Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

Smirnova, N. I.; Krasnov, Yaroslav M.; Agafonova, E. Yu.; Shchelkanova, E. Yu.; Al’khova, Zhanna V.; Кутырев, Владимир

doi:10.1128/genomea.01640-16

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…The wave-3 clade with ctxB7 allele has emerged from Kolkata, India in 2006 (29) and continues to spread globally, as seen in the Haiti, Yemen and other recent cholera epidemics. V. cholerae isolated from a patient during an outbreak of cholera in Mariupol, Ukraine, in 2011 also contained the ctxB7 (30). Almost all the V. cholerae isolates belonging to wave-3 contain ICE of the SXT/R391 family, mostly the ICE Vch Ind5.…”

Section: Cholera Waves Triggered By El Tor Vibriosmentioning

confidence: 99%

Revisiting the Global Epidemiology of Cholera in Conjunction With the Genomics of Vibrio cholerae

Ramamurthy

Mutreja

Weill

et al. 2019

Front. Public Health

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Toxigenic Vibrio cholerae is responsible for 1.4 to 4.3 million cases with about 21,000–143,000 deaths per year. Dominance of O1 and O139 serogroups, classical and El tor biotypes, alterations in CTX phages and the pathogenicity Islands are some of the major features of V. cholerae isolates that are responsible for cholera epidemics. Whole-genome sequencing (WGS) based analyses of single-nucleotide polymorphisms (SNPs) and other infrequent genetic variants provide a robust phylogenetic framework. Recent studies on the global transmission of pandemic V. cholerae O1 strains have shown the existence of eight different phyletic lineages. In these, the classical and El Tor biotype strains were separated as two distinctly evolved lineages. The frequency of SNP accumulation and the temporal and geographical distribution supports the perception that the seventh cholera pandemic (7CP) has spread from the Bay of Bengal region in three independent but overlapping waves. The 2010 Haitian outbreak shared a common ancestor with South-Asian wave-3 strains. In West Africa and East/Southern Africa, cholera epidemics are caused by single expanded lineage, which has been introduced several times since 1970. The Latin American epidemics that occurred in 1991 and 2010 were the result of introductions of two 7CP sublineages. Sublineages representing wave-3 have caused huge outbreaks in Haiti and Yemen. The Ogawa-Inaba serotype switchover in several cholera epidemics are believed to be due to the involvement of certain selection mechanism(s) rather than due to random events. V. cholerae O139 serogroup is phylogenetically related to the 7CP El Tor, and almost all these isolates belonged to the multilocus sequence type-69. Additional phenotypic and genotypic information have been generated to understand the pathogenicity of classical and El Tor vibrios. Presence of integrative conjugative elements (ICE) with antibiotic resistance gene cassettes, clustered regularly interspaced short palindromic repeats-associated protein system and ctxAB promoter based ToxRS expression of cholera toxin (CT) separates classical and El Tor biotypes. With the availability of WGS information, several important applications including, molecular typing, antimicrobial resistance, new diagnostics, and vaccination strategies could be generated.

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Section: Cholera Waves Triggered By El Tor Vibriosmentioning

confidence: 99%

Revisiting the Global Epidemiology of Cholera in Conjunction With the Genomics of Vibrio cholerae

Ramamurthy

Mutreja

Weill

et al. 2019

Front. Public Health

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“…Our strain belonged to Wave 3, isolates of which are known to harbour the CT-genotype 7 4 . The clade containing ctxB7 allele, to which our strain belongs, emerged in Kolkata in 2006 27 and has since been reported to have disseminated widely 28 . The tcpA gene codes for the major structural protein of the toxin-co-regulated pilus, which is the second major virulence factor of V. cholerae 20 .…”

Section: Discussionmentioning

confidence: 94%

Outbreak of cholera in a remote village in western India

Kumar

Barve²,

Gopalkrishna

et al. 2022

Indian J Med Res

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“…The number of cases was moderate, from 58 to 226 (refs. 4 , 24 , 25 ). Interestingly, EUR7 was not involved in the contemporary outbreaks in Ukraine and Moldova (see above), even though the first cases in 1995 were recorded in two Romanian towns on the border with Ukraine 20 .…”

Section: Resultsmentioning

confidence: 99%

The seventh pandemic of cholera in Europe revisited by microbial genomics

Oprea¹,

Njamkepo

Cristea³

et al. 2020

Nat Commun

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In 1970, the seventh pandemic of cholera (7 P) reached both Africa and Europe. Between 1970 and 2011, several European countries reported cholera outbreaks of a few to more than 2,000 cases. We report here a whole-genome analysis of 1,324 7 P V. cholerae El Tor (7 PET) isolates, including 172 from autochthonous sporadic or outbreak cholera cases occurring between 1970 and 2011 in Europe, providing insight into the spatial and temporal spread of this pathogen across Europe. In this work, we show that the 7 PET lineage was introduced at least eight times into two main regions: Eastern and Southern Europe. Greater recurrence of the disease was observed in Eastern Europe, where it persisted until 2011. It was introduced into this region from Southern Asia, often circulating regionally in the countries bordering the Black Sea, and in the Middle East before reaching Eastern Africa on several occasions. In Southern Europe, the disease was mostly seen in individual countries during the 1970s and was imported from North and West Africa, except in 1994, when cholera was imported into Albania and Italy from the Black Sea region. These results shed light on the geographic course of cholera during the seventh pandemic and highlight the role of humans in its global dissemination.

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Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

Cited by 6 publications

References 6 publications

Revisiting the Global Epidemiology of Cholera in Conjunction With the Genomics of Vibrio cholerae

Revisiting the Global Epidemiology of Cholera in Conjunction With the Genomics of Vibrio cholerae

Outbreak of cholera in a remote village in western India

The seventh pandemic of cholera in Europe revisited by microbial genomics

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