Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals

Abstract

<jats:title>Abstract</jats:title><jats:sec id="j_jcim-2019-0133_s_010_w2aab3b7d111b1b6b1aab1c14b1Aa"><jats:title>Background</jats:title><jats:p>Diabetic wound are the major problem of society because of its delay healing of wound in hyperglycemia patient. The target of this study need to know the gallic acid compelling synergistically wound healing in diabetic animals as it is available in fruit extract or is more effective in given pure form in diabetic animals.</jats:p></jats:sec><jats:sec id="j_jcim-2019-0133_s_011_w2aab3b7d111b1b6b1aab1c14b2Aa"><jats:title>Methods</jats:title><jats:p>The wound healing effects of fruit extract <jats:italic>Terminalia bellerica</jats:italic> Roxb. and gallic acid were evaluated in experimentally induced hyperglycemic animals. In this investigation, excision and dead space wound models were used. The contraction wound %, re-epithelialization days, area of scar, histopathological examination of tissue, hydroxyproline level and weight of tissue granuloma were evaluated in this study.</jats:p></jats:sec><jats:sec id="j_jcim-2019-0133_s_012_w2aab3b7d111b1b6b1aab1c14b3Aa"><jats:title>Results</jats:title><jats:p><jats:italic>Terminalia bellerica</jats:italic> fruit extract (400 mg/Kg) and gallic acid (200 mg/Kg) have been ingested by orally in streptozotocin induced diabetic rats. They were founded significantly (p&lt;0.05) improved percentage wound contraction and decreased the size of scar area and days of re-epithelialization in excision wound model. Also, they were improved the level of hydroxyproline level in diabetic rats as compared to diabetic control group in dead space wound model. Standard drug vitamin C was also founded significantly improved wound healing activity in rats. These results suggested that treated groups accelerated wound healing activity in diabetic rat.</jats:p></jats:sec><jats:sec id="j_jcim-2019-0133_s_013_w2aab3b7d111b1b6b1aab1c14b4Aa"><jats:title>Conclusions</jats:title><jats:p>The observations and results obtained in the present study indicated the wound healing efficacy of fruit ethanolic extract and gallic acid in diabetic animals. Yet, the wound healing effect was increasingly reported with gallic acid.</jats:p></jats:sec>

The Role of Antioxidants on Wound Healing: A Review of the Current Evidence

“…Rocasalbas [62] 2013 Gelatin hydrogel X Díaz-González [63] 2012 X Bektas N [64] 2020 Hydrogel X X Chitosan + gallic acid Thi [31] 2020 Gelatin hydrogel X X Singh [65] 2019 X Yang [66] 2016 X Stefanov [30] 2016 X X (ExV)…”

“…The group treated with gallic acid effectively showed improved re-epithelialization and wound remodeling compared to the other groups. Researchers believe that this injectable ROS-scavenging hydrogel has great potential for wound treatment and tissue regeneration, where oxidative damage by ROS contributes to the pathogenesis In another study, Singh et al [65] investigated the wound healing activity of the Terminalia bellerica fruit ethanolic extract compared with its active constituent, gallic acid, in experimentally induced diabetic animals. Rats were divided into seven groups: group I normal control (saline 5 mL/Kg), group II normal experimental (Terminalia bellerica extract 400 mg/Kg), group III normal experimental (gallic acid 200 mg/Kg), group IV diabetic control (vehicle only 5 mL/Kg), group V diabetic experimental (Terminalia bellerica extract 400 mg/Kg), group VI diabetic experimental (gallic acid 200 mg/Kg), group VII diabetic standard (Vitamin C 200 mg/Kg).…”

The potential application of natural products in cutaneous wound healing: A review of preclinical evidence

“…GA has antioxidant, antiinflammatory, and analgesic effects (Comino-Sanz et al, 2021). Studies have shown that GA can improve wound healing in diabetic rats, and promote neovascularization, and collagen deposition during wound healing in diabetic animals (Singh et al, 2019). In vitro studies have shown that GA upregulates the expression of antioxidant genes, stimulates cell migration on fibroblasts, and activates healing factors (Yang et al, 2016).…”

“…In general, Ginsenosides Rb1 (Zhang et al, 2021), Lupeol (Pereira Beserra et al, 2020), garlic acid (Singh et al, 2019), Ferulic acid (Dwivedi et al, 2022), Hesperdin (Jagetia and Rao, 2022), Aloin (Liu et al, 2015), Simmondsia cheinensis (Ranzato et al, 2011), Snail mucins (Gugliandolo et al, 2021), Angelica Sinensis ethanol extract (Bai et al, 2012b), Apigenin (Choi et al, 2016)have important potential to promote wound healing, and the molecular mechanism should be further studied. This conclusion is supported by desirable results, both in animal and cell experiments.…”

The Role of Antioxidants on Wound Healing: A Review of the Current Evidence

“…The group treated with gallic acid effectively showed improved re-epithelialization and wound remodeling compared to the other groups. Researchers believe that this injectable ROS-scavenging hydrogel has great potential for wound treatment and tissue regeneration, where oxidative damage by ROS contributes to the pathogenesis In another study, Singh et al [61] investigated the wound healing activity of the Terminalia bellerica fruit ethanolic extract compared with its active constituent, gallic acid, in experimentally induced diabetic animals. Rats were divided into seven groups: group I normal control (saline 5 mL/Kg), group II normal experimental (Terminalia bellerica extract 400 mg/Kg), group III normal experimental (gallic acid 200 mg/Kg), group IV diabetic control (vehicle only 5 mL/Kg), group V diabetic experimental (Terminalia bellerica extract 400 mg/Kg), group VI diabetic experimental (gallic acid 200 mg/Kg), group VII diabetic standard (Vitamin C 200 mg/Kg).…”