Wogonoside induces apoptosis in human non-small cell lung cancer A549 cells by promoting mitochondria dysfunction

Luo, Min; Mo, Juanmei; Yu, Qitao; Zhou, Shaozhang; Ning, Ruiling; Zhang, Yu; Su, Cuiyun; Wang, Hongzhi; Cui, Jiandong

doi:10.1016/j.biopha.2018.06.077

Cited by 17 publications

(11 citation statements)

References 18 publications

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“…gigantea extracts has not yet been identified. Wogonoside (wogonin-7-glucuronide, C 22 H 20 O 11 ), a bioactive flavonoid isolated from the roots of Scutellaria baicalensis Georgi, induced apoptosis in human non-small cell lung cancer, A549, cells by promoting mitochondrial dysfunction through the reduction in ATP levels, and the consequent activation of AMP-activated protein kinase/mTOR signaling [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

et al. 2021

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Conventional chemotherapeutic agents for colorectal cancer (CRC) cause systemic side effects and eventually become less efficacious owing to the development of drug resistance in cancer cells. Therefore, new therapeutic regimens have focused on the use of natural products. The anticancer activity of several parts of Calotropis gigantea has been reported; however, the effects of its stem bark extract on inhibition of cancer cell proliferation have not yet been examined. In this study, the anticancer activity of C. gigantea stem bark extract, both alone and in combination with 5-fluorouracil (5-FU), was evaluated. A crude ethanolic extract was prepared from dry, powdered C. gigantea barks using 95% ethanol. This was then partitioned to obtain dichloromethane (CGDCM), ethyl acetate, and water fractions. Quantitative analysis of the constituent secondary metabolites and calotropin was performed. These fractions exhibited cytotoxicity in HCT116 and HT-29 cells, with CGDCM showing the highest potency in both the cell lines. A combination of CGDCM and 5-FU significantly enhanced the cytotoxic effect. Moreover, the resistance of normal fibroblast, HFF-1, cells to this combination demonstrated its safety in normal cells. The combination significantly enhanced apoptosis through the mitochondria-dependent pathway. Additionally, the combination reduced adenosine triphosphate production and increased the production of reactive oxygen species, demonstrating the mechanisms involved in the induction of apoptosis. Our results suggest that CGDCM is a promising anti-cancer agent and may enhance apoptosis induction by 5-FU in the treatment of CRC, while minimizing toxicity toward healthy cells.

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Section: Discussionmentioning

confidence: 99%

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

et al. 2021

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“…Wogonoside (Wg; Fig. Wg also exerts effective anticancer effects in diverse types of cancer cells, including breast cancer [6], colon cancer [7], and non-small-cell lung cancer [8]. Wg also exerts effective anticancer effects in diverse types of cancer cells, including breast cancer [6], colon cancer [7], and non-small-cell lung cancer [8].…”

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confidence: 99%

Retracted: Wogonoside promotes apoptosis in gastric cancer AGS and SGC‐7901 cells through induction of mitochondrial dysfunction and endoplasmic reticulum stress

Xiang

Xiao

et al. 2019

FEBS Open Bio

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Wogonoside (Wg), a natural flavonoid, has anticancer effects against several human cancers. The purpose of the present study was to investigate the antitumor effects and underlying mechanisms of Wg on gastric cancer (GC) cell lines. We report that Wg treatment inhibited cell viability and induced apoptosis in human GC cell lines AGS and SGC‐7901, and also retarded GC tumor growth in xenograft mice in vivo. We also found that the Wg exerted its antitumor effects against GC cells via induction of reaction oxygen species accumulation, mitochondrial dysfunction, and endoplasmic reticulum stress. Furthermore, C/EBP homologous protein knockdown inhibited apoptosis and increased the viability of Wg‐treated GC cells. Our findings may facilitate the development of novel therapeutic agents for the treatment of GC.

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“…The former studies indicated that A549 cells were obviously inhibited after the treatment with different doses of Wogonoside. The half-inhibitory concentration (IC 50 ) value was identi ed to be 46.1 µM [32] .…”

Section: Discussionmentioning

confidence: 99%

The effect of Wogonoside on the expression of pulmonary fibrosis factor in Mycoplasma pneumoniae pneumonia

Wang

Yang

et al. 2020

Preprint

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BACKGROUND Mycoplasma pneumoniae pneumonia (MPP) is an acute respiratory infectious pneumonia with pulmonary interstitial fibrosis. TGF-β1 is well accepted as the central mediator for fibrosis which promotes the formation of tissue fibrosis factor. Qinbaiqingfei pellet (Qinbai) has already been approved as the first effective new traditional Chinese medicine which can delay activities of Mycoplasma pneumoniae (M.pneumoniae) and protect lung epithelial cells in clinical trials. However, the mechanism of Qinbai inhibiting the expression of TGF-β1 is still unclear. METHODS The Chinese herbs in Qinbai were screened by surface plasmon resonance (SPR) and it was deteimined that Scutellaria baicalensis extracts in Qinbai showed the best binding with TGF-β1. Then the active ingredient whicn can be bound with TGF-β1 protein in the solution of Qinbai and Scutellaria baicalensis was isolated and analyzed by UPLC-Q-TOF-MS, which proved the active ingredient was Wogonoside. The affinity constant of Wogonoside and TGF-β1 protein was measured by SPR affinity analysis. A549 cells infected with M.pneumoniae were intervened by Wogonoside, and then the expression of TGF-β1 and Smad3 in A549 cells were analyzed by PCR and western blotting. RESULTS The results showed the bound effect of Scutellaria baicalensis and TGF-β1 was effective. The active ingredients which can be bound with TGF-β1 in the solution of Scutellaria baicalensis and Qinbai were obtained and analyzed to investigate the mechanism of Qinbai inhibiting the expression of TGF-β1. UPLC-Q-TOF-MS results showed that the active ingredients was Wogonoside. SPR affinity analysis showed that the affinity constant was 21.71 µM. Pharmacological experiments revealed that Wogonoside strongly inhibited the expression of TGF-β1 and Smad3 in A549 cells infected by M.pneumoniae. CONCLUSION Wogonoside in Qinbai can be bound with TGF-β1 and down-regulate the expression of lung fibrosis factors TGF-β1 and Smad3. The finding may improve our understanding the molecular mechanism of Qinbai mediating MPP and provide new sights into the future pharmacological investigation of Qinbai.

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Wogonoside induces apoptosis in human non-small cell lung cancer A549 cells by promoting mitochondria dysfunction

Cited by 17 publications

References 18 publications

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

Retracted: Wogonoside promotes apoptosis in gastric cancer AGS and SGC‐7901 cells through induction of mitochondrial dysfunction and endoplasmic reticulum stress

The effect of Wogonoside on the expression of pulmonary fibrosis factor in Mycoplasma pneumoniae pneumonia

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