Within-flock variations of Campylobacter loads in caeca and on carcasses from broilers

Prevalence, seasonality and antibiotic susceptibility of thermophilic Campylobacters in ceca and carcasses of poultry birds in the live-bird market

“…Our findings on the prevalence of Campylobacter, based on cecal samples, corresponds well with those reported by Stern and Robach (2003) with an average population of 4.6 log 10 CFU/g in 1995 and an average population of 5.17 log 10 CFU/g in 2001 (Stern and Robach, 2003). These findings are also in agreement with values reported by Berrang et al (2000) and Hansson et al, (2010).…”

“…Then one loopful of enrichment broth was streaked onto mCCDA agar plates (ISO, 10272-1;Stoyanchev et al, 2007;Habib et al, 2011). For qualitative analysis, about 0.5 g of caecal contents were directly plated on mCCDA plates (Hansson et al, 2010) Both sets of mCCDA plates were then incubated at 42 ± 1°C for 24 h under microaerobic conditions using McIntosh and Filde's anaerobic jar (Hi-Media, IndiaAnaerobic System Mark VI, LE013). Plates were inspected to detect the presence of colonies presumed because of their characteristics of Campylobacter.…”

“…Then a 10-fold serial dilution in peptone water was prepared. One milliliter from each dilution was then plated on mCCDA plates (Hansson et al, 2010).…”

Risk based microbiological criteria for Campylobacter in broiler meat in the European Union

“…On the basis of data (e.g. Hansson et al, 2010) and risk assessment models (Nauta et al, 2007), we implicitly assume that in these batches the within-batch prevalence is 100% at the carcass level. However, if the mean concentration is low and/or the standard deviation of concentrations is large, some carcasses may be contaminated with a concentration that is below the detection limit, or even so low that there may actually be no Campylobacters on the carcass at all.…”

Risk-based microbiological criteria for Campylobacter in broiler meat: A comparison of two approaches

“…The inclusion of other data sets (like e.g. Hansson, Pudas, Harbom, & Olsson Engvall, 2010;Stern et al 2007) would have required additional methods for data analyses, and other methods and assumptions in the uncertainty analysis. Adding these would not have contributed to the clarity of this paper and would not have changed its main messages.…”