Wound healing and arm regeneration in Ophioderma longicaudum and Amphiura filiformis (Ophiuroidea, Echinodermata): comparative morphogenesis and histogenesis

Biressi, Anna; Zou, Ting; Dupont, Samuel; Dahlberg, Carl; Benedetto, Cristiano Di; Bonasoro, F.; Thorndyke, Michael C.; Carnevali, M. Daniela Candia

doi:10.1007/s00435-009-0095-7

Cited by 55 publications

(107 citation statements)

References 34 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on histological studies, Wood et al (Wood et al, 2008) suggested that unfed A. filiformis increases protein metabolism by the breakdown of muscle tissues to fuel increased energetic demands in response to seawater acidification. It is likely that enhanced catabolism and cell turnover/dedifferentiation of muscle tissues following traumatic amputation in brittlestars and crinoids is related to cell differentiation to produce stem cells to regenerate lost tissues rather that fueling calcification processes (Candia Carnevali and Bonasoro, 2001;Biressi et al, 2010). However, despite increased NH 4 + excretion rates, the present study could not demonstrate any changes in ratios between ADM and DM between pH treatments, which would have indicated an increased utilization of muscle tissue or other amino acid compounds as an energy source.…”

Section: Research Articlecontrasting

confidence: 69%

Energy metabolism and regeneration impaired by seawater acidification in the infaunal brittlestar,Amphiura filiformis

Casties

Stumpp

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Seawater acidification due to anthropogenic release of CO 2 as well as the potential leakage of pure CO 2 from sub-seabed carbon capture storage (CCS) sites may impose a serious threat to marine organisms. Although infaunal organisms can be expected to be particularly impacted by decreases in seawater pH, as a result of naturally acidified conditions in benthic habitats, information regarding physiological and behavioral responses is still scarce. Determination of P O2 and P CO2 gradients within burrows of the brittlestar Amphiura filiformis during environmental hypercapnia demonstrated that besides hypoxic conditions, increases of environmental P CO2 are additive to the already high P CO2 (up to 0.08 kPa) within the burrows. In response to up to 4 weeks exposure to pH 7.3 (0.3 kPa P CO2 ) and pH 7.0 (0.6 kPa P CO2 ), metabolic rates of A. filiformis were significantly reduced in pH 7.0 treatments, accompanied by increased ammonium excretion rates. Gene expression analyses demonstrated significant reductions of acid-base (NBCe and AQP9) and metabolic (G6PDH, LDH) genes. Determination of extracellular acid-base status indicated an uncompensated acidosis in CO 2 -treated animals, which could explain the depressed metabolic rates. Metabolic depression is associated with a retraction of filter feeding arms into sediment burrows. Regeneration of lost arm tissues following traumatic amputation is associated with significant increases in metabolic rate, and hypercapnic conditions (pH 7.0, 0.6 kPa) dramatically reduce the metabolic scope for regeneration, reflected in an 80% reduction in regeneration rate. Thus, the present work demonstrates that elevated seawater P CO2 significantly affects the environment and the physiology of infaunal organisms like A. filiformis.

show abstract

Section: Research Articlecontrasting

confidence: 69%

Energy metabolism and regeneration impaired by seawater acidification in the infaunal brittlestar,Amphiura filiformis

Casties

Stumpp

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Immune cells with phagocytic potential have been identified across the invertebrates, e.g. within Arthropoda [81] and Echinodermata [89]. During wound repair, after clot formation, specialized immune cells infiltrate the wound site and phagocytose cell debris and foreign organisms [90].…”

Section: (Iii) Clot Formationmentioning

confidence: 99%

“…During wound repair, after clot formation, specialized immune cells infiltrate the wound site and phagocytose cell debris and foreign organisms [90]. Infiltrated cells then proliferate, forming granulation tissue, which consists of multiple cell types, collagen and a basic ECM, and provides a platform for re-epithelialization [81,89]. Although there are differences among species, these cellular phases of wound healing have been documented within numerous invertebrates [81,91].…”

