High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237

Fischer, Anne; Santana-Cruz, Ivette; Hegerman, Jan; Gourlé, Hadrien; Schieck, Elise; Lambert, Mathieu; Nadendla, Suvarna; Wesonga, Hezron; Miller, Robert W.; Vashee, Sanjay; Weber, Johann; Meens, Jochen; Frey, Joachim; Jores, Joerg

doi:10.1186/s40793-015-0067-0

Cited by 18 publications

(16 citation statements)

References 42 publications

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“…We used BLASTP and TBLASTN ( E < 10 − 5 ; low-complexity regions filtered out) to search for homologs of M. pneumoniae cytadhesins P1, P30, P65, P40 and P90 —proteins involved in adhesion— and cytadhesin accessory proteins Hmw1, Hmw2 and Hmw3 in all available Mycoplasma genomic data (nr database). We only found homologs in species closely related to M. pneumoniae ( Mycoplasma genitalium , Mycoplasma gallisepticum , Mycoplasma pirum , Mycoplasma alvi , Mycoplasma imitans , and M. testudinis ), as previously noted [ 38 , 39 ]. Searches against the M. testudineum BH29 T proteome detected no hits, and none of the 788 predicted M. testudineum proteins contained any of the Pfam domains present in the M. pneumoniae cytadhesins and accessory proteins (domains “CytadhesinP1”, “Adhesin_P1”, “Cytadhesin_P30”, “MgpC” and “EAGR_box”).…”

Section: Insights From the Genome Sequencesupporting

confidence: 78%

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

Weitzman

Tillett

Sandmeier

et al. 2018

Stand in Genomic Sci

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Mycoplasma testudineum is one of the pathogens that can cause upper respiratory tract disease in desert tortoises, Gopherus agassizii. We sequenced the genome of M. testudineum BH29T (ATCC 700618T = MCCM 03231T), isolated from the upper respiratory tract of a Mojave desert tortoise with upper respiratory tract disease. The sequenced draft genome, organized in 25 scaffolds, has a length of 960,895 bp and a G + C content of 27.54%. A total of 788 protein-coding sequences, six pseudogenes and 35 RNA genes were identified. The potential presence of cytadhesin-encoding genes is investigated. This genome will enable comparative genomic studies to help understand the molecular bases of the pathogenicity of this and other Mycoplasma species.

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Section: Insights From the Genome Sequencesupporting

confidence: 78%

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

Weitzman

Tillett

Sandmeier

et al. 2018

Stand in Genomic Sci

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“…These sequences were compared to the African field isolates Mycoplasma mycoides subsp. mycoides Afadé and B237 (22), and when discrepancies were detected, sequences originating from the African isolates were chosen for design of the fulllength recombinants. N-terminal signal peptides from lipoproteins, i.e., cleavage sites for signal peptidase II, were identified using the LipoP v 1.0 server (23) and were removed prior to gene synthesis.…”

Section: Methodsmentioning

confidence: 99%

Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

et al. 2016

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e Contagious bovine pleuropneumonia (CBPP) is a severe respiratory disease that is widespread in sub-Saharan Africa. It is caused by Mycoplasma mycoides subsp. mycoides, a bacterium belonging to the Mycoplasma mycoides cluster. In the absence of an efficient CBPP vaccine, improved and easy-to-use diagnostic assays for recurrent testing combined with isolation and treatment of positive animals represent an option for CBPP control in Africa. Here we describe the comprehensive screening of 17 immunogenic Mycoplasma mycoides subsp. mycoides proteins using well-characterized bovine sera for the development of a novel cocktail enzyme-linked immunosorbent assay (ELISA) for laboratory use. Two recombinant Mycoplasma immunogens, MSC_0136 and MSC_0636, were used to set up a standardized cocktail ELISA protocol. According to the results from more than 100 serum samples tested, the sensitivity and specificity of the novel cocktail ELISA were 85.6% and 96.4%, respectively, with an overall diagnostic accuracy comparable to that of the Office International des Epizooties (OIE)-prescribed serological assays. In addition, we provide a proof of principle for a field-applicable, easy-to-use commercially produced prototype lateral-flow test for rapid (<30-min) diagnosis of CBPP.

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“…Total DNA of the strains GM12, GM12::YCpMmyc1.1 and GM12::YCpMmyc1.1- Δ68 was isolated as described before (39). DNA was sheared using sonication and subjected to Illumina sequencing using a MiSeq machine by University of California Santa Cruz, CA (USA).…”

Section: Methodsmentioning

confidence: 99%

Removal of a subset of non-essential genes fully attenuates a highly virulentMycoplasmastrain

Jores

Stolzmann³

et al. 2019

Preprint

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IMPORTANCE 43Members of the Mycoplasma mycoides cluster cause important animal plaques in Africa and 44 Asia, which impact animal welfare, provision of food and the life of thousands of small-scale 45 farmers. We applied synthetic biology tools to Mycoplasma mycoides in order to design and 46 create a fully attenuated Mycoplasma strain that was subsequently confirmed in vivo using a 47 65 pathogenicity. Due to their regressive evolution by gene loss , mycoplasmas appear to lack 66 many of the common bacterial effectors and toxins used to interact with their hosts or to escape 67 the hosts' immune systems (6, 7). Lipoproteins have been proposed to be involved in both 68 aspects by using their cytoadherent properties and allowing antigenic variability through phase 69 or sequence variation (8). Other candidate virulence traits, such as the Mycoplasma Ig binding 70 protein-Mycoplasma Ig protease (MIB-MIP) system (9) and the hydrogen peroxide production 71 system (10) have been suggested, but not confirmed in vivo. 72

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High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237

Cited by 18 publications

References 42 publications

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

Removal of a subset of non-essential genes fully attenuates a highly virulentMycoplasmastrain

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