In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Abstract

The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

In Vitro Propagation And Rejuvenation of Senescent Maternal Plant of Ardisia Crenata Var. Bicolor (Primulaceae)

“…The quality of the root induction is affected by the composition and concentration of the media, most species require auxin to induce rooting and shoot promotion 30 , but the root induction rate was decreased with increasing concentration of IAA 31 . Some research showed that IBA was found to be more effective in rooting induction compared to NAA and IAA 25,32 .…”

<i>In vitro</i> regeneration of soybean (a review)

“…al., 2017;Sojková et al, 2016) использован газообразный хлор как стерилизующий агент. Другие авторы использовали этанол: 70% (Soto et al, 2013;Kantayos, Bae, 2019b;Varlamova et al, 2018) и 75% (Aslam et al, 2020), 3-процентную перекись водорода (Zhumagulova, 2014), твин-20 (Raza et al, 2020) и концентрированную соляную кислоту (Mangena, Mokwala, 2019). Длительность стерилизации составляла от 15 минут до 20 часов, в отдельных случаях достигала 48 часов.…”

“…Практически во всех работах по изучению регенерационной способности сои (Sojková et al, 2016;Raza et al, 2019;Aslam et al, 2020) авторы использовали питательную среду на основе среды Мурасиге-Скуга (МС) (Murashige, Skoog, 1962) (табл. 2).…”

“…Максимальная регенерация (80%) была получена на среде 1/2 MС (Aslam et al, 2020). В то же время между культивированием эксплантов на полной среде MС и 1/3 MС значительных различий в уровне регенерации не наблюдали, а разбавление MС в четыре раза привело к более низкому уровню регенерации по сравнению со всеми другими вариантами сред -всего 10% (Aslam et al, 2020). Было установлено, что 3% сахарозы в питательной среде вызывают самую высокую частоту регенерации побегов, чем все другие источники углеводов для всех эксплантов.…”

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use

“…White lupin ( Lupinus albus ) is an economically important legume crop belonging to the family Fabaceae due to its high nutritious value, high protein and low oil contents [ 22 ]. It gained a lot of research attention due to its significant role in improving nutrient mobilization, soil exploration and fertility, and nitrogen-fixing ability [ 23 ].…”