Wine lees valorization: Biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis

Dimou, Charalampia; Kopsahelis, Nikolaos; Papadaki, Aikaterini; Papanikolaou, Séraphim; Kookos, Ioannis K.; Mandala, Ioanna; Koutinas, Apostolis

doi:10.1016/j.foodres.2015.02.020

Cited by 88 publications

(68 citation statements)

References 46 publications

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“…As a matter of fact, C. necator was found to grow and produce the biopolymer from diverse carbon sources 19,[27][28][29][30][31][32] . Among winery waste, wine lees were used as supplementary medium 33 and enzyme pre-treated GP (saccharified) were used as carbon source 34 . However, acidified pre-treated GP was never tested as the substrate for the biotechnological production of PHAs.…”

Section: Phas Productionmentioning

confidence: 99%

Towards multi-purpose biorefinery platforms for the valorisation of red grape pomace: production of polyphenols, volatile fatty acids, polyhydroxyalkanoates and biogas

et al. 2016

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The development of a multi-purpose four step-cascading biorefinery scheme for the valorization of red grape pomace (GP) was proposed. The consecutive processes were respectively dedicated to (a) the recovery of polyphenols by supercritical CO2 extraction, (b) the production of volatile fatty acids (VFAs) by anaerobic acidogenic digestion, (c) the exploitation of produced VFAs as the precursors for the biotechnological production of polyhydroxyalkanoates (PHAs) and (d) the production of a CH4-rich biogas by the anaerobic digestion of solid leftovers from the acidogenic process. More than 2.7 g of total polyphenols (as gallic acid equivalents) per 100 g of dry matter were extracted. A high content of valuable proanthocyanidins occurred in the recovered polyphenolic fraction. The dephenolized GP was anaerobically digested under batch acidogenic conditions, obtaining about 20 g L-1 of total VFAs in the liquid effluent. The latter matrix was employed to feed a pure culture of a Cupriavidus necator strain, which was induced to produce and store PHAs under nitrogen-limiting conditions. The process was carried out in 0.5 L-shake flasks by using a two-step production approach. In particular, pre-grown biomass was fed with different concentrations of the acidic effluent (20 or 40% v/v) in two sequential batch processes. Poly(3-hydroxybutyrate) was accumulated up to 63% of the cells dry weight when pre-grown biomass was fed with 40% of the acidic effluent. No inhibitory effects due to non-VFA compounds occurring in the actual acidogenic effluent were observed. Finally, the anaerobic digestion of the exhausted solid fraction from the acidogenic process allowed obtaining 113 mL of biomethane per gram of fed volatile solids. © The Royal Society of Chemistry 2016

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Section: Phas Productionmentioning

confidence: 99%

Towards multi-purpose biorefinery platforms for the valorisation of red grape pomace: production of polyphenols, volatile fatty acids, polyhydroxyalkanoates and biogas

et al. 2016

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“…Botella et al [85] used partly pearled wheat grains as substrate for the cultivation of A. awamori, and the hydrolysate obtained through this system was used to produce ethanol and, more interesting biodegradable plastic PHB (polyhydroxybutyrate). Recently, Dimou et al [86] purposed a biorefinary concept in which wine lees (a residue from wine industry) were fractionated for the achieving of antioxidants, tartrate, and ethanol, and the residual stream enriched in yeast cells was used as fermentation supplements for PHB production using a strain of Cupriavidus necator. The enzymatic hydrolysis of the yeast cells was performed by an enzyme consortia produced by SSF of Aspergillus oryzae.…”

Section: Production Of Generic Fermentation Feedstock Mediummentioning

confidence: 99%

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

et al. 2017

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Abstract:The establishment of a bioeconomy era requires not only a change of production pattern, but also a deep modernization of the production processes through the implementation of novel methodologies in current industrial units, where waste materials and byproducts can be utilized as starting materials in the production of commodities such as biofuels and other high added value chemicals. The utilization of renewable raw resources and residues from the agro-industries, and their exploitation through various uses and applications through technologies, particularly solid-state fermentation (SSF), are the main focus of this review. The advocacy for biocatalysis in green chemistry and the environmental benefits of bioproduction are very clear, although this kind of industrial process is still an exception and not the rule. Potential and industrial products, such as biocatalysts, animal feed, fermentation medium, biofuels (biodiesel, lignocelulose ethanol, CH 4 , and H 2 ), pharmaceuticals and chemicals are dealt with in this paper. The focus is the utilization of renewable resources and the important role of enzymatic process to support a sustainable green chemical industry.

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“…The strain was originally isolated and purified by Wang et al [19], from a soy sauce industry (Amoy Food Ltd., Hong Kong, China). Storage, maintenance, sporulation, and inoculum preparations of the fungal strain A. oryzae have been described in previous studies [18,20].…”

Section: Microorganisms and Growth Mediamentioning

confidence: 99%

“…Fungal spore suspension of Aspergillus oryzae was used to inoculate the SSF so as the inoculum spore concentration was approximately 2 × 10 6 spores/mL and the moisture content of the substrate was adjusted at 65% (w/w, db) [18,20]. All SSFs were incubated at 30 • C for up to 48 h. Then, the fermented solids were suspended in sterilised water, macerated using a kitchen blender and filtered in order to obtain the crude enzyme consortia suspension, which was subsequently used in soybean cake hydrolysis.…”

Section: Crude Enzyme Production By Solid State Fermentationmentioning

confidence: 99%

“…The fungal strain A. oryzae was selected in the present study due to the efficient production of proteolytic enzymes on various substrates [18,20,27]. The initial moisture content and pH value have been optimised in previous studies [18,20], thus the SSF on soybean cake was employed at an initial moisture content of 65% and uncontrolled pH.…”

Section: Enzymatic Hydrolysis Of Soybean Cakementioning

confidence: 99%

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Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582

et al. 2017

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Fumaric acid is a platform chemical with many applications in bio-based chemical and polymer production. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL 2582 was analysed using image analysis software and the impact on fumaric acid production was evaluated. Batch experiments were carried out in shake flasks using glucose as carbon source. The highest fumaric acid yield of 0.84 g/g total sugars was achieved in the case of dispersed mycelia with a final fumaric acid concentration of 19.7 g/L. The fumaric acid production was also evaluated using a nutrient rich feedstock obtained from soybean cake, as substitute of the commercial nitrogen sources. Solid state fermentation was performed in order to produce proteolytic enzymes, which were utilised for soybean cake hydrolysis. Batch fermentations were conducted using 50 g/L glucose and soybean cake hydrolysate achieving up to 33 g/L fumaric acid concentration. To the best of our knowledge the influence of R. arrhizus morphology on fumaric acid production has not been reported previously. The results indicated that dispersed clumps were more effective in fumaric acid production than pellets and renewable resources could be alternatively valorised for the biotechnological production of platform chemicals.

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Wine lees valorization: Biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis

Cited by 88 publications

References 46 publications

Towards multi-purpose biorefinery platforms for the valorisation of red grape pomace: production of polyphenols, volatile fatty acids, polyhydroxyalkanoates and biogas

Towards multi-purpose biorefinery platforms for the valorisation of red grape pomace: production of polyphenols, volatile fatty acids, polyhydroxyalkanoates and biogas

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582

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