Wool keratin: A novel building block for layer-by-layer self-assembly

Yang, Xiao; Zhang, Hui; Yuan, Xiangwei; Cui, Shuxun

doi:10.1016/j.jcis.2009.04.050

Cited by 32 publications

(20 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reduction and oxidation of disulfide bonds belong to the common methods for keratin isolation. Reduction of keratin involves use of 2‐mercaptoethanol (Balaji et al, ; Fujii & Li, ; Kakkar, Madhan, & Shanmugam, ; Reichl, ; Schrooyen, Dijkstra, Oberthür, Bantjes, & Feijen, ; Tanabe, Okitsu, & Yamauchi, ; Yamauchi, Yamauchi, Kusunoki, Kohda, & Konishi, ), dithiothreitol (DTT), dithioerythritol (Vasconcelos et al, ; Yang, Zhang, Yuan, & Cui, ), thioglycolic acid (Hill et al, ; Zabashta, Kasprova, Senchurov, & Grabovskii, ), glutathione (Schrooyen, Dijkstra, Oberthür, Bantjes, & Feijen, ), salts of hydrocyanic acid (Arai, Sakamoto, Naito, & Takahashi, ), bisulfites (Tonin et al, ), and m‐ bisulphites (Aluigi et al, ; Vasconcelos et al, ) to solubilize the protein. Many keratins can remain trapped within the protective structure, and usually a hydrogen‐bond breaking agent, such as urea, thiourea, transition metal hydroxides, surfactants, and combinations thereof, are included in the extractant to unfold or denature the protein (Torchinsky, ).…”

Section: Solubilization Of Keratinsmentioning

confidence: 99%

Solubilization of keratins and functional properties of their isolates and hydrolysates

2018

View full text Add to dashboard Cite

The keratinous wastes of the textile industry and poultry slaughterhouses may be used as sources of soluble keratins or hydrolysates. This review presents methods for processing raw keratin-based materials into bioproducts with functional and bioactive properties suitable for biomedical, cosmetic, food, and agricultural applications. Soluble keratin can be obtained by thermal treatment in some organic solvents, reduction, or oxidation of the disulfide bonds. Recent studies have shown that keratins contain amino acid sequences with high biological activities such as antioxidant, angiotensin I converting enzyme inhibitory, dipeptidyl peptidase IV inhibitory, and antimicrobial.Peptides containing these sequences may find numerous applications as value-added products in the food industry. More research devoted to development of methods for conversion of animal by-products to novel products is needed. Further technological investigations to create large-scale production methods are also necessary. Practical applicationsThe keratinous wastes represent a problematic by-product to the wool textile industry and poultry slaughterhouses due to the large volumes and their high pollutant load. They are usually incinerated or used for low value purposes such as fertilizers. This review focuses on the trends of application of keratin recovered from animal by-products. Biomaterials for regenerative medicine, cosmetic formulations, and biodegradable food packaging can be obtained as a result of keratin self-assembly. Several peptide sequences released by hydrolysis as bioactive peptides should be studied further for their in vivo antihypertensive, and antidiabetic effects, as well as functional ingredients in foods. K E Y W O R D Sbioactive peptides, functional properties, keratin hydrolysates, keratin isolates

show abstract

Section: Solubilization Of Keratinsmentioning

confidence: 99%

Solubilization of keratins and functional properties of their isolates and hydrolysates

2018

View full text Add to dashboard Cite

show abstract

“…Like many natural-derived biomaterials, keratin-based ones present poor mechanical strength. As a consequence, several approaches for controlling the physical and biological properties have been studied such as, blend systems with natural [58][59][60][61][62] and synthetic [33,63] polymers, use of crosslinking agents [64] and new preparation techniques [31,65,66]. Addition of chitosan to keratin forms strong and flexible films with improved swelling properties.…”

Section: Keratin Films and Coatingsmentioning

confidence: 99%

“…The biocompatibility was also demonstrated by cell adhesion and proliferation onto the films [31]. Recently, Yang et al described the use of keratin for layer-by-layer (LbL) self-assembly [66]. Herein, the film is formed by depositing alternating layers of oppositely charged materials.…”

Section: Keratin Films and Coatingsmentioning

confidence: 99%

The Use of Keratin in Biomedical Applications

Vasconcelos¹,

Cavaco‐Paulo²

2013

CDT

View full text Add to dashboard Cite

Keratins are naturally derived proteins that can be fabricated into several biomaterials morphologies including films, sponges and hydrogels. As a physical matrix, keratin biomaterials have several advantages of both natural and synthetic materials that are useful in tissue engineering and controlled released applications. Like other naturally derived protein biomaterials, such as collagen, keratin possess amino acid sequences, similar to the ones found on extracellular matrix (ECM), that may interact with integrins showing their ability to support cellular attachment, proliferation and migration. The ability of developing biomaterials that mimic ECM has the potential to control several biological processes and this is the case for keratin which has been used in a variety of biomedical applications due to its biocompatibility and biodegradability. This review describes the progress to date towards the use of keratin in the field of wound healing, tissue engineering and drug delivery applications, with highlight to reports of particular relevance to the development of the underlying biomaterials science in this area.

show abstract

“…Keratin hydrolyzates also find use in medicine, mainly for their similarity to human tissue. They are applied most when preparing films for tissue engineering when healing surface wounds (for example, chafes, burns) and as a substance supporting growth of new skin tissue 16,17 . Another medical application consists in making a composite material from polylactic acid and keratin and its utilization as frame for cultivating osteoblastsbone cells 18 .…”

mentioning

confidence: 99%

Characterization of Keratin Hydrolyzates Prepared from Sheep Wool

Mokrejš¹,

Krejčí²,

Sukop³

et al. 2014

Asian J. Chem.

View full text Add to dashboard Cite

Keratins have a very rigid structure, particularly due to cross-linking with disulphide bonds that is highly resistant to action by chemicals and enzymes. In order to obtain soluble keratin (keratin hydrolyzates) it is mostly necessary to apply solutions of acids or alkalis in high concentrations under cooperation of high temperatures or pressure 1,2. In enzymatic processing of keratin, it is appropriate to first pre-process keratin in an alkaline or acid environment or with oxidative or reducing agent. To achieve higher hydrolysis efficiency, more enzymes can be employed simultaneously 3. Keratin hydrolyzates have a wide spectrum of applications. When deciding about concrete use, it is essential to know data on composition of keratin hydrolyzates, their properties (for example, solubility, film-forming and rheological properties, thermal stability) and molecular weight. Keratin hydrolyzates are often employed in agriculture as nitrogenous fertilizers and growth stimulants 4,5. Hydrolyzates have a high nitrogen content (around 15 %) which makes them a high quality and inexpensive source of nitrogen. Moreover, they are biodegradable and preparation of hydrolyzates exhibiting different solubility allows to guide the dosage and release of nitrogen into soil 6,7. Choice of suitable conditions

show abstract

Wool keratin: A novel building block for layer-by-layer self-assembly

Cited by 32 publications

References 59 publications

Solubilization of keratins and functional properties of their isolates and hydrolysates

Solubilization of keratins and functional properties of their isolates and hydrolysates

The Use of Keratin in Biomedical Applications

Characterization of Keratin Hydrolyzates Prepared from Sheep Wool

Contact Info

Product

Resources

About