Whole-Genome Sequencing of Mycoplasma mycoides subsp. mycoides Italian Strain 57/13, the Causative Agent of Contagious Bovine Pleuropneumonia

Orsini, Massimiliano; Krasteva, Ivanka; Marcacci, Maurilia; Ancora, Massimo; Ciammaruconi, Andrea; Gentile, Bernardina; Lista, Florigio; Pini, A.; Scacchia, Massimo; Sacchini, Flavio; Cammà, Cesare

doi:10.1128/genomea.00197-15

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…77 The genome of the 57/ 13 strain consisted of a single chromosome of 1 192 498 bp, and 1077 protein-encoding genes were identified of which 332 encode for hypothetical proteins conserved among other Mmm isolates. 77…”

Section: The Causative Agentmentioning

confidence: 99%

“…30,55 Several Mmm strains have been fully sequenced, showing very low diversity among genomes. 30,35,77,114,116 As an example, the genome of the Italian strain 57/13 showed an overall identity of 99% as compared with the type strain PG1 T . 77 The genome of the 57/ 13 strain consisted of a single chromosome of 1 192 498 bp, and 1077 protein-encoding genes were identified of which 332 encode for hypothetical proteins conserved among other Mmm isolates.…”

Section: The Causative Agentmentioning

confidence: 99%

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Contagious Bovine Pleuropneumonia: A Comprehensive Overview

et al. 2020

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Contagious bovine pleuropneumonia (CBPP) is a respiratory disease of cattle that is listed as notifiable by the World Organization for Animal Health. It is endemic in sub-Saharan Africa and causes important productivity losses due to the high mortality and morbidity rates. CBPP is caused by Mycoplasma mycoides subsp. mycoides ( Mmm) and is characterized by severe fibrinous bronchopneumonia and pleural effusion during the acute to subacute stages and by pulmonary sequestra in chronic cases. Additional lesions can be detected in the kidneys and in the carpal and tarsal joints of calves. Mmm infection occurs through the inhalation of infected aerosol droplets. After the colonization of bronchioles and alveoli, Mmm invades blood and lymphatic vessels and causes vasculitis. Moreover, Mmm can be occasionally demonstrated in blood and in a variety of other tissues. In the lung, Mmm antigen is commonly detected on bronchiolar and alveolar epithelial cells, in lung phagocytic cells, within the wall of blood and lymphatic vessels, inside necrotic areas, and within tertiary lymphoid follicles. Mmm antigen can also be present in the cytoplasm of macrophages within lymph node sinuses, in the germinal center of lymphoid follicles, in glomerular endothelial cells, and in renal tubules. A complete pathological examination is of great value for a rapid presumptive diagnosis, but laboratory investigations are mandatory for definitive diagnosis. The purpose of this review is to describe the main features of CBPP including the causative agent, history, geographic distribution, epidemiology, clinical course, diagnosis, and control. A special focus is placed on gross and microscopic lesions in order to familiarize veterinarians with the pathology and pathogenesis of CBPP.

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Section: The Causative Agentmentioning

confidence: 99%

Section: The Causative Agentmentioning

confidence: 99%

Contagious Bovine Pleuropneumonia: A Comprehensive Overview

et al. 2020

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Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations

Teodoro

Marruchella

Mosca

et al. 2018

Veterinary Immunology and Immunopathology

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Reactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indirectly measured using a luminol-based chemiluminescence assay. Our results confirm that Mmm can produce ROS via the metabolism of glycerol, significant differences existing between African and European strains. Mmm was capable of adhering to the external surface of neutrophils. Interestingly, Mmm enhanced the respiratory burst of bovine neutrophils. This activity was particularly pronounced with the African field strain and in presence of glycerol. Taken together, our data argue in favour of a major role for neutrophils as the main source of ROS in contagious bovine pleuropneumonia.

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High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237

Fischer¹,

Santana-Cruz²,

Hegerman³

et al. 2015

Stand in Genomic Sci

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Members of the Mycoplasma mycoides cluster’ represent important livestock pathogens worldwide. Mycoplasma mycoides subsp. mycoides is the etiologic agent of contagious bovine pleuropneumonia (CBPP), which is still endemic in many parts of Africa. We report the genome sequences and annotation of two frequently used challenge strains of Mycoplasma mycoides subsp. mycoides, Afadé and B237. The information provided will enable downstream ‘omics’ applications such as proteomics, transcriptomics and reverse vaccinology approaches. Despite the absence of Mycoplasma pneumoniae like cyto-adhesion encoding genes, the two strains showed the presence of protrusions. This phenotype is likely encoded by another set of genes.

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Whole-Genome Sequencing of Mycoplasma mycoides subsp. mycoides Italian Strain 57/13, the Causative Agent of Contagious Bovine Pleuropneumonia

Cited by 4 publications

References 9 publications

Contagious Bovine Pleuropneumonia: A Comprehensive Overview

Contagious Bovine Pleuropneumonia: A Comprehensive Overview

Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations

High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237

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