Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato: exploitation of resistance to viruses

Abstract

A set of introgression lines from S. peruvianum PI 126944 into the genetic background of cultivated tomato (S. lycopersicum) is being developed. Several generations were derived from three interspecific hybrids previously obtained. A lot of crosses and embryo rescue were required to obtain until the third backcross, due to the high incompatibility existing between tomato and PI 126944. Crosses between F1 plants allowed the obtaining of a pseudo-F2 generation. The same procedure was followed until pseudo-F6 generation. Additional crosses between plants of different generations were made in order to increase progeny. Among a total number of 263 molecular markers tested, 105 resulted polymorphic between tomato and PI 126944. This set of polymorphic markers consisted in 90 Simple Sequence Repeats and 15 Cleaved Amplified Polymorphic Sequences. Generations available were genotyped with these markers, observing a progressive reduction in the S. peruvianum genome in the most advanced. A reduction of incompatibility was achieved as a consequence of the S. peruvianum genome reduction. In addition, S. peruvianum genome was almost completely represented considering the different plants of the most advanced generations, so the set of ILs will be basically developed from them. Tomato yellow leaf curl virus (TYLCV) and Tomato spotted wilt virus (TSWV) resistance was evaluated in some generations, having been successfully introgressed and expressed into tomato background.

The Global Dimension of Tomato Yellow Leaf Curl Disease: Current Status and Breeding Perspectives

“…Despite the high incompatibility, accessions of S. chilense and S. peruvianum have been well utilized as the most resistant sources. Exploitation of TYLCV resistance in S. peruvianum began from the incorporation of the resistance from accession PI 126935 [133] and PI 126944 [113,117,134]. Resistant S. chilense accessions (LA1932, LA1938, LA1960, LA1969, LA1971, LA2779 and LA3473) have been intensively applied into breeding practices [92,114,[135][136][137].…”

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

“…Three interspecific hybrids were obtained between cultivated tomato NE-1 and PI 126944, and a set of introgression lines into the genetic background of tomato is being developed from these ( Picó et al, 2002 ). In some of these generations the resistance level against TYLCV and TYLCSV has been assessed ( Julián et al, 2013 ). Interspecific hybrids between cultivated tomato ( S. lycopersicum ) and S. chilense accessions (i.e., LA1932, LA1938, LA1959, LA1960, LA1963, LA1969, LA1971, LA2779, LA3473, UPV20306, UPV20328, and UPV20329) were made leading to different types of populations ( Picó et al, 1999c , 2002 ; Pérez de Castro et al, 2013 ).…”

Espécies de Ralstonia no Brasil : caracterização fenotípica, molecular, novas fontes de resistência em tomateiro e patogenicidade em cafeeiro

“…No Chile, a espécie S. peruvianum está amplamente disseminada como uma planta invasora em consequência de sua elevada fecundidade (mais sementes por fruto e com florescimento mais precoce) quando comparada com outras espécies do gênero Solanum (Chetelat et al, 2009). Solanum peruvianum é considerada a espécie mais variável entre os parentes selvagens do tomateiro (Julián et al, 2013). É uma espécie predominantemente auto-incompatível.…”

“…É uma espécie predominantemente auto-incompatível. Além disso, seu uso como parental feminino (receptor de pólen) em cruzamentos interespecíficos com acessos de tomateiro tem sido evitado devido à existência de incompatibilidade unilateral (Hogenboom, 1972;Julián et al, 2013). Outra complicação associada com o uso de S. peruvianum no melhoramento do tomateiro tem sido os baixos níveis de compatibilidade de cruzamento com o tomateiro cultivado (S. lycopersicum), demandando, muitas vezes, a necessidade de estratégias de resgate de embrião in vitro (Domínguez et al, 2005).…”