Whole-Genome Sequencing of Brevundimonas diminuta XGC1, Isolated from a Tuberculosis Patient in Gujarat, India

Ghosh, Arpita; Chandratre, Khyati; Chaudhary, Anjali; Chaudhary, Spandan; Badani, Namrata; Chaudhary, Pooja; Dhawan, Dipali; Vudathala, Srinivas; Chikara, Surendra K.

doi:10.1128/genomea.00686-15

Cited by 4 publications

(5 citation statements)

References 2 publications

(2 reference statements)

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“…IR18 was isolated in 2010 and IR165 isolated in 2016 from 1.5-month-old infant and five-years-old children, respectively. Our results revealed 50 contigs for each assembled fasta file with the average G+C% content of 67% and genome size 3161756 for IR18 and 3341181 for IR165 which is similar to the average genome size 3.3 Mbp reported for this strain [6].…”

Section: Resultssupporting

confidence: 83%

“…The GyrA QRDR of all available B. diminuta isolates were compared with P. aeruginosa and B. diminuta ATCC 11568, isolated in 1952 [44]. All B. diminuta isolates have Ala -83(92 in our genomes) and Met -87(96 in our genomes) mutation in the GyrA QRDR region, confirming the intrinsic quinolone resistance as previously reported for clinical isolates [5, 6].…”

Section: Discussionsupporting

confidence: 78%

“…They are usually used as test organisms for several purposes from validating reverse-osmosis filtration devices to mitigating the toxic effects of heavy metals on plant growth in contaminated soils [3, 4]. Brevundimonas vesicularis and Brevundimonas diminuta have also been isolated from clinical environments as opportunistic pathogens from patients with cancer cystic fibrosis and, tuberculosis [5, 6, 7]. Reports also showed some sporadic incidences caused by B. diminuta as an emerging opportunistic pathogen causing infection in the bloodstream, intravascular catheter, urinary tract, and pleural space [4].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Genomics of multidrug-resistant Brevundimonas diminuta collected from patients with pertussis-like symptoms

Safarchi

Nikbin

Lotfi

et al. 2021

Preprint

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Brevundinomas diminuta is known as an opportunistic pathogen and is rarely associated with invasive infections in humans causing infection in the different parts of the body. In this study, we identified three B. diminuta isolates from three patients with an initial diagnosis of pertussis. Isolates were confirmed using biochemical tests and 16s rRNA sequencing. All isolates were resistant to three different classes of antimicrobial drugs including ceftazidime and ciprofloxacin. We performed Illumina whole-genome sequencing for two isolates. The results showed an average genome size of 3.25 Mbp with the G+C content 67% with multiple predicted virulence factors and genes leading to antibiotic resistance. We found an open pan-genome with 1502 core genes by analysing 13 available global B. diminuta isolated from different environments such as water, soil, or gentamicin fermentation residue. In the phylogenetic analysis, our isolates were grouped with B. diminuta isolate collected from an oral cavity of a patient in the USA.

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Section: Resultssupporting

confidence: 83%

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

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Isolation and Genomics of multidrug-resistant Brevundimonas diminuta collected from patients with pertussis-like symptoms

Safarchi

Nikbin

Lotfi

et al. 2021

Preprint

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“…Brevundimonas spp. have been isolated from diverse aquatic environments, such as deep-sea sediment (3) and soil (4), and have also been isolated in clinical environments from eye, lung sputum, and urine collections (1, 5). Brevundimonas spp.…”

Section: Announcementmentioning

confidence: 99%

“…Brevundimonas spp. are noted to be potentially emerging pathogens causing nosocomial infections (1, 5).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Brevundimonas sp. Strain SGAir0440, Isolated from Indoor Air in Singapore

Purbojati

Drautz–Moses

Uchida

et al. 2019

Microbiol Resour Announc

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Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage

Liu

Chen

et al. 2017

Sci Rep

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Gentamicin, a broad spectrum antibiotic of the aminoglycoside class, is widely used for disease prevention of human beings as well as animals. Nowadays the environmental issue caused by the disposal of wastes containing gentamicin attracts increasing attention. In this study, a gentamicin degrading bacterial consortia named AMQD4, including Providencia vermicola, Brevundimonas diminuta, Alcaligenes sp. and Acinetobacter, was isolated from biosolids produced during gentamicin production for the removal of gentamicin in the environment. The component and structure of gentamicin have a great influence on its degradation and gentamicin C1a and gentamicin C2a were more prone to being degraded. AMQD4 could maintain relatively high gentamicin removal efficiency under a wide range of pH, especially in an alkaline condition. In addition, AMQD4 could remove 56.8% and 47.7% of gentamicin in unsterilized and sterilized sewage in a lab-scale experiment, respectively. And among the isolates in AMQD4, Brevundimonas diminuta BZC3 performed the highest gentamicin degradation about 50%. It was speculated that aac3iia was the gentamicin degradation gene and the main degradation product was 3′-acetylgentamicin. Our results suggest that AMQD4 and Brevundimonas diminuta BZC3 could be important candidates to the list of superior microbes for bioremediation of antibiotic pollution.

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Whole-Genome Sequencing of Brevundimonas diminuta XGC1, Isolated from a Tuberculosis Patient in Gujarat, India

Abstract: We report the draft genome of Brevundimonas diminuta strain XGC1, isolated from a tuberculosis-infected patient in Gujarat, India. This study also reveals that the B. diminuta XGC1 strain has acquired mutation to confer resistance to quinolone drugs.

Cited by 4 publications

References 2 publications

Isolation and Genomics of multidrug-resistant Brevundimonas diminuta collected from patients with pertussis-like symptoms

Isolation and Genomics of multidrug-resistant Brevundimonas diminuta collected from patients with pertussis-like symptoms

Complete Genome Sequence of Brevundimonas sp. Strain SGAir0440, Isolated from Indoor Air in Singapore

Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage

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