Wnt11 Functions in Gastrulation by Controlling Cell Cohesion through Rab5c and E-Cadherin

Ulrich, Florian; Krieg, Michael; Schötz, Eva-Maria; Link, Vinzenz; Castanon, Irinka; Schnabel, Viktor; Taubenberger, Anna; Müeller, Daniel J.; Puech, Pierre-Henri; Heisenberg, Carl‐Philipp

doi:10.1016/j.devcel.2005.08.011

Cited by 286 publications

(266 citation statements)

References 38 publications

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“…Wnt11 regulates the E-cadherin-mediated cohesion of the mesoendoderm progenitors (9). In this process, Rab5, one of the key regulators of early endocytosis and intracellular trafficking, mediates Wnt11 activity.…”

Section: Discussionmentioning

confidence: 99%

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Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord

Kida

Sato

Miyasaka

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Convergent extension (CE) movement of cells is one of the fundamental processes that control the organized morphogenesis of tissues and organs. The molecular events connecting the noncanonical Wnt pathway and CE movement, however, are not well understood. We show that subcellular localization of Daam1, an essential component of noncanonical Wnt signaling, changes dynamically during notochord formation. In the early phases, Daam1 complexes with EphB receptors and Disheveled 2. This complex is incorporated into endocytic vesicles in a dynamin-dependent manner, thereby resulting in the removal of EphB from the cell surface with subsequent switching of cell adhesiveness. In the next step, Daam1 colocalizes with the actin cytoskeleton to induce morphological extension of cells. We elucidate the molecular mechanism underlying the CE movement of notochord cells with Daam1 as a dynamic coordinator of endocytosis and cytoskeletal remodeling.Daam ͉ Eph ͉ Wnt ͉ planar cell polarity

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Section: Discussionmentioning

confidence: 99%

“…Wnt11, which acts in the noncanonical Wnt cascade, is involved in this process, controlling the cohesion of mesoendodermal cells through the Rab5c-mediated endocytosis of E-cadherin (9).…”

mentioning

confidence: 99%

Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord

Kida

Sato

Miyasaka

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…It was recently reported that Wnt7a overexpression in the neural tube results in defective adherens junctions, with subsequent abnormalities in spinal neurulation (Shariatmadari et al, 2005). During zebrafish gastrulation, Wnt11 is required to modulate the cell surface localization of E-cadherin via a pathway involving the small GTPase Rab5, which regulates endocytosis (Ulrich et al, 2005). Signaling by another important growth factor TGF␤ 1 , leads to removal of E-cadherin from cell contacts, presumably by affecting the phosphorylation status of ␤-catenin (Vogelmann et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Wingless signaling modulates cadherin-mediated cell adhesion inDrosophilaimaginal disc cells

Wodarz

Stewart

Nelson

et al. 2006

Journal of Cell Science

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Armadillo, the Drosophila homolog of β-catenin, plays a crucial role in both the Wingless signal transduction pathway and cadherin-mediated cell-cell adhesion, raising the possibility that Wg signaling affects cell adhesion. Here, we use a tissue culture system that allows conditional activation of the Wingless signaling pathway and modulation of E-cadherin expression levels. We show that activation of the Wingless signaling pathway leads to the accumulation of hypophosphorylated Armadillo in the cytoplasm and in cellular processes, and to a concomitant reduction of membrane-associated Armadillo. Activation of the Wingless pathway causes a loss of E-cadherin from the cell surface, reduced cell adhesion and increased spreading of the cells on the substratum. After the initial loss of E-cadherin from the cell surface, E-cadherin gene expression is increased by Wingless. We suggest that Wingless signaling causes changes in Armadillo levels and subcellular localization that result in a transient reduction of cadherin-mediated cell adhesion, thus facilitating cell shape changes, division and movement of cells in epithelial tissues.

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“…All confocal images were analysed and quantifi ed using ImageJ v. 1.45 ( http://rsb.info.nih.gov/ij / ). Th e membrane-to-cytosol ratio of respective experiments was calculated on single Z-planes using the methods developed by Janetopoulos et al 39 and Ulrich et al 40 For each of MEFs, 50 staining cells were used for quantitative analysis and statistical analysis ( Fig. 4d ).…”

Section: Methodsmentioning

confidence: 99%

SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane

et al. 2012

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The membrane association of the tumour suppressor phosphatase and tensin homologue (PTEN) is required to oppose the phosphatidylinositol-3-kinase / AKT pathway by dephosphorylation of phosphatidylinositol-3,4,5-triphosphate (PIP3). How cytosolic PTEN interacts with its main substrate, PIP3, localized at the inner face of plasma membrane remains unclear. Here we show that PTEN is covalently modifi ed by SUMO1 at both K 266 and K 254 sites in the C2 domain of PTEN. SUMO1 modifi cation at K 266 located in the CBR3 loop, which has a central role in PTEN membrane association, mainly facilitates cooperative binding of PTEN to the plasma membrane by electrostatic interactions. This results in the downregulation of the phosphatidylinositol-3 kinase / AKT pathway and consequently, suppression of anchorageindependent cell proliferation and tumour growth in vivo . Our data demonstrate a molecular mechanism whereby SUMO1 modifi cation is required for PTEN tumour suppressor function by controlling PTEN membrane association and regulation of the phosphatidylinositol-3 kinase / AKT pathway.

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Wnt11 Functions in Gastrulation by Controlling Cell Cohesion through Rab5c and E-Cadherin

Cited by 286 publications

References 38 publications

Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord

Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord

Wingless signaling modulates cadherin-mediated cell adhesion inDrosophilaimaginal disc cells

SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane

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