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Abstract

An efficient micropropagation protocol from shoot tip explants of Mucuna pruriens, an annual leguminous climber was established. Shoot tip excised from seven days old aseptically grown seedlings were inoculated on Murashige and Skoog (MS 1962) medium containing different concentration of benzyladenine (BA) alone or in combination with an auxins (NAA, IBA, IAA) for multiple shoot induction. BA (2.5 μM) was found to be optimum for inducing maximum shoots (7.67 ± 0.33) per explant. The augmentation of auxins in combination with optimized dose of BA gave antagonistic response towards shoot bud regeneration and resulted in basal callusing.Augmentation of AgNO3 (25 μM) with best cytokinin combination considerably improved the shoot quality with maximum shoot numbers (10.67 ± 0.88) and also gave better elongation (3.97 ± 0.12 cm) at multiplication stage in shoot tip explants. The best condition for rooting was half-strength MS medium solidified with agar and with 0.03 μM indole-3-butyric acid (IBA) producing 4.67 ± 0.33 roots per microshoot after 28 days. After rooting, the plantlets were transferred to plastic pots filled with sterile Soilrite where 90 % grew well and all exhibited normal development.

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

“…Sucrose was shown to play a vital role in improving plant growth and shoot regeneration rate, while glucose and maltose played a minor role in shoot induction. The findings in the current study are consistent with previous studies which reported that sucrose induced shoot formation of Soybean [66], Velvet bean [67], Peach [68], Gotu Kola [16], Pea [69,70], Kris [71], Cucumber [72]. The types of carbohydrate sources, and their concentrations may influence culturing success [63,65].…”