Whole transverse section and specific-tissue analysis of secondary metabolites in seven different grades of root of Paeonia lactiflora using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry

Wang, Qiuling; Liang, Zhitao; Peng, Yong; Hou, Jun; Wei, Sheng; Zhao, Zhuo; Wang, Wen Quan

doi:10.1016/j.jpba.2014.10.020

Cited by 23 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phenol paeonol is a commonly found constituent of the Paeonia genus and a constituent in P. veitchii. Its content in P. lactiflora is less clear with some study samples detecting extractable amounts of paeonol (Nizamutdinova et al, 2007;Chen et al, 2014) and other study samples where paeonol was undetectable (Chuang et al, 1996;Wang et al, 2014a). Paeonol's (C 9 H 10 O 3 ) similar chemical structure to aspirin (C 9 H 8 O 4 ) has seen significant research of its biological activity and therapeutic potential for diseases such as diabetes and atherosclerosis (Kim et al, 2004;Kim et al, 2009a;Bao et al, 2013;Fan et al, 2013;Liu et al, 2013;Liu et al, 2014a;Jin et al, 2016).…”

Section: Processing Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

A Pharmacological Review of Bioactive Constituents ofPaeonia lactifloraPallas andPaeonia veitchiiLynch

et al. 2016

View full text Add to dashboard Cite

The roots of two peony species, Paeonia lactiflora Pallas and Paeonia veitchii Lynch are routinely referred to as either chishao () or baishao (). This paper reviews the botanical origins and traditional medicinal usage of each species, as well as pharmacological like activity of their constituents. A search of herbal pharmacological encyclopaedia, PubChem and PubMed databases identified their known constituents. The biological data for these constituents were evaluated and classified according to pharmacological-like activity, with emphasis on compounds of greatest concentration and bioavailability. It was found that P. lactiflora and P. veitchii have some common compounds; however, their phytochemical bioavailability varies. Furthermore, a larger number of compounds have been identified in P. lactiflora. These have greater potential for antiinflammatory, antiviral, antibacterial and antioxidant therapeutic activity compared with P. veitchii. However, evidence indicates both species are similarly indicated for antiviral and glycaemic activity. Major compounds of each are classified as flavonoids, hydrolysable tannins (polyphenols) and monoterpene glycosides. The evidence suggests both species, when administered in entire botanical form, have an excellent safety profile; however, constituent toxicity risk evidence is limited, requiring further investigation. Although experiments show many compounds have biological activity, further investigation of their therapeutic potential is needed. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Processing Techniquesmentioning

confidence: 99%

“…A more recent study has indicated P. lactiflora roots less than 0.7 cm in diameter or larger than 1.7 cm have the greatest content of compounds, and cork of roots should remain. Such content of compounds is reported to be lower in the phloem and xylem of P. lactiflora (Wang et al ., ).…”

Section: Introductionmentioning

confidence: 97%

A Pharmacological Review of Bioactive Constituents ofPaeonia lactifloraPallas andPaeonia veitchiiLynch

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Thus the relationship between bioactive components and morphological features can be found by analyzing the distribution patterns of chemical components in different tissues. As a result, tissue- and cell-specific chemical profiling can link morphological features with secondary metabolites [ 10 , 11 , 12 ]. As such, both entire CMM sample and tissue-specific chemical profiling can be used to link morphological features and the quality of CMMs.…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Liang

et al. 2018

Molecules

View full text Add to dashboard Cite

In commercial herbal markets, Polygoni Multiflori Radix (PMR, the tuberous roots of Polygonum multiflorum Thunb.), a commonly-used Chinese medicinal material, is divided into different grades based on morphological features of size and weight. While more weight and larger size command a higher price, there is no scientific data confirming that the more expensive roots are in fact of better quality. To assess the inherent quality of various grades and of various tissues in PMR and to find reliable morphological indicators of quality, a method combining laser microdissection (LMD) and ultra-performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-QqQ-MS/MS) was applied. Twelve major chemical components were quantitatively determined in both whole material and different tissues of PMR. Determination of the whole material revealed that traditional commercial grades based on size and weight of PRM did not correspond to any significant differences in chemical content. Instead, tissue-specific analysis indicated that the morphological features could be linked with quality in a new way. That is, PMR with broader cork and phloem, as seen in a transverse section, were typically of better quality as these parts are where the bioactive components accumulate. The tissue-specific analysis of secondary metabolites creates a reliable morphological criterion for quality grading of PMR.

show abstract

“…This produced fragment ions at m/z 629, 509, and 491 by a series of losses of one H 2 O molecule, one benzoic acid molecule, and one H 2 O molecule, respectively. Compared with the literature [ 20 ], peak 8 was tentatively characterized as 6-O-galloyloxypaeoniflorin. Peak 25 gave the [M-H] − ions at m/z 599.1769 (C 30 H 32 O 13 ) and was 104 Da more than that of peak 3 .…”

Section: Resultsmentioning

confidence: 83%

Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

Shao

Zhao

Wang

et al. 2018

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.

show abstract

Whole transverse section and specific-tissue analysis of secondary metabolites in seven different grades of root of Paeonia lactiflora using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry

Cited by 23 publications

References 20 publications

A Pharmacological Review of Bioactive Constituents ofPaeonia lactifloraPallas andPaeonia veitchiiLynch

A Pharmacological Review of Bioactive Constituents ofPaeonia lactifloraPallas andPaeonia veitchiiLynch

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

Contact Info

Product

Resources

About