Widening the genetic basis of virus resistance in tomato

Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato: exploitation of resistance to viruses

“…1). In a previous work developed by our group, the tomato line NE-1 was crossed as female parent to some plants of S. peruvianum PI 126944 and three interspecific and self-incompatible hybrids (F1-A, F1-B and F1-E) were obtained by embryo rescue (Picó et al 2002). Three backcross generations to the tomato old variety Fortuna C (FC) were obtained.…”

“…Clonal replicates of the three hybrids developed by Picó et al (2002) were employed in a first inoculation trial (Inoculation trial 1, IT1, Table 1). Inoculation of pseudo-F2, pseudo-F3-BC1 and intercrosses between pseudo-F3-BC1 plants (Inoculation trial 2, IT2) was also carried out (Table 1).…”

“…Isolates used were TYLCV-Mld [ES:72:97] (accession L27708) and TYLCSV-ES[2] (accession L27708), kindly provided by Dr. E. Moriones (Estación Experimental "La Mayora", Málaga), and Dr. E. R. Bejarano (Universidad de Málaga), respectively. Agroinoculation at four true-leaves state was used in IT1, following the methodology described by Picó et al (2002). Plants in IT2 were whitefly-inoculated with Bemisia tabaci Genn.…”

“…From these crosses, three interspecific and self-incompatible hybrids (F1-A, F1-B and F1-E) were obtained by embryo rescue (Picó et al 2002). At present work, a collection of generations derived from these hybrids were developed (Fig.…”

“…radicis-lycopersici (Rowe and Farley 1981). Our group constructed some interspecific hybrids between this accession and tomato, which behaved as resistant to TSWV and TYLCD (Picó et al 2002).…”