Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Ambrosini, Veronica; Issawi, Mohammad; Sol, Vincent; Riou, Catherine

doi:10.1038/s41598-020-74427-9

Cited by 17 publications

(17 citation statements)

References 54 publications

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“…They also were able to show in a trial with grapevine clones (Chardonnay, Merlot, Sauvignon), that 12.5 µM TPPS has no adverse effect on grapevine roots. Botrytis cinerea on grapevine vs − 1.5 µM TPPS was shown to completely inhibit mycelial growth [28]. Gonzales et al [29] used 25 and 50 µM Methylene blue and 30 min sunlight illumination to treat petals and leaves of sweet orange plants infected with conidia of Colletotricum abscissum and were able to obtain a 3 log 10 reduction-and complete eradication of conidia in some samples.…”

Section: Resultsmentioning

confidence: 99%

Photodynamic Inactivation of plant pathogens part II: fungi

Hamminger

Glueck

Fefer

et al. 2022

Photochem Photobiol Sci

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The constantly increasing demand for agricultural produce from organic and conventional farming calls for new, sustainable, and biocompatible solutions for crop protection. The overuse of fungicides leading to contamination of both produce and environment and the emergence of plant pathogenic fungi that are resistant to conventional treatments warrant the need for new methods to combat fungal infections in the field. We here deliver the follow-up study to our research on the Photodynamic Inactivation (PDI) of plant pathogenic bacteria (Glueck et al. in Photochem Photobiol Sci 18(7):1700–1708, 2019) by expanding the scope to fungal pathogens. Both fungal species employed in this study—Alternaria solani and Botrytis cinerea—cause substantial crop and economic losses. Sodium magnesium chlorophyllin (Chl, approved as food additive E140) in combination with Na2EDTA and the chlorin e6 derivative B17-0024 holding cationic moieties serve as eco-friendly photoactive compounds. Effectiveness of the antifungal PDI was measured by inhibition of growth of mycelial spheres (average diameter 2–3 mm) after incubation with the photosensitizer for 100 min and subsequent illumination using a LED array (395 nm, 106.6 J cm−2). One hundred micromolar Chl combined with 5 mM Na2EDTA was able to successfully photokill 94.1% of A. solani and 91.7% of B. cinerea samples. PDI based on B17-0024 can completely inactivate A. solani at 10 times lower concentration (10 µM); however, for B. cinerea, the concentration required for complete eradication was similar to that of Chl with Na2EDTA (100 µM). Using a plant compatibility assay based on Fragaria vesca, we further demonstrate that both photosensitizers neither affect host plant development nor cause significant leaf damage. The plants were sprayed with 300 µL of treatment solution used for PDI (one or three treatments on consecutive days) and plant growth was monitored for 21 days. Only minor leaf damage was observed in samples exposed to the chelators Na2EDTA and polyaspartic acid, but overall plant development was unaffected. In conclusion, our results suggest that sodium magnesium chlorophyllin in combination with EDTA and B17-0024 could serve as effective and safe photofungicides. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Photodynamic Inactivation of plant pathogens part II: fungi

Hamminger

Glueck

Fefer

et al. 2022

Photochem Photobiol Sci

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“…In the former category, we found that organoheterocyclic compounds, metabolites associated with nucleic acids, organic acids and carbohydrates, were all enriched in resistant individuals regardless of time. The metabolite superclass of organoheterocyclic compounds, including subclasses such as carbazole, porphyrin, and butanolide, are almost universally associated with anti-microbial or antioxidant activities [48][49][50]. Therefore, induction of these compounds by the onset of fungal colonisation within resistant individuals is likely to enhance defence against the pathogen.…”

Section: Melaleuca Disease Resistance Involves Mfs That Are Both Phen...mentioning

confidence: 99%

Both Constitutive and Infection-Responsive Secondary Metabolites Linked to Resistance against Austropuccinia psidii (Myrtle Rust) in Melaleuca quinquenervia

et al. 2022

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Austropuccinia psidii is a fungal plant pathogen that infects species within the Myrtaceae, causing the disease myrtle rust. Myrtle rust is causing declines in populations within natural and managed ecosystems and is expected to result in species extinctions. Despite this, variation in response to A. psidii exist within some species, from complete susceptibility to resistance that prevents or limits infection by the pathogen. Untargeted metabolomics using Ultra Performance Liquid Chromatography with Ion Mobility followed by analysis using MetaboAnalyst 3.0, was used to explore the chemical defence profiles of resistant, hypersensitive and susceptible phenotypes within Melaleuca quinquenervia during the early stages of A. psidii infection. We were able to identify three separate pools of secondary metabolites: (i) metabolites classified structurally as flavonoids that were naturally higher in the leaves of resistant individuals prior to infection, (ii) organoheterocyclic and carbohydrate-related metabolites that varied with the level of host resistance post-infection, and (iii) metabolites from the terpenoid pathways that were responsive to disease progression regardless of resistance phenotype suggesting that these play a minimal role in disease resistance during the early stages of colonization of this species. Based on the classes of these secondary metabolites, our results provide an improved understanding of key pathways that could be linked more generally to rust resistance with particular application within Melaleuca.

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“…This therapy was also used successfully against phytopathogenic fungi such as Lasiodiplodia theobromae (causes vine trunk disease), Botrytis cinerea (causes plant necrosis), and Colletotrichum sp. (causes anthracnose in various fruit trees) [ 26 , 27 , 28 ]. As already mentioned, spores are crucial to spreading fungal diseases, and fungal conidia are relevant targets for photosensitization [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

Gamelas

Sierra-García

Tomé

et al. 2023

IJMS

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Antimicrobial photodynamic therapy (aPDT) has been explored as an innovative therapeutic approach because it can be used to inactivate a variety of microbial forms (vegetative forms and spores) without causing significant damage to host tissues, and without the development of resistance to the photosensitization process. This study assesses the photodynamic antifungal/sporicidal activity of tetra- and octasubstituted phthalocyanine (Pc) dyes with ammonium groups. Tetra- and octasubstituted zinc(II) phthalocyanines (1 and 2) were prepared and tested as photosensitizers (PSs) on Fusarium oxysporum conidia. Photoinactivation (PDI) tests were conducted with photosensitizer (PS) concentrations of 20, 40, and 60 µM under white-light exposure at an irradiance of 135 mW·cm–2, applied during 30 and 60 min (light doses of 243 and 486 J·cm−2). High PDI efficiency corresponding to the inactivation process until the detection limit was observed for both PSs. The tetrasubstituted PS was the most effective, requiring the lowest concentration and the shortest irradiation time for the complete inactivation of conidia (40 µM, 30 min, 243 J·cm−2). Complete inactivation was also achieved with PS 2, but a longer irradiation time and a higher concentration (60 µM, 60 min, 486 J·cm−2) were necessary. Because of the low concentrations and moderate energy doses required to inactivate resistant biological forms such as fungal conidia, these phthalocyanines can be considered potent antifungal photodynamic drugs.

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Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Cited by 17 publications

References 54 publications

Photodynamic Inactivation of plant pathogens part II: fungi

Photodynamic Inactivation of plant pathogens part II: fungi

Both Constitutive and Infection-Responsive Secondary Metabolites Linked to Resistance against Austropuccinia psidii (Myrtle Rust) in Melaleuca quinquenervia

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

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