Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models

Daryabari, Seyedeh Sima; Fendereski, Kiarad; Ghorbani, Fariba; Dehnavi, Mehrshad; Shafikhani, Yazdan; Omranipour, Ara; Davani, Sam Zeraatian-Nejad; Zolbin, Masoumeh Majidi; Tavangar, Seyed Mohammad; Kajbafzadeh, Abdol‐Mohammad

doi:10.1007/s10815-022-02492-2

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…Earlier this year, Daryabari et al published the first study that utilized perfusion decellularization of a whole-human uterus [46]. Histological evaluation of the decellularized tissue demonstrated the removal of cellular components while maintaining important ECM molecules using an SDS-based protocol.…”

Section: Progress In Human Uterus Bioengineering With Decellularized ...mentioning

confidence: 99%

“…Interestingly, the authors presented a protocol that used a relatively high detergent concentration and a long perfusion time compared to earlier published protocols on the sheep uterus. This indicates that protocols need to be developed and fine-tuned for every species and that decellularization protocols are not necessarily generic [17,28,29,46]. Further evaluation of the decellularized human uterus was conducted using computed tomography (CT) angiography and showed that the vascular conduit network was well preserved.…”

Section: Progress In Human Uterus Bioengineering With Decellularized ...mentioning

confidence: 99%

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Progress in Preclinical Research on Uterus Bioengineering That Utilizes Scaffolds Derived from Decellularized Uterine Tissue

Sehic

Brännström

Hellström

2022

Biomedical Materials & Devices

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During the last decade, uterus transplantation has evolved as the first treatment for absolute uterine factor infertility, caused by absence of a functional uterus. Current challenges in the area of uterus transplantation are organ shortage and side effects of immunosuppression. These hurdles may be solved with novel tissue engineering technologies to produce a uterus from stem cells. For example, the development of patient-specific grafts using a biomaterial together with the patient’s own cells might be utilized for a partial uterus repair therapy or a whole bioengineered uterus might be developed to replace an allogeneic graft in a uterus transplantation setting. During recent years, uterus bioengineering strategies with scaffolds based on decellularized tissue have been particularly assessed. Decellularization protocols were established for both small and large animal models, including the human uterus. Promising in vivo results using such scaffolds to repair a partially injured uterus showed restoration of fertility in rodent models. Scaffold generation protocols and recellularization methodologies including various cell sources are currently being optimized and translated to more clinically relevant injury models in large animals. This review provides a summary of the progress made to date, based on use of decellularized uterine tissue for uterus repair.

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Section: Progress In Human Uterus Bioengineering With Decellularized ...mentioning

confidence: 99%

Section: Progress In Human Uterus Bioengineering With Decellularized ...mentioning

confidence: 99%

Progress in Preclinical Research on Uterus Bioengineering That Utilizes Scaffolds Derived from Decellularized Uterine Tissue

Sehic

Brännström

Hellström

2022

Biomedical Materials & Devices

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“…16 For these reasons, whole uterus ECM based scaffolds were created through decellularization for many species, including the mouse, 17 rat, [18][19][20][21] rabbit, 22 pig, 23 sheep, [24][25][26] and human. 27 Multiple independent labs showed fertility restoration in the rat model using small-sized uterine grafts, but these principles have not yet been evaluated in large animal models with a uterus of similar size as the human and thereby consisting of clinically relevant sized grafts.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of a bioengineered tissue patch promotes uterine repair in the sheep

Sehic,

de Miguel Gómez,

Rabe

et al. 2024

Biomater. Sci.

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“…Multiple strategies and processes have been adopted for this goal and to respond to the challenges described above, each of which separately plays a vital role in the endothelialization process ( Seiffert et al, 2021 ; Daryabari et al, 2022 ; Haeublein et al, 2022 ; Kim et al, 2022 ). Decellularization and its protocols are among the first influential factors in the endothelialization of acellular structures.…”

Section: Introductionmentioning

confidence: 99%

“…They exert their effect due to the degree of success in removing cellular materials, as well as their impact on the acellular scaffold and ECM microstructure alterations. So that the more intact the obtained acellular structure is, the better the endothelialization outcome will be obtained ( Daryabari et al, 2022 ). This point is also valid in recellularization conditions.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Mazloomnejad

Babajani

Kasravi

et al. 2023

Front. Bioeng. Biotechnol.

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Decellularization of tissues and organs has recently become a promising approach in tissue engineering and regenerative medicine to circumvent the challenges of organ donation and complications of transplantations. However, one main obstacle to reaching this goal is acellular vasculature angiogenesis and endothelialization. Achieving an intact and functional vascular structure as a vital pathway for supplying oxygen and nutrients remains the decisive challenge in the decellularization/re-endothelialization procedure. In order to better understand and overcome this issue, complete and appropriate knowledge of endothelialization and its determining variables is required. Decellularization methods and their effectiveness, biological and mechanical characteristics of acellular scaffolds, artificial and biological bioreactors, and their possible applications, extracellular matrix surface modification, and different types of utilized cells are factors affecting endothelialization consequences. This review focuses on the characteristics of endothelialization and how to optimize them, as well as discussing recent developments in the process of re-endothelialization.

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Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models

Cited by 11 publications

References 29 publications

Progress in Preclinical Research on Uterus Bioengineering That Utilizes Scaffolds Derived from Decellularized Uterine Tissue

Progress in Preclinical Research on Uterus Bioengineering That Utilizes Scaffolds Derived from Decellularized Uterine Tissue

Transplantation of a bioengineered tissue patch promotes uterine repair in the sheep

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

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