Platycodin D attenuates acute lung injury by suppressing apoptosis and inflammation in vivo and in vitro

Tao, Weiwei; Su, Qiang; Wang, Hanqin; Guo, Sisi; Chen, Yanyan; Duan, Jin‐Ao; Wang, Shumin

doi:10.1016/j.intimp.2015.05.005

Cited by 70 publications

(49 citation statements)

References 38 publications

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“…A549 cell seeding into 24-well plates was performed at 1 × 10 6 cells/L. Upon overnight incubation, cells were administered PQ (10 μg/mL) for 2 hours and further treated for 6 hours with RDN (1,2,4,8,16, and 32 μg/mL, respectively), with untreated and AC (100 μM) treated cells as controls. Then, A549 cells and supernatants were collected for subsequent experiments.…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

Reduning injection ameliorates paraquat‐induced acute lung injury by regulating AMPK/MAPK/NF‐κB signaling

Jiang

Zhong

Zhang

et al. 2019

J of Cellular Biochemistry

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Reduning injection (RDN), a patented Chinese medicine, is broadly used for common cold and lung infection in clinic, but the mechanism underlying its effects on inflammation-related pulmonary injury remains unclear. Paraquat (PQ, bolus 15 mg/kg dose, ip) was administered for acute lung injury induction in mice, which were orally administered dexamethasone (2 mg/kg) or RDN (50 and 100 mg/kg/day) for 5 days. After treatment, plasma and lung tissue samples from the euthanized animals were obtained and analyzed by histological, biochemical and immunoblot assays. Histological observation demonstrated RDN alleviated PQ-induced lung damage. Meanwhile, RDN suppressed myeloperoxidase (MPO) activity, reduced the wet/dry (W/D) ratio and decreased the amounts of total leukocytes and neutrophils. Treatment also markedly decreased the amounts of malondialdehyde, MPO, and inflammatory cytokines while increasing superoxide dismutase activity in comparison with the PQ group. In immunoblot, RDN blocked the phosphorylation levels of adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), JNK, ERK, p38, inhibitor of nuclear factor κB kinase and nuclear factor-κB (NF-κB) in lung tissue specimens in PQ-challenged animals, which was further verified in vitro. The above data indicated protective effects for RDN in PQ-induced lung damage, possibly through inhibition of the AMPK/MAPK/NF-κB pathway. K E Y W O R D SAMPK, MAPK, NF-κB, paraquat, pulmonary injury, Reduning injection

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Section: Cell Culture and Treatmentmentioning

confidence: 99%

Reduning injection ameliorates paraquat‐induced acute lung injury by regulating AMPK/MAPK/NF‐κB signaling

Jiang

Zhong

Zhang

et al. 2019

J of Cellular Biochemistry

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“…For instance, the triterpenoid saponins are major constituents of Hedera helix , which is used for lung inflammation (Gepdiremen et al ., 2005; Hocaoglu et al ., 2012). Platycodin D (44), a triterpenoid saponin from Platycodon grandiflorum , also showed inhibitory action against ALI (Tao et al ., 2015). This compound was found to inhibit the expression of NF-κB, caspase-3 and Bax.…”

Section: The Inhibition Of Plant Constituents Against In Vivo Animal mentioning

confidence: 99%

Therapeutic Potential of Medicinal Plants and Their Constituents on Lung Inflammatory Disorders

Kim

Lim

Kwon

2017

Biomolecules & Therapeutics

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Acute bronchitis and chronic obstructive pulmonary diseases (COPD) are essentially lung inflammatory disorders. Various plant extracts and their constituents showed therapeutic effects on several animal models of lung inflammation. These include coumarins, flavonoids, phenolics, iridoids, monoterpenes, diterpenes and triterpenoids. Some of them exerted inhibitory action mainly by inhibiting the mitogen-activated protein kinase pathway and nuclear transcription factor-κB activation. Especially, many flavonoid derivatives distinctly showed effectiveness on lung inflammation. In this review, the experimental data for plant extracts and their constituents showing therapeutic effectiveness on animal models of lung inflammation are summarized.

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“…P. grandiflorum is a traditional Chinese medicine used to treat cough and phlegm, sore and hoarse throat, and lung abscess . Furthermore, it can inhibit hepatic lipogenesis , ameliorate cisplatin‐induced nephrotoxicity , function as an antioxidant , and attenuate acute lung injury according to the results of recent studies. These pharmacological activities have mainly been attributed to the saponins in P. grandiflorum , particularly platycodin D. However, P. grandiflorum also contains various polyacetylenes including lobetyol, lobetyolin, and lobetyolinin .…”

Section: Introductionmentioning

confidence: 99%

“…P. grandiflorum is a traditional Chinese medicine used to treat cough and phlegm, sore and hoarse throat, and lung abscess [2][3][4][5][6]. Furthermore, it can inhibit hepatic lipogenesis [7], ameliorate cisplatin-induced nephrotoxicity [8], function as an antioxidant [9], and attenuate acute lung injury [10] Abbreviations: HMBC, heteronuclear multiple bond correlation; HR-TOF-MS, high-resolution electrospray ionization time-of-flight mass spectrometry; HSCCC, high-speed counter-current chromatography Conflict of interest: No conflict of interest exists in the submission of this manuscript.…”

Section: Introductionmentioning

confidence: 99%

Application of high‐speed counter‐current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum

Bao

Liu

Zhang

et al. 2017

J of Separation Science

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Three polyacetylenes were isolated and purified from Platycodon grandiflorum A. DC for the first time by high-speed counter-current chromatography using a two-phase solvent system composed of hexane/ethyl acetate/methanol/water (1:31:1:31, v/v/v/v) and high-performance liquid chromatography with an Agilent ZORBAX® SB-C18 column (4.6 mm × 150 mm, 5 μm). After separation by high-speed counter-current chromatography and high-performance liquid chromatography, we obtained 3.5 mg of platetyolin A, 4.1 mg of platetyolin B, and 18.1 mg of lobetyolin with purities of 97.2, 96.7, and 96.9%, respectively. The purity of each compound was assessed by high-performance liquid chromatography and the chemical structures were evaluated by high-resolution electrospray ionization time-of-flight mass spectrometry and one- and two-dimensional NMR spectroscopy. Among the isolated compounds, platetyolin A and platetyolin B are newly reported compounds.

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Platycodin D attenuates acute lung injury by suppressing apoptosis and inflammation in vivo and in vitro

Cited by 70 publications

References 38 publications

Reduning injection ameliorates paraquat‐induced acute lung injury by regulating AMPK/MAPK/NF‐κB signaling

Reduning injection ameliorates paraquat‐induced acute lung injury by regulating AMPK/MAPK/NF‐κB signaling

Therapeutic Potential of Medicinal Plants and Their Constituents on Lung Inflammatory Disorders

Application of high‐speed counter‐current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum

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