“…In general, a larger or less variable response is recorded when the subject is asked to read a book or attend to the stimuli (e.g., count the stimuli) rather than simply sit with eyes open. 13,14 It is generally held that the N1-P2 response is attenuated by sleep (which is therefore to be avoided when testing), but many studies have shown a more complex picture, in which response latencies increase during the stages of sleep but the amplitude of the response may either increase or decrease, depending on the sleep stage. 15,16 Unless the stage of sleep is assessed and controlled, it is prudent to employ a test protocol in which sleep is avoided.…”