .…”

“…The wound healing activity of clove essential oil CEO via nanoemulsion has been reported recently in literature 12 . Alam et al got significant wound healing effects of CEO loaded nanoemulsion as compared with pure CEO 12 . The wound healing effects of EEO topical nanoemulsion have also been reported in liter-Abstract: The objective of this investigation was to develop nanoemulsion formulations of Eucalyptus essential oil (EEO) and to evaluate its wound healing effects in comparison with standard gentamycin in rat model.…”

“…Hence, the objective of this research work was to develop suitable nanoemulsion formulations of EEO and to investigate its wound healing effects in rat model as compared with pure EEO and standard antibiotic gentamycin after oral administration. Macroemulsions were not prepared in this work because nanoemulsions have lower droplet size in comparison with macroemulsions and these systems are more suitable for enhancing absorption and therapeutic activity of essential oils 11,12,27 . Various nanoemulsion formulations of EEO were prepared using aqueous phase titration method via construction of pseudoternary phase diagrams.…”