Wound dressings as growth factor delivery platforms for chronic wound healing

Catanzano, Ovidio; Quaglia, Fabiana; Boateng, Joshua

doi:10.1080/17425247.2021.1867096

Cited by 58 publications

(55 citation statements)

References 198 publications

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“…Further studies are underway on the activity of the CHIT/HEGF composites applied to in vivo wound models for the evaluation of EGF release and its effects on wound progression. [20] Preliminary biological investigations were performed to evaluate the occurrence of any cytotoxic effects and the ability of CHIT/HEGF dressing to support fibroblast viability, with respect to CHIT scaffold, whose cytocompatibility had been previously demonstrated. [12] The in vitro cytotoxicity evaluation is a fast method to provide predictive evidence of material biocompatibility.…”

Section: Resultsmentioning

confidence: 99%

“…By introducing EGF sequence within the backbone of HELP, we obtained a fusion protein (HEGF) maintaining both the EGF bioactivity and the responsivity to a proteolytic environment, such as a wound site. [20] The study reported here focuses on the development of a new composite scaffold that combines the flexibility of the 3D printing technique with the stimuli-induced release ability of a HEGF hydrogel. High-porosity chitosan 3D-printed scaffolds (CHIT) were embedded by enzymatic cross-linking in HEGFenriched HELP matrix and the interaction between these components as well as the release of EGF from the scaffold was evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Elastolytic-sensitive 3D-printed chitosan scaffold for wound healing applications

et al. 2021

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The combination of a chitosan 3D-printed scaffold with a hydrogel matrix containing an elastin-like polypeptide functionalized with the epidermal growth factor (HEGF) was evaluated as a possible strategy to obtain a bioactive platform with stimuli-responsive properties. We designed a chitosan/ HEGF hybrid scaffold and examined the physico-chemical properties and the in vitro behavior when in contact with simulated biological fluids. Primary human dermal fibroblasts (hDFs) were used to test the in vitro cytocompatibility. Overall, these data provide first insights into the integration of HEGF-based hydrogel with 3D-printed scaffolds, contributing towards the rational design of a new smart functional wound dressing.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Elastolytic-sensitive 3D-printed chitosan scaffold for wound healing applications

et al. 2021

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“…Besides, another traditional approach for chronic wound therapy is via fish skin acellular treatment [ 4 ]. This method is considered as one of the significant treatments due to its histological properties that promote cellular regulation and is rich with omega-3 fatty acids to supply to the local tissue [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Wound healing is a dynamic and complex process that initiates the immune response for tissue repair [ 5 ]. Several types of wounds, including vascular ulcers, pressure ulcers, and diabetic ulcers, are primarily categorized as chronic wounds [ 6 ]. The abnormal pathological conditions of chronic wounds lead to a poor healing rate or excessive scar formation after recovery.…”

Section: Introductionmentioning

confidence: 99%

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Masri

Mazlan

Zulkiflee

et al. 2022

IJMS

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Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. Thus, the essential key factor for success in 3D-bioprinting is selecting and developing suitable bioinks to maintain the mechanisms of cellular activity. This crucial stage is vital to mimic the native extracellular matrix (ECM) for the sustainability of cell viability before tissue regeneration. This comprehensive review outlined the application of the 3D-bioprinting technique to develop skin tissue regeneration. The cell viability of human skin cells, dermal fibroblasts (DFs), and keratinocytes (KCs) during in vitro testing has been further discussed prior to in vivo application. It is essential to ensure the printed tissue/organ constantly allows cellular activities, including cell proliferation rate and migration capacity. Therefore, 3D-bioprinting plays a vital role in developing a complex skin tissue structure for tissue replacement approach in future precision medicine.

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“…In addition, clinical researchers have recently explored the efficiency of certain new therapies for DFUs, such as punch grafting, negative pressure wound therapy, and magnetic fields [ [8] , [9] , [10] ]. In addition, considerable research has been conducted for promoting wound healing, among which the local application of growth factors, such as β-fibroblast growth factor (β-FGF), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF), can significantly accelerate wound healing [ [11] , [12] , [13] , [14] , [15] ]. However, there also exist certain challenges that limit the local application of growth factors in chronic wounds, such as high dose requirements, protein instability and its short lifetime in vivo, and high cost [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Dual-functional hybrid quaternized chitosan/Mg/alginate dressing with antibacterial and angiogenic potential for diabetic wound healing

Wang

Yang

Yuan

et al. 2021

Journal of Orthopaedic Translation

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Background Clinic treatment of diabetic foot ulcers (DFUs) is considerably challenging. Impaired wound healing may be caused by poor vascularization and dysfunction of the extracellular matrix, which leads to poor re-epithelialization and increased risk of infection. In this study, we evaluated the treatment potential of a functional dressing comprising quaternized chitosan (hydroxypropyltrimethyl ammonium chloride chitosan) and magnesium (Mg) on DFUs. Methods Dressings were prepared by vacuum freeze-drying. The cellular proliferation, migration, and angiogenesis potential of the functional dressings were determined in vitro. Methicillin-resistant Staphylococcus aureus (MRSA , ATCC43300) and methicillin-resistant Staphylococcus epidermidis 287 (MRSE287) were used to evaluate the antibacterial efficiency of the dressings. Finally, a diabetic rat model with infected wounds was used to further evaluate the effects of functional dressings on the healing of DFUs. Results Functional dressings facilitated the migration of human dermal fibroblasts and human umbilical vein endothelial cells (HUVECs), while also stimulating angiogenesis in HUVECs. Additionally, the functional dressing could effectively eradicate MRSA and MRSE, exhibiting excellent antibacterial ability against drug-resistant bacteria. The results of in vivo microbiological and histological tests demonstrated effective anti-infection ability and wound-healing potential of this functional dressing. Conclusions The dual-functional dressing exhibited wound-healing ability and anti-infection efficiency, demonstrating potential application prospects in DFU treatment. Translational potential of this article As one of the common and serious complications of diabetes, DFUs do not heal easily, causing great suffering to patients. Therefore, improvement in the prognosis of DFUs is a crucial clinical need. The dual-functional dressing prepared in this study was proven to improve the treatment of DFUs, both in vitro and in vivo. Considering its urgent clinical necessity and good biocompatibility of its raw materials, such as alginate, Mg, and chitosan derivatives, this dual-functional dressing presents good prospects for clinical translation.

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Wound dressings as growth factor delivery platforms for chronic wound healing

Cited by 58 publications

References 198 publications

Elastolytic-sensitive 3D-printed chitosan scaffold for wound healing applications

Elastolytic-sensitive 3D-printed chitosan scaffold for wound healing applications

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Dual-functional hybrid quaternized chitosan/Mg/alginate dressing with antibacterial and angiogenic potential for diabetic wound healing

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