Whole organism responses and intersex severity in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part A

Bahamonde, Paulina; Fuzzen, Meghan; Bennett, Charles H.; Tetreault, Gerald R.; McMaster, Mark E.; Servos, Mark R.; Martyniuk, Christopher J.; Munkittrick, Kelly R.

doi:10.1016/j.aquatox.2014.11.023

Cited by 61 publications

(44 citation statements)

References 57 publications

(83 reference statements)

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“…While smaller relative gonad size has been found intermittently dependent upon season and year, impaired sex steroid production and the presence of intersex has been consistently found downstream of the Kitchener MWWTP (Bahamonde et al, 2014;Tanna et al, 2013;Tetreault et al, 2011). Additionally, male rainbow darter collected downstream of the Kitchener MWWTP have elevated levels of liver vitellogenin expression, a yolk protein precursor that is normally highly expressed in females, but not males (Bahamonde et al, 2014).…”

Section: Reproductive Healthmentioning

confidence: 94%

“…Previous studies have demonstrated feminization of male rainbow darter (Etheostoma caeruleum) caught near the outfalls of the larger MWWTPs in the Grand River watershed in Southern Ontario (Bahamonde et al, 2014;Tanna et al, 2013;Tetreault et al, 2011). Decreased testis size, a high incidence of intersex (>80%), decreased androgen production, and elevated vtg expression in the liver was found in male rainbow darter (Bahamonde et al, 2014;Tanna et al, 2013;Tetreault et al, 2011).…”

Section: Introductionmentioning

confidence: 90%

“…Decreased testis size, a high incidence of intersex (>80%), decreased androgen production, and elevated vtg expression in the liver was found in male rainbow darter (Bahamonde et al, 2014;Tanna et al, 2013;Tetreault et al, 2011). Despite this feminization, there is still a population of fish present, although at lower densities, downstream of the largest MWWTP (Kitchener) in the Grand River .…”

Section: Introductionmentioning

confidence: 91%

See 2 more Smart Citations

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

et al. 2015

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Section: Reproductive Healthmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 91%

See 1 more Smart Citation

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

et al. 2015

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“…Application of transcriptomics in aquatic ecotoxicology represents a powerful genome-based tool that allows one to obtain detailed insight into toxicological responses [20]. However, only a small subset of these studies have examined molecular responses in wild fish populations residing downstream of MWWTP discharges [5,[22][23][24][25]. The oligonucleotide microarray is a tool that can provide insights into transcriptome patterns, allowing one to examine thousands of genes in unison.…”

Section: Introductionmentioning

confidence: 99%

“…This species is highly abundant throughout the Grand River watershed, and it has a relatively small home range, moving only a few meters, mostly in the spring during spawning [26,27]. High incidence of intersex condition (testes-ova) [26,31], decreased production of androgens [25,26], and reduced reproductive performance [15] have been observed in rainbow darter downstream of the wastewater outfalls. Recent studies have shown that complex mixtures of pollutants released from the 2 major municipal wastewater outfalls in the central reaches of the Grand River (i.e., Kitchener and Waterloo) have resulted in endocrine disruption in rainbow darter.…”

Section: Introductionmentioning

confidence: 99%

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Marjan

Martyniuk

Fuzzen

et al. 2017

Enviro Toxic and Chemistry

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The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1223) remained comparable to that downstream of Waterloo (767-3867). Noteworthy was that year and the interaction of year and site explained variability in more than twice the number of transcripts than site alone, suggesting that year and the interaction of year and site had a greater effect on the transcriptome than site alone. Gene set enrichment analysis revealed a gradual reduction in the number of gene ontologies over time at exposure sites, which corresponded with lower contaminant load. Subnetwork enrichment analysis revealed that there were noticeable shifts in the cell pathways differently expressed in the liver preupgrade and postupgrade. The dominant pathways altered preupgrade were related to genetic modifications and cell division, whereas postupgrade they were associated with the immune system, reproduction, and biochemical responses. Molecular pathways were dynamic over time, and following the upgrades, there was little evidence that gene expression profiles in fish collected from high-impact sites postupgrade were more similar to those in fish collected from reference site. Environ Toxicol Chem 2017;36:2108-2122. © 2017 SETAC.

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Histopathologic Prevalence and Severity of Testicular Oocytes in Smallmouth Bass from Two Archival Collections

et al. 2020

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During recent decades, survey studies have documented the widespread presence of oocytes in the testes of male Smallmouth Bass Micropterus dolomieu collected from surface waters throughout the United States. There are few published reports of testicular oocytes (TO) in Smallmouth Bass before the 1990s, so it is difficult to know how long this has been occurring. Consequently, this study was conducted to evaluate the prevalence and severity of TO occurrence in whole fish specimens from two archival collections—the Smithsonian Institution's National Museum of Natural History in Suitland, Maryland, and Cornell University's Museum of Vertebrates in Ithaca, New York. Gonads were excised from 167 preserved male Smallmouth Bass that were originally collected between 1875 and 2004, and routine histologic sections were prepared and examined. The severity of TO was determined using a semiquantitative scoring system. Overall, 52.1% of male Smallmouth Bass were found to have TO. Affected fish had been collected in 11 of the 18 represented states, and TO were found in specimens harvested during decades as early as the 1880s and 1900s. Unfortunately, the small number of samples acquired at the earliest time periods precluded analyses of prevalence and severity trends over time. The results of this study demonstrated that the phenomenon of TO in male Smallmouth Bass is at least a century old and confirmed the widespread nature of this finding throughout the species’ historic range. Further research efforts should focus on determining the baseline prevalence of TO in laboratory‐reared male Smallmouth Bass that have not been exposed to endocrine active substances or the effects of experimental estrogen exposure on such fish.

show abstract

Whole organism responses and intersex severity in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part A

Cited by 61 publications

References 57 publications

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Histopathologic Prevalence and Severity of Testicular Oocytes in Smallmouth Bass from Two Archival Collections

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