Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9

Abstract

Citrobacter freundii CD-9 is a Gram-negative bacteria sourced from factory sludge that can use fenvalerate as its sole carbon source and has a broad degradation spectrum for pyrethroid pesticides. The whole genome of CD-9 sequenced using Illumina HiSeq PE150 was reported in this study. The CD-9 genome size was 5.33 Mb and the G + C content was 51.55%. A total of 5291 coding genes, 9 5s-rRNA, and 79 tRNA were predicted bioinformatically. 3586 genes annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that can be involved in 173 metabolic pathways, including various microbial metabolic pathways that degrade exogenous chemicals, especially those that degrade aromatic compounds, and also produce a variety of bioactive substances. Fifty genes related to pyrethroid degradation were identified in the C. freundii CD-9 genome, including 9 dioxygenase, 25 hydrolase, and 16 esterase genes. Notably, RT-qPCR results showed that from the predicted 13 genes related to fenvalerate degradation, the expression of six genes, including esterase, HAD family hydrolase, lipolytic enzyme, and gentisic acid dioxygenase, was induced in the presence of fenvalerate. In this study, the key genes and degradation mechanism of C. freundii CD-9 were analyzed and the results provide scientific evidence to support its application in environmental bioremediation. It can establish application models for different environmental pollution management by constructing genetically engineered bacteria for efficient fenvalerate or developing enzyme formulations that can be industrially produced.

Distinct gut bacterial composition in Anoplophora glabripennis reared on two host plants

“…This indicates that Gibbsiella might participate in fermenting glucose into lipids that further promote the growth and development of A. glabripennis larvae. Citrobacter and Klebsiella can degrade aromatic compounds (Ammar et al, 2005;Kraigher and Mandic-Mulec, 2020;Zhou et al, 2022). Some functions of gut bacteria are related to the nitrogen cycle, which is essential for insect growth and development.…”

Application of microbial agents in organic solid waste composting: a review

“…The discovery of these genes has provided the basis for the development of new biodegrades, biopesticides and so on. 99 Researchers have used mesophilic bacteria for fermentation to produce many bioproducts, such as biofertilizers, biopesticides and bioactive substances (e.g., active polysaccharides, amino acids, peptides and proteins). Room temperature fermentation technologies are also involved in the food industry.…”

“…The expression and activity of functional genes has an important impact on the growth and metabolism of the microorganism. The discovery of these genes has provided the basis for the development of new biodegrades, biopesticides and so on 99 …”