Wound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model

Zhao, Shichang; Li, Le; Wang, Hui; Zhang, Yadong; Cheng, Xu; Zhou, Nina; Rahaman, Mohamed N.; Liu, Zhongtang; Huang, Wenhai; Zhang, Changqing

doi:10.1016/j.biomaterials.2015.02.112

Cited by 332 publications

(272 citation statements)

References 54 publications

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“…29 VEGF plays a prominent role in regulation of physiologic angiogenesis and it is a potent mitogen for vascular endothelial cells. 30,31 Therefore, we focused on whether the Col/BG nanofibers can induce the secretion of VEGF in HDFs.…”

Section: Biological Effects Of Hdfs Induced By the Col/bg Nanofibersmentioning

confidence: 99%

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Zhou¹,

Sui²,

Mo³

et al. 2017

IJN

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The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus . It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing.

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Section: Biological Effects Of Hdfs Induced By the Col/bg Nanofibersmentioning

confidence: 99%

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Zhou¹,

Sui²,

Mo³

et al. 2017

IJN

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show abstract

“…Glass fibers, in addition to their unique mechanical and optical properties for optic applications, are also promising for biomedical applications [5]. They have the potential to mimic the natural morphology of collagenous extracellular matrix, which may favour cell proliferation and differentiation [6,7]. These features make glass fibers interesting building blocks for developing scaffolds and composites for bone regeneration applications [8,9].…”

Section: Introductionmentioning

confidence: 97%

Preparation and characterization of fibrous chitosan-glued phosphate glass fiber scaffolds for bone regeneration

Zheng

Wei

et al. 2015

J Mater Sci: Mater Med

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Phosphate glass fibers (PGF) have emerged as promising building blocks for constructing bone scaffolds. In this study, fibrous scaffolds (PGFS) were fabricated using a facile binding method at room temperature. PGFS exhibited an extracellular matrix-like morphology and were composed of PGF as matrix and chitosan as the natural binding glue. They showed an interconnected porous structure with a porosity of ~87% and pore size of 100-500 µm. PGFS exhibited the typical compressive stress-strain behaviour of highly porous, low-density, open-cell scaffolds. Their yield stress and modulus were ~0.38 and ~2.84 MPa, respectively, with the strength being higher than the lower bound of the compressive strength of cancellous bone. PGFS were degradable and the weight loss was about 25% after immersion in stimulated body fluid (SBF) for 28 days. In addition, the yield stress and the modulus decreased with increasing immersion time in SBF. Apatite formation could be detected on the surface of PGFS within 7 days of immersion in SBF. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that PGFS were non-cytotoxic against bone marrow stromal cells (bMSCs) after culture for up to 72 h. These results suggest that PGFS could be promising scaffolds for bone regeneration applications.

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“…Therefore, incorporation of the cells into an appropriate polymeric or synthetic scaffold has shown promising results in the augmentation of cell engraftment [22]. Up to now, various biomaterials and multiple growth factors have been used to regenerate skin lesions, particularly in chronic wounds [23,24]. Considerable attention has been paid to biologic scaffolds derived from the extracellular matrix (ECM) of native tissues due to the safety and efficacy of such natural scaffolds [25,26].…”

Section: Introductionmentioning

confidence: 99%

Accelerated wound healing in a diabetic rat model using decellularized dermal matrix and human umbilical cord perivascular cells

et al. 2016

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There is an unmet clinical need for novel wound healing strategies to treat full thickness skin defects, especially in diabetic patients. We hypothesized that a scaffold could perform dual roles of a biomechanical support and a favorable biochemical environment for stem cells. Human umbilical cord perivascular cells (HUCPVCs) have been recently reported as a type of mesenchymal stem cell that can accelerate early wound healing in skin defects. However, there are only a limited number of studies that have incorporated these cells into natural scaffolds for dermal tissue engineering. The aim of the present study was to promote angiogenesis and accelerate wound healing by using HUCPVCs and decellularized dermal matrix (DDM) in a rat model of diabetic wounds. The DDM scaffolds were prepared from harvested human skin samples and histological, ultrastructural, molecular and mechanical assessments were carried out. In comparison with the control (without any treatment) and DDM alone group, full thickness excisional wounds treated with HUCPVCs-loaded DDM scaffolds demonstrated an accelerated wound closure rate, faster re-epithelization, more granulation tissue formation and decreased collagen deposition. Furthermore, immunofluorescence analysis showed that the VEGFR-2 expression and vascular density in the HUCPVCs-loaded DDM scaffold treated group were also significantly higher than the other groups at 7 days post implantation. Since the rates of angiogenesis, re-epithelization and formation of granulation tissue are directly correlated with full thickness wound healing in patients, the proposed HUCPVCs-loaded DDM scaffolds may fulfil a role neglected by current treatment strategies. This pre-clinical proof-of-concept study warrants further clinical evaluation. Statement of Significance The aim of the present study was to design a novel tissue-engineered system to promote angiogenesis, re-epithelization and granulation of skin tissue using human umbilical cord perivascular stem cells and decellularized dermal matrix natural scaffolds in rat diabetic wound models. The authors of this research article have been working on stem cells and tissue engineering scaffolds for years. According to our knowledge, there is a lack of an efficient system for the treatment of skin defects using tissue engineering strategy. Since the rates of angiogenesis, re-epithelization and granulation tissue are directly correlated with full thickness wound healing, the proposed HUCPVCs-loaded DDM scaffolds perfectly fills the niche neglected by current treatment strategies. This pre-clinical study demonstrates the proof-of-concept that necessitates clinical evaluations.

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Wound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model

Cited by 332 publications

References 54 publications

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Preparation and characterization of fibrous chitosan-glued phosphate glass fiber scaffolds for bone regeneration

Accelerated wound healing in a diabetic rat model using decellularized dermal matrix and human umbilical cord perivascular cells

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