Wilms' tumor (WT1) (±KTS) variants decreases the progesterone secretion of bovine ovarian theca cells

Wang, X.; Meng, Kai; Wang, Y.; Zhao, Yong; Lin, Feifei; Liu, X.; Zhang, Yong; Quan, Fusheng

doi:10.1016/j.domaniend.2020.106521

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…In the ovary, use the steroidogenic genes (CYP19A1, CYP17A1, and CYP11A1) to convert cholesterol to estrogens 20 . In the current study, the mRNA and proteins expressions of 17β-HSD and CYP17A1 (the key gene of P4 and androstenedione secretion) 29 were upregulated in RPG-fed cows compared with CT cows, although the mRNA and proteins expressions of 3β-HSD and StAR were downregulated and the mRNA expressions of CYP11A1 and CYP19A1 were not affected. These results suggested that the synthesis of gonadotropins may be accelerated and that the expressions of strategic factors were stimulated in cows in the RPG group compared to those in the CT group.…”

Section: Discussionmentioning

confidence: 49%

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum

Wang

Jin

et al. 2023

Sci Rep

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This study was conducted to determine the response of the reproductive hormones and the mTOR/AKT/PI3K pathway in the ovaries of postpartum dairy cows with dietary rumen-protected glucose (RPG). Twelve Holstein cows were randomly assigned to two groups (n = 6/group): the control group (CT) and the RPG group. Blood samples were collected on d 1, 7, and 14 after calving for the gonadal hormone assay. The expression of the gonadal hormones receptors and PI3K/mTOR/AKT pathways were detected using RT-PCR and Western blot. The RPG addition increased the plasma LH, E2, and P4 concentrations on d 14 after calving and upregulated the mRNA and protein expressions of the ERα, ERβ, 17β-HSD, FSHR, LHR, and CYP17A1 but downregulated StAR expression. Immunohistochemical analysis identified higher expressions of the FSHR and LHR in the ovaries of RPG-fed cows compared to CT cows. Furthermore, the protein expressions of p-AKT/AKT and p-mTOR/mTOR were significantly increased in the ovaries of RPG-fed cows compared to the CT group, but the addition of RPG did not alter the protein expression of p-PI3K/PI3K. In conclusion, the current results indicated that dietary RPG supplementation regulated gonadotropin secretion and stimulated expression of hormone receptors and the mTOR/AKT pathway in the ovaries of early postpartum dairy cows. RPG may be beneficial for the recovery of ovarian activity in post-calving dairy cows.

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Section: Discussionmentioning

confidence: 49%

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum

Wang

Jin

et al. 2023

Sci Rep

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“…To evaluate the cellular response of CAL-62 and BHT-101 to ART, CCK-8 cell proliferation assay was performed on 96-well plates bearing CAL-62 and BHT-101 cells treated with or without different concentrations (50, 100, 150, and 200 μM) of ART for 24 h or 48 h using a previously described method [ 11 ]. Flow cytometry (Beckman Coulter, CA, USA) was used to determine the cell cycle phase after treatment with 100 μM ART for 48 h, and the data were analyzed using MODFIT software (Version 5.0, Verity Software House Inc., ME, USA).…”

Section: Methodsmentioning

confidence: 99%

Pyrvinium pamoate can overcome artemisinin’s resistance in anaplastic thyroid cancer

2021

BMC Complement Med Ther

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Background Anaplastic thyroid carcinoma is a highly lethal subtype of thyroid cancer without effective therapies. Drug resistance in anaplastic thyroid carcinoma poses a significant problem. Although artemisinin exerts antitumor effects, but its efficacy in anaplastic thyroid carcinoma is unknown. Methods We used RNA sequencing to identify differentially expressed genes. Next, we determined the cause of ART resistance by testing the expression and activity of β-catenin, and enhanced ART activity with a WNT signaling inhibitor. Results Artemisinin suppressed the growth of BHT-101 but not human thyroid anaplastic carcinoma (CAL-62) cells. The mechanism of artemisinin resistance in CAL-62 was associated with the aberrant activation of WNT signaling. Pyrvinium pamoate, an inhibitor of WNT signaling, was used to overcome ART resistance in CAL-62 cells. The combination of artemisinin and pyrvinium pamoate suppressed the growth of CAL-62 cells and induced the apoptosis. Conclusions Our study is the first to prove the efficacy of ART as monotherapy or in combination with PP in the management of anaplastic thyroid cancer, and that the inhibition of WNT signaling may overcome ART resistance.

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“…There is only one Wt1 homologue in tetrapod species, while there are two paralogs in teleost fish, namely Wt1a and Wt1b [37]. Especially, Wt1 (tetrapod Wt1; fish Wt1a and Wt1b) includes the variants of Wt1 (+KTS) and Wt1 (-KTS), being different in three amino acids (aa), e.g., KTS (lysine, threonine, and serine) [38][39][40]. In animals lacking the Wt1 (+KTS) variant, podocyte function was disturbed and sex reversal occurred, while +KTS variants were considered important regulators of the Sry gene in the sex determination pathway [30,39].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Two Gonadal Genes, zar1 and wt1b, in Hermaphroditic Fish Asian Seabass (Lates calcarifer)

Cui,

Zhu,

Ban

et al. 2024

Animals

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Zygote arrest-1 (Zar1) and Wilms’ tumor 1 (Wt1) play an important role in oogenesis, with the latter also involved in testicular development and gender differentiation. Here, Lczar1 and Lcwt1b were identified in Asian seabass (Lates calcarifer), a hermaphrodite fish, as the valuable model for studying sex differentiation. The cloned cDNA fragments of Lczar1 were 1192 bp, encoding 336 amino acids, and contained a zinc-binding domain, while those of Lcwt1b cDNA were 1521 bp, encoding a peptide of 423 amino acids with a Zn finger domain belonging to Wt1b family. RT-qPCR analysis showed that Lczar1 mRNA was exclusively expressed in the ovary, while Lcwt1b mRNA was majorly expressed in the gonads in a higher amount in the testis than in the ovary. In situ hybridization results showed that Lczar1 mRNA was mainly concentrated in oogonia and oocytes at early stages in the ovary, but were undetectable in the testis. Lcwt1b mRNA was localized not only in gonadal somatic cells (the testis and ovary), but also in female and male germ cells in the early developmental stages, such as those of previtellogenic oocytes, spermatogonia, spermatocytes and spermatids. These results indicated that Lczar1 and Lcwt1b possibly play roles in gonadal development. Therefore, the findings of this study will provide a basis for clarifying the mechanism of Lczar1 and Lcwt1b in regulating germ cell development and the sex reversal of Asian seabass and even other hermaphroditic species.

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Wilms' tumor (WT1) (±KTS) variants decreases the progesterone secretion of bovine ovarian theca cells

Cited by 6 publications

References 29 publications

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum

Pyrvinium pamoate can overcome artemisinin’s resistance in anaplastic thyroid cancer

Characterization of Two Gonadal Genes, zar1 and wt1b, in Hermaphroditic Fish Asian Seabass (Lates calcarifer)

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