WITHDRAWN: Development of a Combined Molecular Diagnostic and DNA Fingerprinting Technique for Rice Bacteria Pathogens in Africa

Onasanya, A.; Basso, A.; Somado, E.; Gasore, Elie R.; Nwilene, F. E.; Ingelbrecht, Ivan; Lamo, J.; Wydra, Kerstin; Ekperigin, M. M.; Langa, M.; Oyelakin, Oladele; Séré, Y.; Winter, Stephan; Onasanya, R.O.

doi:10.3923/biotech.2010.89.105

Cited by 17 publications

(16 citation statements)

References 32 publications

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“…oryzae (Nagaraju et al, 2007;Das et al, 2014). In 1884, the farmers of Japan, growing rice noticed the disease first (Onasanya et al, 2010). After that the disease was also noticed from various parts of Asia, northern Australia, Africa and USA (Shanti et al, 2001;Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

Sabar¹,

Bibi²,

Farooq³

et al. 2016

Afr. J. Biotechnol.

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Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has now become one of major rice diseases. During recent years, its occurrence has increased in Pakistan, particularly in 'Kallar tract' which is well-known for quality rice cultivation. Development of new resistant varieties by utilizing the available resistant gene-pool is most reliable breeding technique for the plant breeders. The present study was conducted to explore the genetic resources regarding presence and absence of BLB resistance genes Xa4, xa5 and Xa21 through DNA marker technology. Effectiveness of Xa21 followed by xa5 and Xa7 genes has been reported to confer more resistance level for BLB in traditional rice belt of Pakistan. For this purpose, eighty (80) rice genotypes comprising of diverse origin including three isogenic lines viz., IRBB4 (carrying Xa4), IRBB5 (carrying xa5) and IRBB21 (carrying Xa21) as positive resistant gene checks and IR24 (carrying none of these) as negative gene check were genotyped. DNA fingerprinting results indicated the presence of Xa4 gene in 41 entries, while 14 lines were positive for xa5 gene. Only one local line was carrying Xa21 gene along with Xa4. Thus, the present study will not only be helpful for rice breeders to develop new rice varieties carrying disease resistant genes, but will also provide the donors to incorporate / pyramid disease resistant genes in popular rice varieties like Super Basmati and Basmati 515 which are susceptible to BLB disease.

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Section: Introductionmentioning

confidence: 99%

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

Sabar¹,

Bibi²,

Farooq³

et al. 2016

Afr. J. Biotechnol.

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“…oryzae has now been confirmed in Uganda (Onasanya et al, 2010). However, little is known about the variability of local Xanthomonas oryzae pv.oryzae pathogen populations (Lamo, 2009).…”

Section: Bacterial Leaf Blight (Blb) Of Rice Caused Bymentioning

confidence: 99%

Magnitude of genotype x environment interaction for bacterial leaf blight resistance in rice growing areas of Uganda

Lussewa¹,

Edema²,

Lamo³

2016

Afr. Crop Sci. J.

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“…The molecular studies aimed to determine diversity among the pathogen isolates, polymorphism of certain of its genes and also genetic comparison of P. aeruginosa isolates from different hosts and environments (Kiewitz and Tummler, 2000;Martin et al, 1999;Rumiy et al, 2001;Onasanya et al, 2010).…”

Section: Sourcementioning

confidence: 99%

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

Raafat¹,

Ali-Tammam²,

Ali³

2016

Afr. J. Microbiol. Res.

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Aim & Objectives: The present study was aimed to determine phenotypic characterization of virulent Pseudomonas aeruginosa and its Type 3 Secretion system from Surat region. Methods: 125 clinical Pseudomonas aeruginosa were procured from different laboratories of Surat and 53 environment Pseudomonas aeruginosa isolated from 125 various environment and vegetable samples. Antimicrobial susceptibility testing was performed by Kirby Bauer Disc Diffusion method. The phenotypic screening evaluated for six soluble virulence factors (amylase, Caseinase, gelatinase, haemolysins, lecithinase, and lipase). Biofilm formation was detected by Tube method and Congo red agar method. Four virulence genes (exoS, exoT, exoU, exoY) were screened in selected isolates by PCR. Results: A high percentage of resistance rates (89.60% to amoxyclav and 88% to Cefuroxime) were observed amongst most of the isolates. Soluble enzymes were expressed in variable proportions (Gelatinase 96.80%, Lipase 96%, Lecithinase 88%, and Caseinase 88%). Tube method was considered to be superior to congo red agar method. The most frequent virulent gene detected was exoU (100%). Conclusions: Clinical and environmental virulent Pseudomonas aeruginosa harbour various genes that represent type 3 secretary systems and have high level of resistance that may result in to therapeutic failure.

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WITHDRAWN: Development of a Combined Molecular Diagnostic and DNA Fingerprinting Technique for Rice Bacteria Pathogens in Africa

Cited by 17 publications

References 32 publications

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

Magnitude of genotype x environment interaction for bacterial leaf blight resistance in rice growing areas of Uganda

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

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