Whole genome strategies and bioremediation insight into dehalogenase-producing bacteria

Oyewusi, Habeebat Adekilekun; Wahab, Roswanira Abdul; Huyop, Fahrul

doi:10.1007/s11033-021-06239-7

Cited by 15 publications

(9 citation statements)

References 95 publications

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“…Reductive dehalogenation (anaerobic) processes are carried out by genera such as Dehalobacter, Dehalobacterium , and Dehalococcoides [ 48 , 49 , 50 , 51 , 52 , 53 , 54 ]. These were found in all studied metagenomes, but in small relative abundance (<0.01%); however, two other reductive dehalogenases were found, tetrachloroethene reductive dehalogenase and tetrachlorohydroquinone reductive dehalogenase, the latter only identified in the metagenome that came from the wastewater treatment plant.…”

Section: Discussionmentioning

confidence: 99%

Potential of Microbial Communities to Perform Dehalogenation Processes in Natural and Anthropogenically Modified Environments—A Metagenomic Study

2023

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Halogenated organic compounds (HOCs) pose a serious problem for the environment. Many are highly toxic and accumulate both in soil and in organisms. Their biological transformation takes place by dehalogenation, in which the halogen substituents are detached from the carbon in the organic compound by enzymes produced by microorganisms. This increases the compounds’ water solubility and bioavailability, reduces toxicity, and allows the resulting compound to become more susceptible to biodegradation. The microbial halogen cycle in soil is an important part of global dehalogenation processes. The aim of the study was to examine the potential of microbial communities inhabiting natural and anthropogenically modified environments to carry out the dehalogenation process. The potential of microorganisms was assessed by analyzing the metagenomes from a natural environment (forest soils) and from environments subjected to anthropopression (agricultural soil and sludge from wastewater treatment plants). Thirteen genes encoding enzymes with dehalogenase activity were identified in the metagenomes of both environments, among which, 2-haloacid dehalogenase and catechol 2,3-dioxygenase were the most abundant genes. Comparative analysis, based on comparing taxonomy, identified genes, total halogens content and content of DDT derivatives, demonstrated the ability of microorganisms to transform HOCs in both environments, indicating the presence of these compounds in the environment for a long period of time and the adaptive need to develop mechanisms for their detoxification. Metagenome analyses and comparative analyses indicate the genetic potential of microorganisms of both environments to carry out dehalogenation processes, including dehalogenation of anthropogenic HOCs.

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Section: Discussionmentioning

confidence: 99%

Potential of Microbial Communities to Perform Dehalogenation Processes in Natural and Anthropogenically Modified Environments—A Metagenomic Study

2023

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“…More recently, dehalogenases have drawn attention for environmental remediation of organohalides, which are notoriously known for their persistence in the environment [ 78 , 79 , 80 ]. They include chlorinated [ 81 ], brominated [ 82 ], and fluorinated [ 83 ] species, such as pesticides and waste products from various industrial activities.…”

Section: Hydrolase Applicationsmentioning

confidence: 99%

Short Peptides for Hydrolase Supramolecular Mimicry and Their Potential Applications

Alletto,

Garcia,

Marchesan

2023

Gels

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Hydrolases are enzymes that have found numerous applications in various industrial sectors spanning from pharmaceuticals to foodstuff and beverages, consumers’ products such as detergents and personal care, textiles, and even for biodiesel production and environmental bioremediation. Self-assembling and gelling short peptides have been designed for their mimicry so that their supramolecular organization leads to the creation of hydrophobic pockets for catalysis to occur. Catalytic gels of this kind can also find numerous industrial applications to address important global challenges of our time. This concise review focuses on the last 5 years of progress in this fast-paced, popular field of research with an eye towards the future.

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“…The 2-haloacid dehalogenases can detoxify halogenated pollutants by hydrolysis without the addition of other reductive agents; for this reason, their potential application in bioremediation is particularly attractive (Behbahani et al, 2018;Oyewusi et al, 2020bOyewusi et al, , 2021bZakary et al, 2021). The 2-haloacid dehalogenases are also highly stereoselective, and they may therefore be valuable in fine chemistry synthesis applications (Chen and Ribeiro de Souza, 2019;Adamu et al, 2020;.…”

Section: Applicationmentioning

confidence: 99%

“…The accumulation of these pollutants causes serious environmental problems and threats to human and other organisms' health. The 2-haloacid dehalogenase can catalyze the dehalogenation of 2-chloropropionic acids and 2,2-dichloropropionic acids to form non-toxic hydroxyl acids, which is a very promising potential tool for environmental bioremediation (Oyewusi et al, 2021b;Zakary et al, 2021). Dioxin compounds are carcinogenic byproducts originating from natural and anthropogenic sources such as herbicides, pesticides, and combustion processes; high levels of dioxin-contamination have been reported in food, soils, and blood samples of local residents in Southern Vietnam (Nguyen et al, 2021).…”

Section: Environmental Bioremediationmentioning

confidence: 99%

“…As the main decomposers in nature, microorganisms convert complex organic compounds into simple compounds, thus maintaining the cycle of elements that are vital to life (Hellal et al, 2021;Kajla et al, 2021;Yu et al, 2021). Microorganisms growing in environments polluted by organic halogenated compounds have the potential to transform these compounds owing to the presence of enzymes that catalyze dehalogenation in their cells, called dehalogenases (Atashgahi et al, 2018;Oyewusi et al, 2020bOyewusi et al, , 2021b. Among them, 2-haloacid dehalogenases are a family of critical enzymes that hydrolytically catalyze the dehalogenation of 2-haloacids to form corresponding 2hydroxy acids (Kurihara and Esaki, 2008;Adamu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Mini Review: Advances in 2-Haloacid Dehalogenases

et al. 2021

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The 2-haloacid dehalogenases (EC 3.8.1.X) are industrially important enzymes that catalyze the cleavage of carbon–halogen bonds in 2-haloalkanoic acids, releasing halogen ions and producing corresponding 2-hydroxyl acids. These enzymes are of particular interest in environmental remediation and environmentally friendly synthesis of optically pure chiral compounds due to their ability to degrade a wide range of halogenated compounds with astonishing efficiency for enantiomer resolution. The 2-haloacid dehalogenases have been extensively studied with regard to their biochemical characterization, protein crystal structures, and catalytic mechanisms. This paper comprehensively reviews the source of isolation, classification, protein structures, reaction mechanisms, biochemical properties, and application of 2-haloacid dehalogenases; current trends and avenues for further development have also been included.

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Whole genome strategies and bioremediation insight into dehalogenase-producing bacteria

Cited by 15 publications

References 95 publications

Potential of Microbial Communities to Perform Dehalogenation Processes in Natural and Anthropogenically Modified Environments—A Metagenomic Study

Potential of Microbial Communities to Perform Dehalogenation Processes in Natural and Anthropogenically Modified Environments—A Metagenomic Study

Short Peptides for Hydrolase Supramolecular Mimicry and Their Potential Applications

Mini Review: Advances in 2-Haloacid Dehalogenases

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