Section: (Iii) Clot Formationmentioning

confidence: 99%

Towards an integrated network of coral immune mechanisms

2012

View full text Add to dashboard Cite

Reef-building corals form bio-diverse marine ecosystems of high societal and economic value, but are in significant decline globally due, in part, to rapid climatic changes. As immunity is a predictor of coral disease and thermal stress susceptibility, a comprehensive understanding of this new field will likely provide a mechanistic explanation for ecological-scale trends in reef declines. Recently, several strides within coral immunology document defence mechanisms that are consistent with those of both invertebrates and vertebrates, and which span the recognition, signalling and effector response phases of innate immunity. However, many of these studies remain discrete and unincorporated into the wider fields of invertebrate immunology or coral biology. To encourage the rapid development of coral immunology, we comprehensively synthesize the current understanding of the field in the context of general invertebrate immunology, and highlight fundamental gaps in our knowledge. We propose a framework for future research that we hope will stimulate directional studies in this emerging field and lead to the elucidation of an integrated network of coral immune mechanisms. Once established, we are optimistic that coral immunology can be effectively applied to pertinent ecological questions, improve current prediction tools and aid conservation efforts.

show abstract

“…Histological studies have divided arm regeneration in brittle stars into 4 main phases: wound repair with re-epithelialisation; early regeneration with complete healing and cell proliferation at the site of damage; intermediate regeneration resulting in blastema formation; and, finally, advanced regeneration with the development of a miniature arm (Biressi et al 2010). Based on gross morphological observations, it is production of a regenerative bud and arm elongation that constitutes the delayed phase in Ophionotus victoriae (Clark et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, regeneration in the temperate brittle star Ophioderma longicaudum has been described to have an even slower regeneration rate of 0.17 mm wk −1 ; although, it took only 3 wk to initiate regeneration and generate a blastema and measureable regenerative bud (Biressi et al 2010). Here we describe further regeneration studies on another common Antarctic brittle star, Ophiura crassa Mortensen, 1936, to further elucidate regeneration processes in ophiuroids and in particular to understand the effect of temperature.…”

Section: Introductionmentioning

confidence: 99%

Slow arm regeneration in the Antarctic brittle star Ophiura crassa (Echinodermata, Ophiuroidea)

Clark¹,

Souster²

2012

Aquat. Biol.

View full text Add to dashboard Cite

Regeneration of arms in brittle stars is thought to proceed slowly in low temperature environments. Here a survey of natural arm damage and arm regeneration rates is documented in the Antarctic brittle star Ophiura crassa. This relatively small ophiuroid, a detritivore found amongst red macroalgae, displays high levels of natural arm damage and repair. This is largely thought to be due to ice damage in the shallow waters it inhabits. The time scale of arm regeneration was measured in an aquarium-based 10 mo experiment. There was a delayed regeneration phase of 7 mo before arm growth was detectable in this species. This is 2 mo longer than the longest time previously described, which was in another Antarctic ophiuroid, Ophionotus victoriae. The subsequent regeneration of arms in O. crassa occurred at a rate of approximately 0.16 mm mo −1 . To date, this is the slowest regeneration rate known of any ophiuroid. The confirmation that such a long delay before arm regeneration occurs in a second Antarctic species provides strong evidence that this phenomenon is yet another characteristic feature of Southern Ocean species, along with deferred maturity, slowed growth and development rates. It is unclear whether delayed initial regeneration phases are adaptations to, or limitations of, low temperature environments.KEY WORDS: Ophiura crassa · Ophionotus victoriae · Autoecology · Ophioderma longicaudum · Brooder · Q 10 coefficientResale or republication not permitted without written consent of the publisher

show abstract

Wound healing and arm regeneration in Ophioderma longicaudum and Amphiura filiformis (Ophiuroidea, Echinodermata): comparative morphogenesis and histogenesis

Cited by 55 publications

References 34 publications

Energy metabolism and regeneration impaired by seawater acidification in the infaunal brittlestar,Amphiura filiformis

Energy metabolism and regeneration impaired by seawater acidification in the infaunal brittlestar,Amphiura filiformis

Towards an integrated network of coral immune mechanisms

Slow arm regeneration in the Antarctic brittle star Ophiura crassa (Echinodermata, Ophiuroidea)

Contact Info

Product

Resources

About