Why are the anionic porphyrins so efficient to induce plant cell death? A structure-activity relationship study to solve the puzzle

Leroy‐Lhez, Stéphanie; Rezazgui, Olivier; Issawi, Mohammad; Elhabiri, Mourad; Calliste, Claude-Alain; Riou, Catherine

doi:10.1016/j.jphotochem.2018.09.050

Cited by 8 publications

(35 citation statements)

References 59 publications

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“…Further analyses, such as the H 2 O 2 content, MDA, MTT, total thiols and microscopy analysis, con rmed fungus stress to the point of dying. Our ndings proved that TPPS, due to its characteristics, could be a valid alternative to classic fungicides (Leroy-Lhez et al 2019).…”

Section: Discussionsupporting

confidence: 57%

“…Indeed, TPPS with an ammonium counterion could be as an excellent antifungal candidate. Moreover, it was demonstrated that TPPS remains negatively charged in a large array of chemical environments, even under acidic pH and does not aggregate in media, allowing it to diffuse through cell walls and membranes more easily (Leroy-Lhez et al 2019;Issawi et al 2019). Therefore, this PS was thought as an excellent candidate for APDT.…”

Section: Discussionmentioning

confidence: 99%

“…With the aim to develop APDT for agriculture applications, TPPS could be a good candidate because of its low toxicity for plants. Moreover, TPPS remains negatively charged in many chemical environments even under acidic pH and does not aggregate in solution, allowing it to easily permeate cells through cell walls and membranes (Leroy-Lhez et al 2019;Issawi et al 2019). Therefore, we hypothesized that TPPS could be a good PS candidate to kill the plant pathogen B. cinerea and be a safe option for grapevine (Vitis vinifera L.) explants.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

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Botrytis cinerea is a necrotic fungal plant pathogen responsible for the gray mold disease on more than 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents a strong resistance to a large spectrum of fungicides. Thus, new fighting strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea; upon light exposure. 1.5 µM TPPS was shown to completely inhibit mycelial growth. A concentration of 12.5 µM TPPS was tested on three genetic background clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds was able to increase thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, two-month-old grapevine leaves were infected with four-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the isolated leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results on fungus, plantlets and Botrytis-grapevine leaves, in contact with a very low concentration of TPPS, suggest a strong potential of photo-treatment against Botrytis mycelium for future agricultural practices in vineyard or other cultures.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

Self Cite

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“…2018) as opposed to what is observed with anionic porphyrins that can cause plant cell death (Leroy‐Lhez et al . 2019).…”

Section: Resultsmentioning

confidence: 99%

Photodynamic inactivation of the phytopathogenic bacterium Xanthomonas citri subsp. citri

Ndemueda

Pereira

Faustino

2020

Lett Appl Microbiol

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The present work intended to evaluate the applicability of photodynamic inactivation (PDI) of Xanthomonas citri subsp. citri with toluidine blue O (TBO), a commercial photosensitizer, as a strategy to control citrus canker. Assays were conducted with cell suspensions and biofilms, constructed either on polypropylene microtubes (in vitro assays) or on the surface of orange leaves (ex vivo assays), in the presence of TBO and under irradiation with artificial white light or natural sunlight. PDI assays using TBO alone caused a maximum 5·8 log10 reduction of X. citri viable cells in suspensions, and a much smaller inactivation (1·5 log10) in biofilms. However, concomitant use of KI potentiated the TBO photosensitization. Biofilms were inactivated down to the detection limit (>6 log10 reduction) with 5·0 µmol l−1 TBO + 10 mmol l−1 KI (in vitro) or 5·0 µmol l−1 TBO + 100 mmol l−1 KI (ex vivo) after artificial white light irradiation. Under natural sunlight, a reduction down to the detection limit of the Miles–Misra method was achieved with 50 µmol l−1 TBO and 100 mmol l−1 KI. PDI has potential to be applied in the control of citrus canker in field conditions although further studies are needed to show that there are no risks to plant physiology or fruit quality. Significance and Impact of the Study Xanthomonas citri subsp. citri is a major cause of disease in citrus orchards. Because of the low efficacy and high environmental toxicity of copper‐based treatments, there is growing interest on more sustainable phytosanitary approaches. Photodynamic inactivation (PDI) is being successfully used to control infectious agents and literature reports indicate that it is effective against some fungi and bacteria attacking fruit crops. The results of the present work open the perspective of using a low‐cost photosensitizer and sunlight, as energy source, to control of the causative agent of citrus canker.

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“…With the aim to develop APDT for agriculture applications, TPPS could be a good candidate because of its low toxicity for plants. Moreover, TPPS remains negatively charged in many chemical environments even under acidic pH and does not aggregate in solution, allowing it to easily permeate cells through cell walls and membranes 29,30 . Therefore, we hypothesized that TPPS could be a good PS candidate to kill the plant pathogen B. cinerea and has potential to be a safe option for grapevine (Vitis vinifera L.) explants.…”

mentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Ambrosini

Issawi

Sol

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Botrytis cinerea is a necrotic plant fungus that causes gray mold disease in over 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents strong resistance to many fungicides. Thus, new strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination with white light. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea using light. 1.5 µM TPPS completely inhibited mycelial growth. TPPS (12.5 µM) was tested on three grapevine clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds increased thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, 2-month-old grapevine leaves were infected with 4-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the detached leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results suggest a strong potential of photo-treatment against B. cinerea mycelium for future agricultural practices in vineyard or other cultures.

show abstract

Why are the anionic porphyrins so efficient to induce plant cell death? A structure-activity relationship study to solve the puzzle

Cited by 8 publications

References 59 publications

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Photodynamic inactivation of the phytopathogenic bacterium Xanthomonas citri subsp. citri

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

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