Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

Dadar, Maryam; Brangsch, Hanka; Alamian, Saeed; Neubauer, Heinrich; Wareth, Gamal

doi:10.1016/j.onehlt.2023.100483

Cited by 3 publications

(1 citation statement)

References 58 publications

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“…Brucellosis is one of the most important zoonotic diseases prevailing in the large and small ruminants of the Middle East, including Iran [ 10 , 16 , 26 ]. Vaccination is vital for preventing and eradicating infections and is the most economical control component [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Abnaroodheleh¹,

Emadi²,

Dashtipour³

et al. 2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Abnaroodheleh¹,

Emadi²,

Dashtipour³

et al. 2023

Heliyon

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Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5

Brangsch,

Horstkotte,

Melzer

2023

Sci Rep

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Human brucellosis cases are rare in non-endemic countries, such as Germany, where infections are predominantly caused by Brucella melitensis. The German National Reference Laboratory for Bovine, Porcine, Ovine and Caprine Brucellosis received a suspected Brucella sp. isolate from a patient for identification. Bacteriological tests and PCR-based diagnostics showed the isolate to be B. suis, but did not yield cohesive results regarding the biovar. Whole genome sequencing and subsequent genotyping was employed for a detailed characterization of the isolate and elucidating the reason for failure of the diagnostic PCR to correctly identify the biovar. The isolate was found to be B. suis bv. 5, a rare biovar with limited geographical distribution primarily found in the Northern Caucasus. Due to a deletion in one of the target regions of the diagnostic PCR, the isolate could not be correctly typed. Based on in silico genotyping it could be excluded that the isolate was identical to one of the B. suis bv. 5 reference strains. Here, we report a rare case of a B. suis bv. 5 field isolate. Furthermore, by reporting this finding, we want to make practitioners aware of possible misinterpretation of PCR results, as it cannot be excluded that the detected deletion is common among the B. suis bv. 5 community, as there is currently a lack of field isolates.

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Molecular epidemiological characteristics of osteoarthritis-associated Brucella melitensis in China: evidence from whole-genome sequencing-based analysis

Zhu,

Zhang,

Liang

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Brucellosis, developing complications including arthritis, spondylitis, sacroiliitis, and osteomyelitis, is one of the most common zoonotic diseases in the current world which causes economic losses to the livestock industry and is a great public health concern. Brucella melitensis are the main pathogen of brucellosis epidemics in China, most of which are located in northern China. However, there is limited knowledge about the epidemiology of osteoarthritis-associated brucellosis. This study was aimed to reveal the prevalence of osteoarthritis-associated brucellosis in Inner Mongolia and also to investigate the molecular characteristics of B. melitensis isolates. Methods and results In 2018, the osteoarthritis symptoms of brucellosis in the Brucellosis department of a hospital in Inner Mongolia were investigated. Twenty osteoarthritis-associated B. melitensis strains, isolated from the inpatients in Inner Mongolia during 2013–2017, were subjected to whole genome sequencing. The multilocus sequence type (MLST) and core genome SNP (cgSNP) analysis were conducted to detect molecular epidemiological characteristics. The incidence of brucellosis osteoarthritis symptoms in males (85/120, 70.8%) was significantly higher than that in females (35/120, 29.2%), and the age of patients was concentrated between 41 and 60 years old. In silico analyses indicated ST8 was the prevalent sequence type and the transmission of osteoarthritis-associated B. melitensis among different geographical areas. All strains carry virulence genes, including cgs, lpsA, manCoAg, pgm, pmm, virB4, wbdA and wboA. Conclusion Our study showed the close epidemiologically connection of osteoarthritis-associated B. melitensis strains in northern China. And ST8 was the prevalent sequence type which need our attention.

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Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

Cited by 3 publications

References 58 publications

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5

Molecular epidemiological characteristics of osteoarthritis-associated Brucella melitensis in China: evidence from whole-genome sequencing-based analysis

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