Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

Abstract

This study is a bioinformatics assay on the microbial genome of Bacteroides thetaiotaomicron . The study focuses on the problem of quorum sensing as a result of adverse factors such as chemotherapy and antibiotic therapy. In patients with severe intestinal diseases, two strains of microorganisms were identified that were distinguished as new. Strains were investigated by conducting genome sequencing. The current concepts concerned with the quorum sensing system regulation by stationary-phase sigma factor and their coregulation of target genes in B. thetaiotaomicron were considered. The study suggested using bioinformatics data for the diagnosis of gastrointestinal disorders. In the course of the study, 402 genes having a greater than twofold change were identified with the 95% confidence level. The shortest and longest coding genes were predicted; the noncoding genes were detected. Biological pathways (KEGG pathways) were classified into the following categories: cellular processes, environmental information processing, genetic information processing, human disease, metabolism, and organismic systems. Among notable changes in the biofilm population observed in parallel to the planktonic B. thetaiotaomicron was the expression of genes in the polysaccharide utilization loci that were involved in the synthesis of O-glycans.

Plant-Derived Quorum Sensing Inhibitors (Quercetin, Vanillin and Umbelliferon) Modulate Cecal Microbiome, Reduces Inflammation and Affect Production Efficiency in Broiler Chickens

“…Compared to the control group, which showed an approximately equal abundance of these phyla, the Bc : Bd calculation gave increased values of this ratio in all experimental groups, with the most expressed >10 with the VN + UF supplementation. In this regard, a recent report on quorum sensing in Bacteroidota phyla members [ 38 ] should be referred to, which may explain the QSI’s impact on the chicken’s gut microbiome. Another explanation could be an inhibitory effect of similar plant-derived molecules on Bacteroides lactate dehydrogenase [ 39 ] or a disruption of the starch utilization system, depriving these bacteria of their primary metabolic energy source [ 40 ].…”