Wogonoside Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Abstract

Wogonoside has been reported to have anti-inflammatory properties. In this study, we evaluated the effect of wogonoside on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Male BALB/c mice with ALI, induced by intranasal instillation of LPS, were treated with wogonoside 1 h prior to LPS exposure. Mice treated with LPS alone showed significantly increased TNF-α, IL-6, and IL-1β levels in the bronchoalveolar lavage fluid (BALF). When pretreated with wogonoside, the TNF-α, IL-6, and IL-1β levels were significantly decreased. Meanwhile, wogonoside significantly inhibited LPS-induced increases in the macrophage and neutrophil infiltration of lung tissues and markedly attenuated myeloperoxidase activity. Furthermore, wogonoside inhibited the TLR4 expression and the phosphorylation of NF-κB p65, and IκB induced by LPS. In conclusion, our results indicate that wogonoside exhibits a protective effect on LPS-induced ALI via suppression of TLR4-mediated NF-κB signaling pathways.

Platycodin D attenuates acute lung injury by suppressing apoptosis and inflammation in vivo and in vitro

“…In addition, histopathological study also indicated that PLD treatment markedly attenuated the neutrophil infiltration in the lungs. LPS is known to induce the production of several inflammatory and chemotactic cytokines [27]. TNF-α and IL-6 are characterized cytokines involved in the inflammatory process of ALI [28,29].…”

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway

“…In the future, we will study the MAPK signaling pathway. The NF-κB signaling pathway plays an important role in the regulation of the inflammatory and immune responses, inducing the transcription of a series of genes encoding cytokines and chemokines [32]. Under normal conditions, NF-κB is associated with its inhibitor IκB-α in the cytoplasm [33].…”

Wogonoside alleviates inflammation induced by traumatic spinal cord injury by suppressing NF-κB and NLRP3 inflammasome activation

“…In the present study, it was demonstrated that treatment with 25 or 50 mg/kg of wogonoside significantly inhibited inflammation through the suppression of NF-κB/IκB in an SCI rat model. Similarly, Zhang et al (7) reported that wogonoside ameliorates LPS-induced acute lung injury in mice through the suppression of TLR4-mediated NF-κB signaling. The NLRP3 inflammasome promotes the secretion of IL-1β, IL-18 and IL-33, and the production of these factors is critical in the control of pathological infection (24).…”

“…1) has been reported to possess extensive pharmacological activities. In addition to anti-inflammatory, anti-allergy, anti-viral and antipyretic properties, recent studies have demonstrated that wogonoside possesses anti-oxidative and anti-tumor effects, along with cardiovascular protective effects (7,8). Wogonoside is derived from Scutellaria baicalensis, and has exhibited efficacy as an antithrombotic, antihypertensive and antitumor therapeutic, and in the treatment of coronary heart disease (9).…”