Work in progress: Successful transfer of vitrified goat embryos

Yuswiati, E.; Holtz, W.

doi:10.1016/0093-691x(90)90018-o

Cited by 36 publications

(13 citation statements)

References 10 publications

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“…Since the first successful term development from the vitrified goat embryo (Yuswiati and Holtz 1990), many experiments have been conducted on the vitrification of embryos (Traldi et al. 1999; Branca et al.…”

Section: Introductionmentioning

confidence: 99%

Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and Open‐Pulled Straw Method

et al. 2007

Reprod Domestic Animals

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The aim of this study was to investigate the effects of different vitrification solutions [EFS30 or EFS40 contains 30% (v/v) ethylene glycol (EG), 40% (v/v) EG; EDFS30 or EDFS40 contains 15% (v/v) EG and 15% (v/v) dimethyl sulfoxide (DMSO), 20% (v/v) EG and 20% (v/v) DMSO], equilibrium time during vitrification (0.5-2.5 min) and vitrification protocols [one-step straw, two-step straw and open-pulled straw (OPS)] on in vivo development of vitrified Boer goat morulae and blastocysts after embryo transfer. In the one-step straw method, the lambing rates of vitrified embryos in EFS30 (37.5%), EFS40 (40.5%) or EDFS30 (38.2%) group were similar to that of fresh embryos (57.5%) and conventional freezing method (46.7%) when the equilibrium time was 2 min. In the two-step straw method, the highest lambing rate was obtained when embryos were pretreated with 10% EG for 5 min and then exposed to EFS40 for 2 min (51.4%), showing similar lambing rates compared with fresh embryos (56.1%) or the embryos cryopreserved by conventional freezing method (45.2%). In the OPS method, the lambing rate in EFS40, EDFS30 or EDFS40 groups were similar to that (57.1%) of fresh embryos, or to that (46.0%) of embryos cryopreserved by conventional freezing method. The highest lambing rate (51.4%) of the group of OPS was obtained when the embryos were vitrified with EDFS30. In conclusion, either the two-step straw method in which embryos were pretreated in 10% EG for 5 min and then exposed to EFS40 for 2 min, or the OPS method in which embryos were pretreated in 10% EG + 10% DMSO for 30 s and then exposed to EDFS30 for 25 s was a simple and efficient method for the vitrification of Boer goat morulae and blastocysts.

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Section: Introductionmentioning

confidence: 99%

Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and Open‐Pulled Straw Method

et al. 2007

Reprod Domestic Animals

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“…The volume of the ovarian tissue analyzed (V) was calculated by the formula: V (mm 3 ) = S × 0.06, where S corresponds to the sum of all the sections areas (mm 2 ) and 0.06 mm the thickness of the tissue analyzed. For each slice, the follicular density was expressed as the number of follicles per mm 3 of ovarian cortex [23,24].…”

Section: Measurement Of Follicular Densitymentioning

confidence: 99%

“…Until now, vitrification has been adopted to cryopreserve embryos [3,4], oocytes [5][6][7][8][9] and ovarian tissue [10][11][12][13]. Kuwayama reported that vitrification, compared with slow cooling, resulted in high survival rates for all stages of embryo development [14].…”

Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

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“…No: 09098868938 improvement of the vitrification procedure for embryo cryopreservation using the electron microscope grids (Martino et al, 1996), open pulled straws (Vajta et al, 1998), cryoloop (Lane et al, 1999a), cryotop (Kuwayama and Kato, 2000), microdrop method ( Kim et al, 2007;Ocampo et al, 2014), gel loading tip (Tominaga and Hamada, 2001) and a paper container (Kim et al, 2012) with the ultimate aim of preventing injury from intracellular ice formation. One remarkable improvement was the use of a simplified method using a vitrification solution based on ethylene glycol (Kasai et al, 1990;Yushiati and Holtz, 1990;Miyake et al, 1993;Zhu et al, 1993;Guignot et al, 2006) that allow rapid permeation of the cell within 2 min of treatment at 20°C before directly plunging the sample into liquid nitrogen. This method has also been proven effective for rabbit morula (Kasai et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Live birth after transfer of vitrified embryos from superovulated goats

2016

Res. Opin. Anim. Vet. Sci.

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In this study, the viability of vitrified goat embryos recovered from superovulated nondescript Philippine goats were evaluated post-warming by transferring to surrogate does. Of 17 does treated for superovulation, 3 did not respond and 14 responded with a 13.07 mean ovulation rate. An average of 9.7 embryos per doe was collected at different developmental stages. Using a container-less minimum drop size (MDS) method of vitrification, 50 and 64 embryos were exposed to vitrification solution 1 (VS1) containing 10% ethylene glycol in basic medium (BM) consisting of Hepes buffered TCM-199 medium + 20% estrus-doe serum solution for 3 min and 10 min, respectively. Immediately thereafter, the embryos were transferred to 40% EG + 1 M sucrose in BM for 45 Sec before recovering and dropping directly into LN 2. After 3 months of storage, the embryos were warmed in 2 ml of 0.3 M sucrose in BM for 5 min and washed twice before transferring into culture medium. Morphologically normal embryos were then transferred surgically to 6 recipients. Seven kids were born after a normal gestation period with birthweights ranging from 0.78-2.5 kg. The results highlight the usefulness of the MDS method of vitrification using an ethylene glycol based solution in the cryopreservation of goat embryos. That, continuous research effort should be made for optimizing cryopreservation protocols for conservation of animal genetic resources. Keywords: Embryo transfer; live birth; superovulation; vitrification To cite this article: Ocampo MB, JQ Silvestre, VD Viernes and LC Ocampo, 2016. Live birth after transfer of vitrified embryos from superovulated goats. Res. Opin. Anim. Vet. Sci., 6(2): 47-52.

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Work in progress: Successful transfer of vitrified goat embryos

Cited by 36 publications

References 10 publications

Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and Open‐Pulled Straw Method

Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and Open‐Pulled Straw Method

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Live birth after transfer of vitrified embryos from superovulated goats

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