WITHDRAWN: A three-dimensional bioprinting technique, based on a gelatin/alginate hydrogel, for the tissue engineering of hair follicle reconstruction

Kang, Deni; Liu, Zhen; Qian, Chuanmu; Huang, Junfei; Zhou, Yi; Mao, Xiaoyan; Qu, Qian; Liu, Bingcheng; Wang, Jin; Wang, Yilin; Hu, Zhiqi; Huang, Wenhua; Miao, Yong

doi:10.1016/j.ijbiomac.2021.09.014

Cited by 1 publication

(1 citation statement)

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“…Moreover, although pre-vascularized collagen fibers, formed via foreign body reaction [35], and pre-vascularized layerby-layer microtissues [11] enhance blood perfusion in regenerative tissues, they require complex and relatively inefficient preparation processes. In contrast, a 3D-bioprinted scaffold [36] can better simulate the HF microenvironment, however, is incapable of simulating the associated macroenvironment. Accordingly, to achieve effective hair regeneration, we raised a scalable and highthroughput approach for DPC/PRP-laden microcarriers integrated with EPC-laden hydrogel to mimic the physiological behaviors of DPC, as well as the HF micro-and macroenvironment, while improving the efficiency and output of the preparation process.…”

Section: Preparation and Characterization Of Hydrogelsmentioning

confidence: 99%

Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering

et al. 2022

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Background Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment. However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells. Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip. Results The ECM-mimicking hydrogels used for IGMs exhibited surface nano-topography and high porosity. Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio. Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase. DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group). Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05). Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice. The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each). Conclusion Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.

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Section: Preparation and Characterization Of Hydrogelsmentioning

confidence: 99%