Whole-genome sequencing of Staphylococcus epidermidis bloodstream isolates from a prospective clinical trial reveals that complicated bacteraemia is caused by a limited number of closely related sequence types

Shelburne, Samuel A.; Dib, Rita Wilson; Endres, Bradley T.; Reitzel, Ruth; Li, X.; Kalia, Awdhesh; Sahasrabhojane, Pranoti; Chaftari, Anne-Marie; Hachem, Ray; Vargas-Cruz, Nylev; Jiang, Ying; Garey, Kevin W.; Fowler, Vance G.; Holland, David; Gu, Joan; Miller, William R.; Sakurai, Aki; Arias, César A.; Aitken, Samuel L; Greenberg, David E.; Kim, J.; Flores, Armida; Raad, Issam

doi:10.1016/j.cmi.2019.10.008

Cited by 11 publications

(7 citation statements)

References 27 publications

(54 reference statements)

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“…The shortening of the C-terminus of BT3147 was found to promote biofilm formation in B. thetaiotaomicron . This widespread but highly variable ability to form biofilms is observed in many aerobic bacteria, including Escherichia coli , 33 Staphylococcus epidermidis , 34 and Bacteroides fragilis , which also show a variable biofilm formation capacity caused by sigma factor RpoQ. 35 Biofilm formation by pathogenic bacteria is a major cause of chronic and recurring infections.…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

Feng

et al. 2020

ACS Omega

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This study is a bioinformatics assay on the microbial genome of Bacteroides thetaiotaomicron . The study focuses on the problem of quorum sensing as a result of adverse factors such as chemotherapy and antibiotic therapy. In patients with severe intestinal diseases, two strains of microorganisms were identified that were distinguished as new. Strains were investigated by conducting genome sequencing. The current concepts concerned with the quorum sensing system regulation by stationary-phase sigma factor and their coregulation of target genes in B. thetaiotaomicron were considered. The study suggested using bioinformatics data for the diagnosis of gastrointestinal disorders. In the course of the study, 402 genes having a greater than twofold change were identified with the 95% confidence level. The shortest and longest coding genes were predicted; the noncoding genes were detected. Biological pathways (KEGG pathways) were classified into the following categories: cellular processes, environmental information processing, genetic information processing, human disease, metabolism, and organismic systems. Among notable changes in the biofilm population observed in parallel to the planktonic B. thetaiotaomicron was the expression of genes in the polysaccharide utilization loci that were involved in the synthesis of O-glycans.

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Section: Discussionmentioning

confidence: 99%

Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

Feng

et al. 2020

ACS Omega

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“…Aiming to find a genotype-phenotype correlation, Shelburne and colleagues showed with whole-genome sequencing that certain sequence types of S . epidermidis predominate among patients with complicated BSI [ 59 ]. Sánchez and colleagues sought to find a genetic marker to distinguish between commensal and infecting strains of S. epidermidis in prosthetic joint infections [ 60 ].…”

Section: The Diagnostic Complexities—how To Distinguish Between Infection and Contaminationmentioning

confidence: 99%

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

et al. 2021

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Coagulase-negative staphylococci (CoNS) are among the most frequently recovered bacteria in routine clinical care. Their incidence has steadily increased over the past decades in parallel to the advancement in medicine, especially in regard to the utilization of foreign body devices. Many new species have been described within the past years, while clinical information to most of those species is still sparse. In addition, interspecies differences that render some species more virulent than others have to be taken into account. The distinct populations in which CoNS infections play a prominent role are preterm neonates, patients with implanted medical devices, immunodeficient patients, and those with other relevant comorbidities. Due to the property of CoNS to colonize the human skin, contamination of blood cultures or other samples occurs frequently. Hence, the main diagnostic hurdle is to correctly identify the cases in which CoNS are causative agents rather than contaminants. However, neither phenotypic nor genetic tools have been able to provide a satisfying solution to this problem. Another dilemma of CoNS in clinical practice pertains to their extensive antimicrobial resistance profile, especially in healthcare settings. Therefore, true infections caused by CoNS most often necessitate the use of second-line antimicrobial drugs.

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“…In hospital environments, two sequence types (STs), ST2 and ST23, account for most adult clinical disease (i.e., bacteremia, CVC infection, septic arthritis) [ 147 ]. Furthermore, having an ST2 or ST5 S. epidermidis bacteraemia has been found to be an independent predictor of complicated bloodstream infection [ 148 ]. Neonates in the NICU are most frequently colonized with S. epidermidis strains belonging to ST2, ST5, ST59, and ST81, and among LOS-causing isolates, ST2 and ST5 are most commonly identified [ 144 ].…”

Section: Genetic Determinants Of Virulence In Commensal and Invasive S Epidermidismentioning

confidence: 99%

Look Who’s Talking: Host and Pathogen Drivers of Staphylococcus epidermidis Virulence in Neonatal Sepsis

Joubert

Otto

Strunk

et al. 2022

IJMS

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Preterm infants are at increased risk for invasive neonatal bacterial infections. S. epidermidis, a ubiquitous skin commensal, is a major cause of late-onset neonatal sepsis, particularly in high-resource settings. The vulnerability of preterm infants to serious bacterial infections is commonly attributed to their distinct and developing immune system. While developmentally immature immune defences play a large role in facilitating bacterial invasion, this fails to explain why only a subset of infants develop infections with low-virulence organisms when exposed to similar risk factors in the neonatal ICU. Experimental research has explored potential virulence mechanisms contributing to the pathogenic shift of commensal S. epidermidis strains. Furthermore, comparative genomics studies have yielded insights into the emergence and spread of nosocomial S. epidermidis strains, and their genetic and functional characteristics implicated in invasive disease in neonates. These studies have highlighted the multifactorial nature of S. epidermidis traits relating to pathogenicity and commensalism. In this review, we discuss the known host and pathogen drivers of S. epidermidis virulence in neonatal sepsis and provide future perspectives to close the gap in our understanding of S. epidermidis as a cause of neonatal morbidity and mortality.

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Whole-genome sequencing of Staphylococcus epidermidis bloodstream isolates from a prospective clinical trial reveals that complicated bacteraemia is caused by a limited number of closely related sequence types

Abstract: Conclusion: S. epidermidis bacteraemia was caused by a genetically heterogeneous group of organisms, but only a limited number of STsdparticularly multidrug-resistant ST2 and ST5 strainsdcaused complicated infections.

Cited by 11 publications

References 27 publications

Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

Look Who’s Talking: Host and Pathogen Drivers of Staphylococcus epidermidis Virulence in Neonatal Sepsis

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